cell id 20 plex pd barcoding kit (fluidigm)

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fluidigm cell id 20 plex pd barcoding kit
Cell Id 20 Plex Pd Barcoding Kit, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+kit/Cell-ID+20-Plex+Pd+Barcoding+Kit/ppr0568348-59-11-16
Average 96 stars, based on 650 article reviews

cell id 20 plex pd barcoding kit - by Bioz Stars, 2026-10

96/100 stars

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Staining:

Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: The conjugates were then diluted 1:1 with a PBS-based Antibody Stabilizer (Boca Scientific) supplemented with 0.05% sodium azide, and stored at 4 ◦ C until use. .. CyTOF cell barcoding and staining Cell barcoding was performed using the Cell-ID 20-Plex Pd Barcoding Kit in accordance with manufacturer’s guidelines (Standard BioTools). .. Briefly, for each sample, a total of 1-3 million cells was washed twice with Barcode Perm Buffer (Standard BioTools).

Article Title: Homoharringtonine exhibits senotherapeutic activity that mitigates diet- and age-associated obesity and insulin resistance and extends lifespan in mice
Article Snippet: The flow-through was centrifuged for 5 min at 400 x g. After removing the floating adipocyte fraction and supernatant, red blood cell lysis buffer (Sigma-Aldrich, #R7757) was added and incubated at room temperature for 2-3 min. .. The red blood cell lysis buffer is neutralized with DMEM/F12 media (supplemented with 10% FBS) and centrifuged for 5 min at 400 x g. The dissociated cells from adipose tissue or spleen were washed in a cell staining medium (CSM) and each sample was barcoded before staining according to the protocol described in the Cell-ID 20-Plex Pd Barcoding kit (Fluidigm, San Francisco, CA). ..

Article Title: Mass Cytometry Workflow to Achieve High‐Dimensional Immunophenotyping in Resource‐Limited or Decentralized Environments
Article Snippet: .. Foxp3/Transcription Factor Staining Buffer Set (eBioscience, cat. no. 00‐5523‐00) Cell‐ID 20‐Plex Pd Barcoding Kit (Standard BioTools, cat. no. 201060) CyTOF antibodies for intracellular targets (see Table ) FACS buffer (see recipe) Maxpar Cell Acquisition Solution (Standard BioTools, cat no. 201240) 5‐ml round‐bottom tubes 15‐ or 50‐ml centrifuge tubes as needed (see step 10) Ultrafree Centrifugal Filter (Millipore, cat. no. UFC30DV25) 2‐ml screw‐cap micro tubes (cryovials), sterile (Sarstedt, cat. no. 72.694.005) CoolCell LX Freezing Container (Corning, cat. no. 432002) −80°C freezer 5‐ml Falcon Round‐Bottom Tubes with Cell Strainer Cap (STEMCELL, cat. no. 38030) EQ Six Element Calibration Beads (100 ml; Standard BioTools, cat. no. 201245) CyTOF instrument: e.g., Helios system with standard equipment and CyTOF software ..

Article Title: Granulin+ Macrophages Promote Lineage Plasticity in Prostate Cancer Through Paracrine Signaling Loops.
Article Snippet: Cells were quenched with Cell Staining Buffer 784 (CSB; Catalog No. 201057, Fluidigm). .. Subsequently, cells were first stained and fixed 785 with Fix-I buffer (Catalog No. 201061, Fluidigm); then, they were secondarily stained 786 using the Cell-IDTM 20-Plex Pd Barcoding Kit (Catalog No. 201060, Fluidigm) to 787 reduce sample cross-reactivity. .. Antibodies were labeled with Maxpar® x8 Polymer Kits 788 (Catalog No. 201302, Fluidigm) following the manufacturer’s protocol.

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Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
Article Snippet: Each barcode was resuspended in MaxPar Fix & Perm Buffer (201067, Standard Biotools) and transferred to the corresponding sample, followed by incubation for 30 min at room temperature and three washes with CSB at 800 x g for 5 min. Barcoded cells were then pooled together and incubated with Cell-ID Intercalator-Ir (201192 A, Standard Biotools) diluted in MaxPar Fix & Perm Buffer at a final concentration of 50 nM and stored at 4◦C overnight.

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