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BlueGnome Limited 3379 bac clone microarray
3379 Bac Clone Microarray, supplied by BlueGnome Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

DNA Labeling:

Article Title: Proteomics studies of childhood pilocytic astrocytoma.
Article Snippet: Proteomics Studies of Childhood Pilocytic Astrocytoma Athanasios K. Anagnostopoulos, Konstantinos S. Dimas,§,r Chrissa Papathanassiou, Maria Braoudaki, Ema Anastasiadou, Konstantinos Vougas, Kalliopi Karamolegou, Harry Kontos, Neofytos Prodromou, Fotini Tzortzatou-Stathopoulou, and George Th.. Tsangaris* Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Pharmacology Division, Center of Basic Research I, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece “Genomedica” Molecular Diagnostics Laboratory, Piraeus, Greece Department of Neurosurgery, Aghia Sophia Children’s Hospital, Athens, Greece

Hybridization:

Article Title: Proteomics studies of childhood pilocytic astrocytoma.
Article Snippet: Proteomics Studies of Childhood Pilocytic Astrocytoma Athanasios K. Anagnostopoulos, Konstantinos S. Dimas,§,r Chrissa Papathanassiou, Maria Braoudaki, Ema Anastasiadou, Konstantinos Vougas, Kalliopi Karamolegou, Harry Kontos, Neofytos Prodromou, Fotini Tzortzatou-Stathopoulou, and George Th.. Tsangaris* Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Pharmacology Division, Center of Basic Research I, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece “Genomedica” Molecular Diagnostics Laboratory, Piraeus, Greece Department of Neurosurgery, Aghia Sophia Children’s Hospital, Athens, Greece

Labeling:

Article Title: Proteomics studies of childhood pilocytic astrocytoma.
Article Snippet: Proteomics Studies of Childhood Pilocytic Astrocytoma Athanasios K. Anagnostopoulos, Konstantinos S. Dimas,§,r Chrissa Papathanassiou, Maria Braoudaki, Ema Anastasiadou, Konstantinos Vougas, Kalliopi Karamolegou, Harry Kontos, Neofytos Prodromou, Fotini Tzortzatou-Stathopoulou, and George Th.. Tsangaris* Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Pharmacology Division, Center of Basic Research I, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece “Genomedica” Molecular Diagnostics Laboratory, Piraeus, Greece Department of Neurosurgery, Aghia Sophia Children’s Hospital, Athens, Greece

BAC Assay:

Article Title: Proteomics studies of childhood pilocytic astrocytoma.
Article Snippet: Proteomics Studies of Childhood Pilocytic Astrocytoma Athanasios K. Anagnostopoulos, Konstantinos S. Dimas,§,r Chrissa Papathanassiou, Maria Braoudaki, Ema Anastasiadou, Konstantinos Vougas, Kalliopi Karamolegou, Harry Kontos, Neofytos Prodromou, Fotini Tzortzatou-Stathopoulou, and George Th.. Tsangaris* Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Pharmacology Division, Center of Basic Research I, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece “Genomedica” Molecular Diagnostics Laboratory, Piraeus, Greece Department of Neurosurgery, Aghia Sophia Children’s Hospital, Athens, Greece

Microarray:

Article Title: Proteomics studies of childhood pilocytic astrocytoma.
Article Snippet: Proteomics Studies of Childhood Pilocytic Astrocytoma Athanasios K. Anagnostopoulos, Konstantinos S. Dimas,§,r Chrissa Papathanassiou, Maria Braoudaki, Ema Anastasiadou, Konstantinos Vougas, Kalliopi Karamolegou, Harry Kontos, Neofytos Prodromou, Fotini Tzortzatou-Stathopoulou, and George Th.. Tsangaris* Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Pharmacology Division, Center of Basic Research I, Biomedical Research Foundation of the Academy of Athens, Athens, Greece Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, University of Athens, Aghia Sophia Children’s Hospital, Athens, Greece “Genomedica” Molecular Diagnostics Laboratory, Piraeus, Greece Department of Neurosurgery, Aghia Sophia Children’s Hospital, Athens, Greece



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Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human BAC cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC microarray showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.

Journal: Cancer research

Article Title: Inhibition of Cholinergic Signaling Causes Apoptosis in Human Bronchioalveolar Carcinoma

doi: 10.1158/0008-5472.CAN-12-3190

Figure Lengend Snippet: Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human BAC cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC microarray showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.

Article Snippet: Human BAC tissue microarray (TMA) slides (Abnova) were deparaffinized and rehydrated as described previously ( 19 , 20 ).

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Control, Immunohistochemistry, Microarray, Isolation