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Oxford Nanopore 1d native barcoding genomic dna kit
1d Native Barcoding Genomic Dna Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+kit/Native+Barcoding+Expansion+1-12/pmc10569574-99-11-21
Average 96 stars, based on 1 article reviews
1d native barcoding genomic dna kit - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Sequencing:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Article Title: Rapid whole genome sequencing methods for RNA viruses.
Article Snippet: .. The following procedure, which involved branching, end-prepping and ligating sequencing adapters to the amplified cDNA, was modified from the protocols of the premium whole genome amplification Frontiers in Microbiology 05 frontiersin.org protocol (version: WAL_9070_v109_revQ_14Aug2019) and Native Barcoding genomic DNA (NBE_9065_v109_revAK_14Aug2019) using a ligation sequence kit (SQK-LSK109), Native Barcoding Expansion 1-12 and 12-24 (EXP-NBD104 and EXP-NBD114) supplied by the Oxford Nanopore Technologies. .. The amplified cDNA was digested using T7 endonuclease I (catalog number M0302S, New England BioLabs), to remove the branching.

Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation
Article Snippet: Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346-086; Invitrogen). .. MinION sequencing was carried out following the manufacturer’s instructions using the 1D Native barcoding genomic DNA Kit (Nanopore, EXP-NBD104 and SQK-LSK109, Oxford Nanopore-technology (ONT)) on a Spot-ON flow cell (R9.4.1; ONT). ..

Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation
Article Snippet: Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [ ]. .. MinION sequencing was carried out following the manufacturer’s instructions using the 1D Native barcoding genomic DNA Kit (Nanopore, EXP-NBD104 and SQK-LSK109, Oxford Nanopore-technology (ONT)) on a Spot-ON flow cell (R9.4.1; ONT). ..

Amplification:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Article Title: Rapid whole genome sequencing methods for RNA viruses.
Article Snippet: .. The following procedure, which involved branching, end-prepping and ligating sequencing adapters to the amplified cDNA, was modified from the protocols of the premium whole genome amplification Frontiers in Microbiology 05 frontiersin.org protocol (version: WAL_9070_v109_revQ_14Aug2019) and Native Barcoding genomic DNA (NBE_9065_v109_revAK_14Aug2019) using a ligation sequence kit (SQK-LSK109), Native Barcoding Expansion 1-12 and 12-24 (EXP-NBD104 and EXP-NBD114) supplied by the Oxford Nanopore Technologies. .. The amplified cDNA was digested using T7 endonuclease I (catalog number M0302S, New England BioLabs), to remove the branching.

Construct:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Modification:

Article Title: Rapid whole genome sequencing methods for RNA viruses.
Article Snippet: .. The following procedure, which involved branching, end-prepping and ligating sequencing adapters to the amplified cDNA, was modified from the protocols of the premium whole genome amplification Frontiers in Microbiology 05 frontiersin.org protocol (version: WAL_9070_v109_revQ_14Aug2019) and Native Barcoding genomic DNA (NBE_9065_v109_revAK_14Aug2019) using a ligation sequence kit (SQK-LSK109), Native Barcoding Expansion 1-12 and 12-24 (EXP-NBD104 and EXP-NBD114) supplied by the Oxford Nanopore Technologies. .. The amplified cDNA was digested using T7 endonuclease I (catalog number M0302S, New England BioLabs), to remove the branching.

Whole Genome Amplification:

Article Title: Rapid whole genome sequencing methods for RNA viruses.
Article Snippet: .. The following procedure, which involved branching, end-prepping and ligating sequencing adapters to the amplified cDNA, was modified from the protocols of the premium whole genome amplification Frontiers in Microbiology 05 frontiersin.org protocol (version: WAL_9070_v109_revQ_14Aug2019) and Native Barcoding genomic DNA (NBE_9065_v109_revAK_14Aug2019) using a ligation sequence kit (SQK-LSK109), Native Barcoding Expansion 1-12 and 12-24 (EXP-NBD104 and EXP-NBD114) supplied by the Oxford Nanopore Technologies. .. The amplified cDNA was digested using T7 endonuclease I (catalog number M0302S, New England BioLabs), to remove the branching.

Article Title: Aneuploidy detection in pooled polar bodies using rapid nanopore sequencing
Article Snippet: The size-selected DNA was eluted in 25 μl Buffer EB (Qiagen, #19,086). .. For the “native barcoding genomic DNA” workflow (Oxford Nanopore, SQK-LSK109), 80 ng of size-selected WGA-DNA was processed with DNA repair and end-prep with the “NEBNext Companion Module” (NEB, #E7180S) and purified with onefold AMPure XP Beads (Beckman Coulter, #A63881). .. Afterwards, native barcode (Oxford Nanopore, #EXP-NBD104, #EXP-NBD114) and NEB Blunt/TA Ligase Master Mix (NEB, #M0367L) were added, and the purification step was repeated.

Ligation:

Article Title: Rapid whole genome sequencing methods for RNA viruses.
Article Snippet: .. The following procedure, which involved branching, end-prepping and ligating sequencing adapters to the amplified cDNA, was modified from the protocols of the premium whole genome amplification Frontiers in Microbiology 05 frontiersin.org protocol (version: WAL_9070_v109_revQ_14Aug2019) and Native Barcoding genomic DNA (NBE_9065_v109_revAK_14Aug2019) using a ligation sequence kit (SQK-LSK109), Native Barcoding Expansion 1-12 and 12-24 (EXP-NBD104 and EXP-NBD114) supplied by the Oxford Nanopore Technologies. .. The amplified cDNA was digested using T7 endonuclease I (catalog number M0302S, New England BioLabs), to remove the branching.

Article Title: Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long- and Short-read Multi-metagenomic Sequencing Studies.
Article Snippet: DNA was quantified via Nanodrop 2000/2000c (ThermoFisher Scientific, Wilmington, DE) and the volume was determinated Please cite this article as: J. Rehner, G. P. Schmartz, L. Groeger et al., Systematic Cr Multi-metagenomic Sequencing Studies, Genomics Proteomics Bioinformatics, https by using a pipette (Table S2). .. The library preparation was conducted according to the protocol ‘‘Native barcoding genomic DNA (with EXP-NBD 104)” provided by Oxford Nanopore Technologies (ONT), with the exception of the barcode ligation step and further the adapter ligation step for which the ligation mix was incubated for 15 min at room temperature instead of 10 min. ..

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes.
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.

Adapter Ligation:

Article Title: Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long- and Short-read Multi-metagenomic Sequencing Studies.
Article Snippet: DNA was quantified via Nanodrop 2000/2000c (ThermoFisher Scientific, Wilmington, DE) and the volume was determinated Please cite this article as: J. Rehner, G. P. Schmartz, L. Groeger et al., Systematic Cr Multi-metagenomic Sequencing Studies, Genomics Proteomics Bioinformatics, https by using a pipette (Table S2). .. The library preparation was conducted according to the protocol ‘‘Native barcoding genomic DNA (with EXP-NBD 104)” provided by Oxford Nanopore Technologies (ONT), with the exception of the barcode ligation step and further the adapter ligation step for which the ligation mix was incubated for 15 min at room temperature instead of 10 min. ..

Incubation:

Article Title: Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long- and Short-read Multi-metagenomic Sequencing Studies.
Article Snippet: DNA was quantified via Nanodrop 2000/2000c (ThermoFisher Scientific, Wilmington, DE) and the volume was determinated Please cite this article as: J. Rehner, G. P. Schmartz, L. Groeger et al., Systematic Cr Multi-metagenomic Sequencing Studies, Genomics Proteomics Bioinformatics, https by using a pipette (Table S2). .. The library preparation was conducted according to the protocol ‘‘Native barcoding genomic DNA (with EXP-NBD 104)” provided by Oxford Nanopore Technologies (ONT), with the exception of the barcode ligation step and further the adapter ligation step for which the ligation mix was incubated for 15 min at room temperature instead of 10 min. ..

other:

Article Title: Endogenous Viral Elements in Shrew Genomes Provide Insights into Pestivirus Ancient History
Article Snippet: The Oxford Nanopore Technologies (ONT) 1D Native barcoding genomic DNA protocol was used without the DNA fragmentation step and the barcoded amplicons were loaded onto the MinION device.

Purification:

Article Title: Aneuploidy detection in pooled polar bodies using rapid nanopore sequencing
Article Snippet: The size-selected DNA was eluted in 25 μl Buffer EB (Qiagen, #19,086). .. For the “native barcoding genomic DNA” workflow (Oxford Nanopore, SQK-LSK109), 80 ng of size-selected WGA-DNA was processed with DNA repair and end-prep with the “NEBNext Companion Module” (NEB, #E7180S) and purified with onefold AMPure XP Beads (Beckman Coulter, #A63881). .. Afterwards, native barcode (Oxford Nanopore, #EXP-NBD104, #EXP-NBD114) and NEB Blunt/TA Ligase Master Mix (NEB, #M0367L) were added, and the purification step was repeated.

Multiplexing:

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes.
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.



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