afm system (Bruker Corporation)

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Bruker Corporation afm system

Representative images indicating the regions of interest subjected to elasticity measurements via <t>AFM.</t> Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with <t>the</t> <t>inverted</t> AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.

Afm System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/axioobserver+d1+fluorescence+microscope/DNP-NMR/pmc08070533-166-2-6
Average 96 stars, based on 1 article reviews

afm system - by Bioz Stars, 2026-10

96/100 stars

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1) Product Images from "Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology"

Article Title: Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms22083958

Representative images indicating the regions of interest subjected to elasticity measurements via AFM. Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.

Figure Legend Snippet: Representative images indicating the regions of interest subjected to elasticity measurements via AFM. Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.

Techniques Used: Derivative Assay, Control, Injection, Light Microscopy

Irradiation:

Article Title: Atomic Structure of Phosphorylated Nanocellulose Revealed by Dynamic Nuclear Polarization-Enhanced Solid-State NMR
Article Snippet: .. All the experiments were performed on a Bruker Avance III 400 MHz ( 1 H resonance) DNP-NMR spectrometer equipped with a 263 GHz gyrotron for microwave irradiation at 105 K. Chemical shift referencing was performed indirectly at room temperature using the CH signal of adamantane for 13 C at 37.8 ppm and that of hydroxyapatite for 31 P at 2.8 ppm., It is important to note that 13 C and 31 P chemical shifts measured under low-temperature DNP conditions can differ from room-temperature values in a site-dependent manner. .. All the experiments were performed on a Bruker Avance III 400 MHz ( 1 H resonance) DNP-NMR spectrometer equipped with a 263 GHz gyrotron for microwave irradiation at 105 K. Chemical shift referencing was performed indirectly at room temperature using the CH signal of adamantane for 13 C at 37.8 ppm and that of hydroxyapatite for 31 P at 2.8 ppm., It is important to note that 13 C and 31 P chemical shifts measured under low-temperature DNP conditions can differ from room-temperature values in a site-dependent manner.

Article Title: Atomic Structure of Phosphorylated Nanocellulose Revealed by Dynamic Nuclear Polarization-Enhanced Solid-State NMR.
Article Snippet: Atomic-level details of nanocellulose structure, particularly at its surface, remain difficult to resolve and are therefore poorly understood.. Here we apply dynamic nuclear polarization (DNP)-enhanced solid-state NMR to directly probe the surface chemistry of phosphorylated cellulose nanofibers.. Multidimensional 13C−13C and 31P−13C correlation experiments reveal both monoand diphosphate substitution, with C2 and C6 identified as the preferred sites.

Nuclear Magnetic Resonance:

Article Title: A one-pot organocatalytic process for the synthesis of cyclic carbonates from CO 2 and alkenes using cumene hydroperoxide as a green oxidant
Article Snippet: .. The NMR analyses were performed on a Bruker Ascend 600 MHz spectrometer using a quantification protocol (8 scans, delay D1 35 s, acquisition time AQ 5 s). were used to determine the amount in moles of each species “i” at the end of the reaction ( n i, t ), the conversion ( X ), yield ( Y ), mole balance (MB), selectivity ( S ) and CHP efficiency ( E CHP ), respectively. .. The NMR analyses were performed on a Bruker Ascend 600 MHz spectrometer using a quantification protocol (8 scans, delay D1 35 s, acquisition time AQ 5 s).

Article Title: Ultra-high surface area silica material and its application for selective N -formylation using a CO 2 surrogate
Article Snippet: .. Solid State 29 Si CP/MAS NMR measurements were performed using a Bruker Avance III WB 600 MHz DNP NMR spectrometer operating at 14.1 T, employing a 3.2 mm Triple Resonance HXY probe. .. Solid State 29 Si CP/MAS NMR measurements were performed using a Bruker Avance III WB 600 MHz DNP NMR spectrometer operating at 14.1 T, employing a 3.2 mm Triple Resonance HXY probe.

Article Title: Method for producing a poly(anthranilamide), poly(anthranilamide) and use thereof
Article Snippet: .. The number-average molar mass (Mn) of the resulting poly(anthranilamide) was determined by means of 1H NMR spectroscopy (from Bruker, AV III HD 600, 600 MHz; pulse sequence zg30, delay time d1: 10 s, 64 scans). ..

Fourier Transform Infrared Spectroscopy:

Article Title: Preparation of deuterium-labeled κ-carrageenan and application in degradation evaluation for tissue engineering scaffolds based on κ-carrageenan.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Proton NMR:

Article Title: Determination of modification degree of therapeutic proteins using 1H-NMR spectroscopy
Article Snippet: .. Q-NMR Acquisition Parameters The proton NMR spectra can be acquired on a 600 MHz AVANCE BRUKER spectrometer as follows: Spectrometer Frequency: 600 MHz Pulse Program: zgesgp; Temperature (° C.): 25±5; Number of scans: 3000 SW (ppm): >14 (approximately +10 to −4); Receiver Gain: optimize D1 (sec): 1 Q-NMR Processing Parameters The proton-NMR spectra can be processed using BRUKER Topspin software V3.5 as follows: Window functions: EM (Line broadening: 0.3 Hz); The following commands can be executed: ft: Fourier transform apk: automatic phase correction abs: automatic baseline correction Axis calibration: Spectrum reference frequency can be matched with a DSS SR value after calibration at 0.00 ppm of the methyl signal. ..

Article Title: Determination of modification degree of therapeutic proteins using 1H-NMR spectroscopy
Article Snippet: .. Acquisition Parameters The proton NMR spectra was acquired on a 600 MHz AVANCE BRUKER spectrometer as follow: Spectrometer Frequency: 600 MHz Pulse Program: zgesgp; Temperature (° C.): 25±5; Number of scans: 3000 SW (ppm): >14 (approximately +10 to −4); Receiver Gain: optimize D1 (sec): 1 Processing Parameters The proton-NMR spectra was processed using BRUKER Topspin software V3.5 as follows: Window functions: EM (Line broadening: 0.3 Hz); The following commands were executed: ft: Fourier transform apk: automatic phase correction abs: automatic baseline correction Axis calibration: Spectrum reference frequency was matched with a DSS SR value after calibration at 0.00 ppm of the methyl signal. ..

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afm system (Bruker Corporation)

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1) Product Images from "Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology"

Related Articles

Irradiation:

Nuclear Magnetic Resonance:

Fourier Transform Infrared Spectroscopy:

Proton NMR:

Software:

Western Blot:

Spectroscopy:

Sequencing:

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