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1x barcode perm buffer  (fluidigm)


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    Structured Review

    fluidigm 1x barcode perm buffer
    1x Barcode Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoding+perm+buffer/Maxpar+Barcode+Perm+Buffer/pm40701154-827-8-12
    Average 95 stars, based on 75 article reviews
    1x barcode perm buffer - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Saline:

    Article Title: Early immune responses anticipate HIV rebound and precede viral control
    Article Snippet: .. Dead cells were marked by incubating the samples for one minute with 25 mM cisplatin (Sigma-Aldrich) in phosphate buffered saline (PBS) with EDTA, performed surface staining with metal-tagged antibodies in PBS with 0.5% bovine serum albumin (BSA) for 30 min at room temperature, fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Standard BioTools, South San Francisco, CA, USA), combined up to twenty barcoded samples into a single tube, performed intracellular staining with antibodies diluted in eBioscience Foxp3/Transcription Factor kit perm wash (Thermo Fisher Scientific), fixed cells in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in the presence of a DNA intercalator, and then washed and ran cells on the Standard BioTools CyTOF 2 mass Cytometer within one week of staining. ..

    Staining:

    Article Title: Early immune responses anticipate HIV rebound and precede viral control
    Article Snippet: .. Dead cells were marked by incubating the samples for one minute with 25 mM cisplatin (Sigma-Aldrich) in phosphate buffered saline (PBS) with EDTA, performed surface staining with metal-tagged antibodies in PBS with 0.5% bovine serum albumin (BSA) for 30 min at room temperature, fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Standard BioTools, South San Francisco, CA, USA), combined up to twenty barcoded samples into a single tube, performed intracellular staining with antibodies diluted in eBioscience Foxp3/Transcription Factor kit perm wash (Thermo Fisher Scientific), fixed cells in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in the presence of a DNA intercalator, and then washed and ran cells on the Standard BioTools CyTOF 2 mass Cytometer within one week of staining. ..

    Article Title: Granulin+ Macrophages Promote Lineage Plasticity in Prostate Cancer Through Paracrine Signaling Loops.
    Article Snippet: Live/dead staining was performed using 2 μM cisplatin (Catalog No. 201064, 783 Fluidigm, South San Francisco, CA). .. Cells were quenched with Cell Staining Buffer 784 (CSB; Catalog No. 201057, Fluidigm). .. Subsequently, cells were first stained and fixed 785 with Fix-I buffer (Catalog No. 201061, Fluidigm); then, they were secondarily stained 786 using the Cell-IDTM 20-Plex Pd Barcoding Kit (Catalog No. 201060, Fluidigm) to 787 reduce sample cross-reactivity.

    Electron Microscopy:

    Article Title: Early immune responses anticipate HIV rebound and precede viral control
    Article Snippet: .. Dead cells were marked by incubating the samples for one minute with 25 mM cisplatin (Sigma-Aldrich) in phosphate buffered saline (PBS) with EDTA, performed surface staining with metal-tagged antibodies in PBS with 0.5% bovine serum albumin (BSA) for 30 min at room temperature, fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Standard BioTools, South San Francisco, CA, USA), combined up to twenty barcoded samples into a single tube, performed intracellular staining with antibodies diluted in eBioscience Foxp3/Transcription Factor kit perm wash (Thermo Fisher Scientific), fixed cells in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in the presence of a DNA intercalator, and then washed and ran cells on the Standard BioTools CyTOF 2 mass Cytometer within one week of staining. ..

    Cytometry:

    Article Title: Early immune responses anticipate HIV rebound and precede viral control
    Article Snippet: .. Dead cells were marked by incubating the samples for one minute with 25 mM cisplatin (Sigma-Aldrich) in phosphate buffered saline (PBS) with EDTA, performed surface staining with metal-tagged antibodies in PBS with 0.5% bovine serum albumin (BSA) for 30 min at room temperature, fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Standard BioTools, South San Francisco, CA, USA), combined up to twenty barcoded samples into a single tube, performed intracellular staining with antibodies diluted in eBioscience Foxp3/Transcription Factor kit perm wash (Thermo Fisher Scientific), fixed cells in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in the presence of a DNA intercalator, and then washed and ran cells on the Standard BioTools CyTOF 2 mass Cytometer within one week of staining. ..



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    Image Search Results


    Journal: Bio-protocol

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood

    doi: 10.21769/BioProtoc.5512

    Figure Lengend Snippet:

    Article Snippet: Maxpar 10× barcode perm concentrate (50 mL) (Standard BioTools, catalog number: 201057) 17.

    Techniques: Concentration Assay