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bac microarray cytochip focus constitutional  (Illumina Inc)


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    Illumina Inc bac microarray cytochip focus constitutional
    Bac Microarray Cytochip Focus Constitutional, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bac+microarray/cytochip+focus+constitutional+microarrays/pmc08270131-80-10-19
    Average 90 stars, based on 1 article reviews
    bac microarray cytochip focus constitutional - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Mucinous spindle and tubular renal cell carcinoma: analysis of chromosomal aberration pattern of low-grade, high-grade, and overlapping morphologic variant with papillary renal cell carcinoma.
    Article Snippet: ☆ The study was supported by the Charles University R and by the project CZ.1.05/2.1.00/03.0076 from European ☆☆ Disclosure of conflict of interest: All authors declare ⁎ Corresponding author at: Department of Pathology, ulty and Charles University Hospital Plzen, Alej Svobody 8 Tel.. : +420377104643; fax: +420377104650.. E-mail address: hes@medima.cz (O. Hes). http://dx.doi.org/10.1016/j.anndiagpath.2015.04.004 1092-9134/© 2015 Elsevier Inc. All rights reserved.

    Article Title: Cytogenetic Investigation in 136 Consecutive Stillbirths: Does the Tissue Type Affect the Success Rate of Chromosomal Microarray Analysis and Karyotype?
    Article Snippet: Background: Chromosomal anomalies are a recognized cause of stillbirth, accounting for 6–17% of the cases.. As a diagnostic laboratory method in this setting, conventional karyotyping has two main drawbacks: the need for viable fetal cells in a dead fetus and its limited resolution as compared to alternative techniques.. Objective: To assess the effectiveness of cytogenetic analysis in stillbirths between different testing methods and different sampled tissues.

    Article Title: Routine use of array comparative genomic hybridization (aCGH) as standard approach for prenatal diagnosis of chromosomal abnormalities. Clinical experience of 1763 prenatal cases.
    Article Snippet: Objective This study aims to evaluate the diagnostic yield of comparative genomic hybridization microarrays (aCGH) and compare it with conventional karyotype analysis of standard >5-Mb resolution.. Method A total of 1763 prenatal samples were analyzed by aCGH (CytoChip Focus Constitutional microarrays, BlueGnome, Cambridge).. The diagnostic yield of chromosomal abnormalities detected by aCGH was assessed, compared with conventional karyotype analysis.

    Article Title: Have maternal or paternal ages any impact on the prenatal incidence of genomic copy number variants associated with fetal structural anomalies?
    Article Snippet: During the first four years (2012–2015) of the study, a BAC (bacterial artificial chromosome) microarray (CytoChip Focus Constitutional, BlueGenome, Illumina, San Diego, California, USA) was used with a 1 Mb resolution along the whole genome and 100 kb in 143 constitutional regions associated with pathology.

    BAC Assay:

    Article Title: Prenatal diagnosis of 1p34.3 interstitial microdeletion by aCGH in a fetus with jaw bone abnormalities
    Article Snippet: .. Molecular karyotype analysis by array CGH using “Illumina Cytochip Focus Constitutional array with BAC technology” was performed on DNA isolated from uncultured amniocytes according to the manufacturer’s protocol. .. The Illumina Cytochip Focus Constitutional array is a commercially available whole-genome BAC array with a median resolution of 0.5−1 Mb. (Cytochip Focus Constitutional, Illumina).

    Article Title: Genetic Counseling and Management: The First Study to Report NIPT Findings in a Romanian Population
    Article Snippet: .. Array CGH was performed using Cyto Chip Focus Constitutional BAC array (Illumina Inc., San Diego, CA, USA), and the used reference was the normal human male genomic DNA (Promega Corporation, Madison, WI, USA). ..

    Article Title: De novo 8p21.3→ p23.3 Duplication With t(4;8)(q35;p21.3) Translocation Associated With Mental Retardation, Autism Spectrum Disorder, and Congenital Heart Defects: Case Report With Literature Review
    Article Snippet: .. Array Comparative Genomic Hybridization (aCGH) analysis was performed using CytoChip Focus Constitutional BAC array (Illumina Inc., U.S.) and the used reference was the normal human male genomic DNA (Promega, WI). ..

    Isolation:

    Article Title: Prenatal diagnosis of 1p34.3 interstitial microdeletion by aCGH in a fetus with jaw bone abnormalities
    Article Snippet: .. Molecular karyotype analysis by array CGH using “Illumina Cytochip Focus Constitutional array with BAC technology” was performed on DNA isolated from uncultured amniocytes according to the manufacturer’s protocol. .. The Illumina Cytochip Focus Constitutional array is a commercially available whole-genome BAC array with a median resolution of 0.5−1 Mb. (Cytochip Focus Constitutional, Illumina).

    Hybridization:

    Article Title: De novo 8p21.3→ p23.3 Duplication With t(4;8)(q35;p21.3) Translocation Associated With Mental Retardation, Autism Spectrum Disorder, and Congenital Heart Defects: Case Report With Literature Review
    Article Snippet: .. Array Comparative Genomic Hybridization (aCGH) analysis was performed using CytoChip Focus Constitutional BAC array (Illumina Inc., U.S.) and the used reference was the normal human male genomic DNA (Promega, WI). ..



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    Illumina Inc bac microarray cytochip focus constitutional
    Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human <t>BAC</t> cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC <t>microarray</t> showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.
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    Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human BAC cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC microarray showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.

    Journal: Cancer research

    Article Title: Inhibition of Cholinergic Signaling Causes Apoptosis in Human Bronchioalveolar Carcinoma

    doi: 10.1158/0008-5472.CAN-12-3190

    Figure Lengend Snippet: Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human BAC cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC microarray showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.

    Article Snippet: Human BAC tissue microarray (TMA) slides (Abnova) were deparaffinized and rehydrated as described previously ( 19 , 20 ).

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Control, Immunohistochemistry, Microarray, Isolation