plenti cmvtight egfp blast (Addgene inc)
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Plenti Cmvtight Egfp Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basic+local+alignment+search+tool+software+program/pLenti+CMVtight+eGFP+Blast+(w782-1)+(Plasmid+%2326584)/pm38692097-189-9-13
Average 93 stars, based on 2 article reviews
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Plasmid Preparation:Article Title: The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint Article Snippet: .. pLenti CMVtight eGFP Blast (w782-1); pNC1745 , Eric Campeau , Article Title: The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methionine-free DMEM Sigma Cat#D0422 Sodium Pyruvate ThermoFisher Cat#11360070 L-cysteine Sigma Cat#C7352 L-methionine Sigma Cat#M9625 Doxycycline Fisher Cat#BP2653 Auxin Sigma Cat#I5148 AGI-24512 (MAT2Ai) MCE Cat#HY-112130 PBS + CaCl2 + MgCl2 Sigma Cat#D8662 Gibson Assembly Master Mix NEB Cat#M5510A Trypsin Sigma Cat#T4174 Cycloheximide Sigma Cat#01810-1G cRNA Sigma Cat#R6625 4SU ThermoFisher Cat#J60679.MD Biotin-HPDP ThermoFisher Cat#21341 poly(A) RNA Sigma Cat#P9403 ssDNA Sigma Cat#S3126 Polybrene Sigma Cat#H9268 Antarctic phosphatase NEB Cat#M0289S Critical commercial assays CellTiter-Glo Reagent ProMega Cat#G7570 NEBNext Magnesium RNA Fragmentation Module NEB Cat#E6150S RiboMinus Eukaryote System V2 ThermoFisher A15026 NEBNext Small RNA Library Prep Set for Illumina NEB E7330S NEBNext Multiplex Oligos for Illumina, Index primers set 1 NEB E7335S NEBNext Multiplex Oligos for Illumina, Index primers set 2 NEB E7500S Monarch Spin PCR & DNA Cleanup Kit NEB T1130S Deposited data Raw and analyzed GLORI-Seq data This paper GEO: GSE296342 Experimental models: Cell lines HEK 293T Dr. Joshua Mendell RRID: CVCL_0063 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 Oligonucleotides See Table S5 This paper, ordered from Millipore N/A Recombinant DNA pMD2.G Didier Trono Addgene plasmid # 12259 psPAX2 Didier Trono Addgene plasmid # 12260 lentiCRISPRv2 Sanjana et al. 76 Addgene plasmid # 52961 pX459 Ran et al. 77 Addgene plasmid # 62988 pMGS46 Sathyan et al. 78 Addgene plasmid # 126580 pBluescript SK+; pNC65 Stratagene, La Jolla, CA Cat#212205 pcDNA-flag; pNC226 Sahin et al. 79 N/A pOG44; pNC450 ThermoFisher Cat#V600520 Talen L; pNC1050 Sajana et al. 80 Addgene plasmid # 35431 Talen R; pNC1051 Sajana et al. 80 Addgene plasmid # 35432 WT flag-METTL16; pNC1099 Pendleton et al. 21 N/A (Continued on next page) Cell Reports 44, 115966, July 22, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER catalytic dead N184A flag-METTL16; pNC1145 Doxtader et al. 29 N/A pLenti-sgMTAP #1; pNC1151 This paper N/A pLenti-sgMTAP #2; pNC1152 This paper N/A pLenti-sgMTAP #3; pNC1153 This paper N/A pLenti-CRISPR-vs-NT; pNC1154 Dr. John Schoggins N/A hyperactive K163A flag-mettl16; pNC1298 Doxtader et al. 29 N/A pBS-Flag-mAID-T2A-Hyg; pNC1393 This paper N/A M16-C-sg1/2-Hyg; pNC1408 This paper N/A Cas9 and guide; pNC1410 This paper N/A M16-C-sg2-Flag-AID-Donor; pNC1486 This paper N/A pBS-AAVS1-TetOn-Hyg-FRT; pNC1537 Genscript, see Table S6 for sequence N/A pBS-AAVS1-TetON-ARF16 PB1 - TIR1; pNC1549 This paper N/A WT myc-METTL16; pNC1680 This paper N/A EV, aka myc-STOP; pNC1688 This paper N/A catalytic dead N184A myc-METTL16; pNC1692 This paper N/A hyperactive K163A myc-METTL16; pNC1693 This paper N/A Flag-mAID-T2A-Hyg Insert Genewiz, see Table S6 for sequence N/A pBS-hAAVS1-TetOFF-HA-OsTIR1-HYGFRT48; pNC1746 This paper N/A pLenti CMVtight eGFP Blast (w782-1); Polymerase Chain Reaction:Article Title: Grainyhead-like-2, an epithelial master programmer, promotes interferon induction and suppresses breast cancer recurrence. Article Snippet: IRF1 and IRF3–5D sequences were PCR amplified from clones kindly provided by Alan Brasier (University of Wisconsin) (Yang et al., 2017) or John Hiscott (Pasteur Institute, Rome, via Matthew Frieman, University of Maryland) (Romieu-Mourez et al., 2006). .. PCR fragments were subcloned into the BstBI-XbaI site of Sequencing:Article Title: Grainyhead-like-2, an epithelial master programmer, promotes interferon induction and suppresses breast cancer recurrence. Article Snippet: IRF1 and IRF3–5D sequences were PCR amplified from clones kindly provided by Alan Brasier (University of Wisconsin) (Yang et al., 2017) or John Hiscott (Pasteur Institute, Rome, via Matthew Frieman, University of Maryland) (Romieu-Mourez et al., 2006). .. PCR fragments were subcloned into the BstBI-XbaI site of Article Title: The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methionine-free DMEM Sigma Cat#D0422 Sodium Pyruvate ThermoFisher Cat#11360070 L-cysteine Sigma Cat#C7352 L-methionine Sigma Cat#M9625 Doxycycline Fisher Cat#BP2653 Auxin Sigma Cat#I5148 AGI-24512 (MAT2Ai) MCE Cat#HY-112130 PBS + CaCl2 + MgCl2 Sigma Cat#D8662 Gibson Assembly Master Mix NEB Cat#M5510A Trypsin Sigma Cat#T4174 Cycloheximide Sigma Cat#01810-1G cRNA Sigma Cat#R6625 4SU ThermoFisher Cat#J60679.MD Biotin-HPDP ThermoFisher Cat#21341 poly(A) RNA Sigma Cat#P9403 ssDNA Sigma Cat#S3126 Polybrene Sigma Cat#H9268 Antarctic phosphatase NEB Cat#M0289S Critical commercial assays CellTiter-Glo Reagent ProMega Cat#G7570 NEBNext Magnesium RNA Fragmentation Module NEB Cat#E6150S RiboMinus Eukaryote System V2 ThermoFisher A15026 NEBNext Small RNA Library Prep Set for Illumina NEB E7330S NEBNext Multiplex Oligos for Illumina, Index primers set 1 NEB E7335S NEBNext Multiplex Oligos for Illumina, Index primers set 2 NEB E7500S Monarch Spin PCR & DNA Cleanup Kit NEB T1130S Deposited data Raw and analyzed GLORI-Seq data This paper GEO: GSE296342 Experimental models: Cell lines HEK 293T Dr. Joshua Mendell RRID: CVCL_0063 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 Oligonucleotides See Table S5 This paper, ordered from Millipore N/A Recombinant DNA pMD2.G Didier Trono Addgene plasmid # 12259 psPAX2 Didier Trono Addgene plasmid # 12260 lentiCRISPRv2 Sanjana et al. 76 Addgene plasmid # 52961 pX459 Ran et al. 77 Addgene plasmid # 62988 pMGS46 Sathyan et al. 78 Addgene plasmid # 126580 pBluescript SK+; pNC65 Stratagene, La Jolla, CA Cat#212205 pcDNA-flag; pNC226 Sahin et al. 79 N/A pOG44; pNC450 ThermoFisher Cat#V600520 Talen L; pNC1050 Sajana et al. 80 Addgene plasmid # 35431 Talen R; pNC1051 Sajana et al. 80 Addgene plasmid # 35432 WT flag-METTL16; pNC1099 Pendleton et al. 21 N/A (Continued on next page) Cell Reports 44, 115966, July 22, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER catalytic dead N184A flag-METTL16; pNC1145 Doxtader et al. 29 N/A pLenti-sgMTAP #1; pNC1151 This paper N/A pLenti-sgMTAP #2; pNC1152 This paper N/A pLenti-sgMTAP #3; pNC1153 This paper N/A pLenti-CRISPR-vs-NT; pNC1154 Dr. John Schoggins N/A hyperactive K163A flag-mettl16; pNC1298 Doxtader et al. 29 N/A pBS-Flag-mAID-T2A-Hyg; pNC1393 This paper N/A M16-C-sg1/2-Hyg; pNC1408 This paper N/A Cas9 and guide; pNC1410 This paper N/A M16-C-sg2-Flag-AID-Donor; pNC1486 This paper N/A pBS-AAVS1-TetOn-Hyg-FRT; pNC1537 Genscript, see Table S6 for sequence N/A pBS-AAVS1-TetON-ARF16 PB1 - TIR1; pNC1549 This paper N/A WT myc-METTL16; pNC1680 This paper N/A EV, aka myc-STOP; pNC1688 This paper N/A catalytic dead N184A myc-METTL16; pNC1692 This paper N/A hyperactive K163A myc-METTL16; pNC1693 This paper N/A Flag-mAID-T2A-Hyg Insert Genewiz, see Table S6 for sequence N/A pBS-hAAVS1-TetOFF-HA-OsTIR1-HYGFRT48; pNC1746 This paper N/A pLenti CMVtight eGFP Blast (w782-1); Software:Article Title: The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methionine-free DMEM Sigma Cat#D0422 Sodium Pyruvate ThermoFisher Cat#11360070 L-cysteine Sigma Cat#C7352 L-methionine Sigma Cat#M9625 Doxycycline Fisher Cat#BP2653 Auxin Sigma Cat#I5148 AGI-24512 (MAT2Ai) MCE Cat#HY-112130 PBS + CaCl2 + MgCl2 Sigma Cat#D8662 Gibson Assembly Master Mix NEB Cat#M5510A Trypsin Sigma Cat#T4174 Cycloheximide Sigma Cat#01810-1G cRNA Sigma Cat#R6625 4SU ThermoFisher Cat#J60679.MD Biotin-HPDP ThermoFisher Cat#21341 poly(A) RNA Sigma Cat#P9403 ssDNA Sigma Cat#S3126 Polybrene Sigma Cat#H9268 Antarctic phosphatase NEB Cat#M0289S Critical commercial assays CellTiter-Glo Reagent ProMega Cat#G7570 NEBNext Magnesium RNA Fragmentation Module NEB Cat#E6150S RiboMinus Eukaryote System V2 ThermoFisher A15026 NEBNext Small RNA Library Prep Set for Illumina NEB E7330S NEBNext Multiplex Oligos for Illumina, Index primers set 1 NEB E7335S NEBNext Multiplex Oligos for Illumina, Index primers set 2 NEB E7500S Monarch Spin PCR & DNA Cleanup Kit NEB T1130S Deposited data Raw and analyzed GLORI-Seq data This paper GEO: GSE296342 Experimental models: Cell lines HEK 293T Dr. Joshua Mendell RRID: CVCL_0063 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 Oligonucleotides See Table S5 This paper, ordered from Millipore N/A Recombinant DNA pMD2.G Didier Trono Addgene plasmid # 12259 psPAX2 Didier Trono Addgene plasmid # 12260 lentiCRISPRv2 Sanjana et al. 76 Addgene plasmid # 52961 pX459 Ran et al. 77 Addgene plasmid # 62988 pMGS46 Sathyan et al. 78 Addgene plasmid # 126580 pBluescript SK+; pNC65 Stratagene, La Jolla, CA Cat#212205 pcDNA-flag; pNC226 Sahin et al. 79 N/A pOG44; pNC450 ThermoFisher Cat#V600520 Talen L; pNC1050 Sajana et al. 80 Addgene plasmid # 35431 Talen R; pNC1051 Sajana et al. 80 Addgene plasmid # 35432 WT flag-METTL16; pNC1099 Pendleton et al. 21 N/A (Continued on next page) Cell Reports 44, 115966, July 22, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER catalytic dead N184A flag-METTL16; pNC1145 Doxtader et al. 29 N/A pLenti-sgMTAP #1; pNC1151 This paper N/A pLenti-sgMTAP #2; pNC1152 This paper N/A pLenti-sgMTAP #3; pNC1153 This paper N/A pLenti-CRISPR-vs-NT; pNC1154 Dr. John Schoggins N/A hyperactive K163A flag-mettl16; pNC1298 Doxtader et al. 29 N/A pBS-Flag-mAID-T2A-Hyg; pNC1393 This paper N/A M16-C-sg1/2-Hyg; pNC1408 This paper N/A Cas9 and guide; pNC1410 This paper N/A M16-C-sg2-Flag-AID-Donor; pNC1486 This paper N/A pBS-AAVS1-TetOn-Hyg-FRT; pNC1537 Genscript, see Table S6 for sequence N/A pBS-AAVS1-TetON-ARF16 PB1 - TIR1; pNC1549 This paper N/A WT myc-METTL16; pNC1680 This paper N/A EV, aka myc-STOP; pNC1688 This paper N/A catalytic dead N184A myc-METTL16; pNC1692 This paper N/A hyperactive K163A myc-METTL16; pNC1693 This paper N/A Flag-mAID-T2A-Hyg Insert Genewiz, see Table S6 for sequence N/A pBS-hAAVS1-TetOFF-HA-OsTIR1-HYGFRT48; pNC1746 This paper N/A pLenti CMVtight eGFP Blast (w782-1); |
