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cyto chip focus constitutional bac array  (Illumina Inc)


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    Illumina Inc cyto chip focus constitutional bac array
    Cyto Chip Focus Constitutional Bac Array, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bac+array/cytochip+focus+constitutional+microarrays/pmc08777823-141-5-11
    Average 90 stars, based on 1 article reviews
    cyto chip focus constitutional bac array - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Mucinous spindle and tubular renal cell carcinoma: analysis of chromosomal aberration pattern of low-grade, high-grade, and overlapping morphologic variant with papillary renal cell carcinoma.
    Article Snippet: ☆ The study was supported by the Charles University R and by the project CZ.1.05/2.1.00/03.0076 from European ☆☆ Disclosure of conflict of interest: All authors declare ⁎ Corresponding author at: Department of Pathology, ulty and Charles University Hospital Plzen, Alej Svobody 8 Tel.. : +420377104643; fax: +420377104650.. E-mail address: hes@medima.cz (O. Hes). http://dx.doi.org/10.1016/j.anndiagpath.2015.04.004 1092-9134/© 2015 Elsevier Inc. All rights reserved.

    Article Title: Cytogenetic Investigation in 136 Consecutive Stillbirths: Does the Tissue Type Affect the Success Rate of Chromosomal Microarray Analysis and Karyotype?
    Article Snippet: Background: Chromosomal anomalies are a recognized cause of stillbirth, accounting for 6–17% of the cases.. As a diagnostic laboratory method in this setting, conventional karyotyping has two main drawbacks: the need for viable fetal cells in a dead fetus and its limited resolution as compared to alternative techniques.. Objective: To assess the effectiveness of cytogenetic analysis in stillbirths between different testing methods and different sampled tissues.

    Article Title: Routine use of array comparative genomic hybridization (aCGH) as standard approach for prenatal diagnosis of chromosomal abnormalities. Clinical experience of 1763 prenatal cases.
    Article Snippet: Objective This study aims to evaluate the diagnostic yield of comparative genomic hybridization microarrays (aCGH) and compare it with conventional karyotype analysis of standard >5-Mb resolution.. Method A total of 1763 prenatal samples were analyzed by aCGH (CytoChip Focus Constitutional microarrays, BlueGnome, Cambridge).. The diagnostic yield of chromosomal abnormalities detected by aCGH was assessed, compared with conventional karyotype analysis.

    Article Title: Have maternal or paternal ages any impact on the prenatal incidence of genomic copy number variants associated with fetal structural anomalies?
    Article Snippet: During the first four years (2012–2015) of the study, a BAC (bacterial artificial chromosome) microarray (CytoChip Focus Constitutional, BlueGenome, Illumina, San Diego, California, USA) was used with a 1 Mb resolution along the whole genome and 100 kb in 143 constitutional regions associated with pathology.

    BAC Assay:

    Article Title: Prenatal diagnosis of 1p34.3 interstitial microdeletion by aCGH in a fetus with jaw bone abnormalities
    Article Snippet: .. Molecular karyotype analysis by array CGH using “Illumina Cytochip Focus Constitutional array with BAC technology” was performed on DNA isolated from uncultured amniocytes according to the manufacturer’s protocol. .. The Illumina Cytochip Focus Constitutional array is a commercially available whole-genome BAC array with a median resolution of 0.5−1 Mb. (Cytochip Focus Constitutional, Illumina).

    Article Title: Genetic Counseling and Management: The First Study to Report NIPT Findings in a Romanian Population
    Article Snippet: .. Array CGH was performed using Cyto Chip Focus Constitutional BAC array (Illumina Inc., San Diego, CA, USA), and the used reference was the normal human male genomic DNA (Promega Corporation, Madison, WI, USA). ..

    Article Title: De novo 8p21.3→ p23.3 Duplication With t(4;8)(q35;p21.3) Translocation Associated With Mental Retardation, Autism Spectrum Disorder, and Congenital Heart Defects: Case Report With Literature Review
    Article Snippet: .. Array Comparative Genomic Hybridization (aCGH) analysis was performed using CytoChip Focus Constitutional BAC array (Illumina Inc., U.S.) and the used reference was the normal human male genomic DNA (Promega, WI). ..

    Isolation:

    Article Title: Prenatal diagnosis of 1p34.3 interstitial microdeletion by aCGH in a fetus with jaw bone abnormalities
    Article Snippet: .. Molecular karyotype analysis by array CGH using “Illumina Cytochip Focus Constitutional array with BAC technology” was performed on DNA isolated from uncultured amniocytes according to the manufacturer’s protocol. .. The Illumina Cytochip Focus Constitutional array is a commercially available whole-genome BAC array with a median resolution of 0.5−1 Mb. (Cytochip Focus Constitutional, Illumina).

    Hybridization:

    Article Title: De novo 8p21.3→ p23.3 Duplication With t(4;8)(q35;p21.3) Translocation Associated With Mental Retardation, Autism Spectrum Disorder, and Congenital Heart Defects: Case Report With Literature Review
    Article Snippet: .. Array Comparative Genomic Hybridization (aCGH) analysis was performed using CytoChip Focus Constitutional BAC array (Illumina Inc., U.S.) and the used reference was the normal human male genomic DNA (Promega, WI). ..



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    Image Search Results


    CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).

    Journal: PLoS ONE

    Article Title: An Integrated Genomic and Expression Analysis of 7q Deletion in Splenic Marginal Zone Lymphoma

    doi: 10.1371/journal.pone.0044997

    Figure Lengend Snippet: CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).

    Article Snippet: summarised the 7q deletion in SMZL detected by CGH using a chromosome-7 BAC tile-path array (17 cases) and the Agilent aCGH 244A array CGH (10 cases) from our previous studies , .

    Techniques: Expressing, Standard Deviation

    Clinical cycle of comprehensive preimplantation genetic testing. The process starts with family genetic testing in order to establish normal and disease-associated family haplotypes. Next, oocyte stimulation, retrieval, and fertilization are performed. Embryos surviving until day 5 undergo laser-assisted hatching and biopsy. Biopsy material consisting of a few trophectoderm cells is amplified using one of the WGA techniques. WGA material is further used to assess the embryonic genome, including haplotype, causative variant, aneuploidy, and CNV analyses. The disease-free embryo is subjected to embryo transfer to the uterine cavity. The whole cycle can be repeated within 24 h, with a day-6 embryo being transferred. If the genetic testing takes longer, then embryos are vitrified and thawed before being transferred. PGT—preimplantation genetic testing, WGA—whole-genome amplification, IVF—in vitro fertilization, MPS—massively parallel sequencing, CNV—copy number variation.

    Journal: International Journal of Molecular Sciences

    Article Title: Whole Genome Amplification in Preimplantation Genetic Testing in the Era of Massively Parallel Sequencing

    doi: 10.3390/ijms23094819

    Figure Lengend Snippet: Clinical cycle of comprehensive preimplantation genetic testing. The process starts with family genetic testing in order to establish normal and disease-associated family haplotypes. Next, oocyte stimulation, retrieval, and fertilization are performed. Embryos surviving until day 5 undergo laser-assisted hatching and biopsy. Biopsy material consisting of a few trophectoderm cells is amplified using one of the WGA techniques. WGA material is further used to assess the embryonic genome, including haplotype, causative variant, aneuploidy, and CNV analyses. The disease-free embryo is subjected to embryo transfer to the uterine cavity. The whole cycle can be repeated within 24 h, with a day-6 embryo being transferred. If the genetic testing takes longer, then embryos are vitrified and thawed before being transferred. PGT—preimplantation genetic testing, WGA—whole-genome amplification, IVF—in vitro fertilization, MPS—massively parallel sequencing, CNV—copy number variation.

    Article Snippet: PGT performed on trophectoderm biopsies using PicoPLEX in combination with BlueGnome’s BAC arrays (later Illumina, now discontinued) and subsequently MPS began a major revolution in the availability of human PGT and forever changed the face of clinical in vitro fertilization [ , , ].

    Techniques: Amplification, Variant Assay, Whole Genome Amplification, In Vitro, Sequencing