pcc 09 (Addgene inc)
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Addgene inc
pcc 09
Pcc 09, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding/pCC_09+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE+(Plasmid+%23139094)/pm38472198-346-2-3
Average 93 stars, based on 9 article reviews
Pcc 09, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding/pCC_09+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE+(Plasmid+%23139094)/pm38472198-346-2-3
Average 93 stars, based on 9 article reviews
pcc 09 - by Bioz Stars,
2026-09
93/100 stars
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Amplification:Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers. Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Clone Assay:Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers. Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Modification:Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Article Title: Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer Article Snippet: .. pCC_01 and Article Title: Identifying regulators of aberrant stem cell and differentiation activity in colorectal cancer using a dual endogenous reporter system Article Snippet: .. pCC_01 and Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers. Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Ligation:Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers. Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the other:Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers. Article Snippet: The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria. |