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zeiss axioobserver z1  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss axioobserver z1
    Figure 1. Matrigel Branching Assays using Endometrial Tissue Conditioned Media. A, B, and C: Media only control. Cells treated with conditioned media endometrium supplying the closest to mean litter weight fetus (CTMLW) (D, E, and F) and lightest fetus (G, H, and I) at gestational day (GD) 45. Cells treated with conditioned media from endometrium supplying the CTMLW (J, K, and L) or lightest fetus (M, N, and O) at GD60. All conditioned media collected after 18 hours of tissue explant culture. The CTMLW and lightest fetuses within GD were from the same litter. Scale bars represent 10 μM. Representative images taken using the Zeiss <t>AxioObserver</t> <t>Z1</t> at ×5 magnification at 2, 6, and 10 hours post-seeding of the endothelial cells.
    Zeiss Axioobserver Z1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/Shutter+FL%2C+internal+for+stands+Axio+Observer+3+%2F+5+%2F+7+%2F+D1+%2F+Z1/pm31093645-168-5-5
    Average 97 stars, based on 568 article reviews
    zeiss axioobserver z1 - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Novel relationships between porcine fetal size, sex, and endometrial angiogenesis†."

    Article Title: Novel relationships between porcine fetal size, sex, and endometrial angiogenesis†.

    Journal: Biology of reproduction

    doi: 10.1093/biolre/ioz068

    Figure 1. Matrigel Branching Assays using Endometrial Tissue Conditioned Media. A, B, and C: Media only control. Cells treated with conditioned media endometrium supplying the closest to mean litter weight fetus (CTMLW) (D, E, and F) and lightest fetus (G, H, and I) at gestational day (GD) 45. Cells treated with conditioned media from endometrium supplying the CTMLW (J, K, and L) or lightest fetus (M, N, and O) at GD60. All conditioned media collected after 18 hours of tissue explant culture. The CTMLW and lightest fetuses within GD were from the same litter. Scale bars represent 10 μM. Representative images taken using the Zeiss AxioObserver Z1 at ×5 magnification at 2, 6, and 10 hours post-seeding of the endothelial cells.
    Figure Legend Snippet: Figure 1. Matrigel Branching Assays using Endometrial Tissue Conditioned Media. A, B, and C: Media only control. Cells treated with conditioned media endometrium supplying the closest to mean litter weight fetus (CTMLW) (D, E, and F) and lightest fetus (G, H, and I) at gestational day (GD) 45. Cells treated with conditioned media from endometrium supplying the CTMLW (J, K, and L) or lightest fetus (M, N, and O) at GD60. All conditioned media collected after 18 hours of tissue explant culture. The CTMLW and lightest fetuses within GD were from the same litter. Scale bars represent 10 μM. Representative images taken using the Zeiss AxioObserver Z1 at ×5 magnification at 2, 6, and 10 hours post-seeding of the endothelial cells.

    Techniques Used: Control

    Related Articles

    other:

    Article Title: Human cell F-actin density differentially influences trogocytosis and phagocytosis by Entamoeba histolytica
    Article Snippet: Images were collected using a 40X objective on an Axio Observer Z1/7 (Zeiss LSM 980) with Airyscan2.

    Article Title: Human cell F-actin density differentially influences trogocytosis and phagocytosis by Entamoeba histolytica
    Article Snippet: Images were collected using a 63X objective on an Axio Observer Z1/7 (Zeiss LSM 980) with Airyscan2.

    Microscopy:

    Article Title: ER-Lysosome Cholesterol Exchange Regulates Lysosomal Motility Through mTOR-Dependent LAMTOR1 Phosphorylation
    Article Snippet: .. Images from ARL8B and BORCS5 siRNA experiments were captured using an Axiovert 200 M microscope equipped with an AxioCam705 camera; images from HA-LAMTOR1-LLL-AAA experiments were captured using a Zeiss Axio Observer.Z1/7 Airyscan microscope. ..

    Immunofluorescence:

    Article Title: Modeling Clostridioides difficile toxin pathogenesis and antiserum protection in an immunocompetent intestine-on-chip platform.
    Article Snippet: .. Immunofluorescence z-stack images were obtained with an Axio Observer 5, an ApoTome for optical sectioning, and a 20 × objective, NA 0.8, all from Carl Zeiss Microscopy GmbH (Jena, Germany). ..

    Article Title: Modeling Clostridioides difficile toxin pathogenesis and antiserum protection in an immunocompetent intestine-on-chip platform
    Article Snippet: After 3 additional washing steps in washing solution, the membranes were mounted in fluorescence mounting medium (Sigma, Merck, Darmstadt, Germany) on glass slides (VWR International GmbH, Darmstadt, Germany) and covered by a glass coverslip (VWR International GmbH, Darmstadt, Germany). .. Immunofluorescence z-stack images were obtained with an Axio Observer 5, an ApoTome for optical sectioning, and a 20 × objective, NA 0.8, all from Carl Zeiss Microscopy GmbH (Jena, Germany). ..

    Inverted Microscopy:

    Article Title: Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy.
    Article Snippet: .. The equipment and settings for the presented protocol used were as follows: a. Zeiss Axio Observer.Z1/7 inverted microscope with LSM 980 confocal scanner. b. ..

    H&E Stain:

    Article Title: Immunomodulatory hydrogel reprograms IL-17/NF-κB signaling to drive regeneration in diabetic wounds
    Article Snippet: .. Hematoxylin and eosin (H&E) staining was performed to observe immune cell morphology using an Axio Observer 7 (Carl Zeiss, Germany) according to standard protocols [ ]. .. For CD86 and CD206 detection, slices were co-incubated overnight at 4 °C with rat anti-CD86 antibody (1:1000; Abcam, UK) and rabbit anti-CD206 antibody (1:1000; Abcam, UK).

    Immune Cell:

    Article Title: Immunomodulatory hydrogel reprograms IL-17/NF-κB signaling to drive regeneration in diabetic wounds
    Article Snippet: .. Hematoxylin and eosin (H&E) staining was performed to observe immune cell morphology using an Axio Observer 7 (Carl Zeiss, Germany) according to standard protocols [ ]. .. For CD86 and CD206 detection, slices were co-incubated overnight at 4 °C with rat anti-CD86 antibody (1:1000; Abcam, UK) and rabbit anti-CD206 antibody (1:1000; Abcam, UK).

    Morphology:

    Article Title: Immunomodulatory hydrogel reprograms IL-17/NF-κB signaling to drive regeneration in diabetic wounds
    Article Snippet: .. Hematoxylin and eosin (H&E) staining was performed to observe immune cell morphology using an Axio Observer 7 (Carl Zeiss, Germany) according to standard protocols [ ]. .. For CD86 and CD206 detection, slices were co-incubated overnight at 4 °C with rat anti-CD86 antibody (1:1000; Abcam, UK) and rabbit anti-CD206 antibody (1:1000; Abcam, UK).

    Software:

    Article Title: Customizable FDM-based zebrafish larvae mold for live imaging.
    Article Snippet: Imaging of the mounted larvae was performed using a Marianas CSU-W1 (Intelligent Imaging Innovations, 3I) spinning disk confocal microscope equipped with a Hamamatsu sCMOS Orca Flash 4.0 camera, an environmental chamber, and control (OkoLab), set to 28.5 °C. .. The system was operated via Zeiss Axio Observer 7 frame and Slidebook 6 software. .. Overnight time-lapse imaging used a 25x/0.8 multi-immersion objective (LD LCI Plan-ApoC, Zeiss, 420852-9871-799) at 20-minute intervals, acquiring an 82 μm stack with a 2 μm step size.



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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison