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zeiss inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss inverted fluorescence microscope
    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron <t>microscope</t> images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead <t>fluorescence</t> images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Zeiss Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+fluorescence+microscope/Inverted+microscope+Axio+Observer+7/pmc13014975-149-14-18
    Average 98 stars, based on 1003 article reviews
    zeiss inverted fluorescence microscope - by Bioz Stars, 2026-11
    98/100 stars

    Images

    1) Product Images from "Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions"

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.103015

    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture

    Related Articles

    Imaging:

    Article Title: A silent intergenic locus for efficient and versatile transgene expression in Plasmodium falciparum
    Article Snippet: This work was supported by Radboud University Medical Centre (Radboudumc Individual PhD for Masters round 2021 to LJA; Radboudumc Individual PhD for Masters round 2021 to ETB) and the Dutch Research Council (NWO, grant numbers: OCENW.M.21.087 to TWAK; VI.Veni.232.160 and OCENW.XS25.2.028 to LEdV). .. The biosafety level 2 live imaging facility was established with financial support for acquisition of the Zeiss Axio Observer 7 Inverted LED microscope from Dutch Research Council (NWO, grant number: OCENW.M.21.087 to TWAK) and for lab reconstruction from the Radboudumc department of Medical Microbiology. ..

    Microscopy:

    Article Title: A silent intergenic locus for efficient and versatile transgene expression in Plasmodium falciparum
    Article Snippet: This work was supported by Radboud University Medical Centre (Radboudumc Individual PhD for Masters round 2021 to LJA; Radboudumc Individual PhD for Masters round 2021 to ETB) and the Dutch Research Council (NWO, grant numbers: OCENW.M.21.087 to TWAK; VI.Veni.232.160 and OCENW.XS25.2.028 to LEdV). .. The biosafety level 2 live imaging facility was established with financial support for acquisition of the Zeiss Axio Observer 7 Inverted LED microscope from Dutch Research Council (NWO, grant number: OCENW.M.21.087 to TWAK) and for lab reconstruction from the Radboudumc department of Medical Microbiology. ..

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Mechanosensation of rice root border cells activates serotonin-mediated pre-invasion behaviors in Meloidogyne graminicola via DEG/ENaC channels.
    Article Snippet: .. The samples were heat-killed and immediately observed under the inverted fluorescence microscope Zeiss Axio Observer 7 (Carl Zeiss AG, Oberkochen, Germany) using a suitable filter set for Cy3 detection [20]. .. Fluorescence intensity within the nematode esophagus was quantified using ImageJ software.

    Article Title: Sesamin Attenuates Interleukin-1β-Induced Inflammation in Human Synovial Cells: Involvement of the Nrf2/HO‑1 Pathway and NLRP3 Expression
    Article Snippet: .. Moreover, fluorescent images were acquired using a Zeiss Axio Observer 7 inverted fluorescence microscope equipped with Plan-Apochromat objective lenses and a Zeiss Axiocam 506 mono digital camera. .. The ROS-dependent fluorescence was detected using a Zeiss Filter Set 38 (excitation: 470/40 nm; emission: 525/50 nm).

    Article Title: Identification of a CREB-regulated transcription coactivator (CRTC) in Crassostrea gigas and its role in regulating melanin production.
    Article Snippet: Shell pigmentation is a critical economic trait that influences the commercial value of aquacultural oyster, with coloration primarily governed by melanin deposition.. In melanin biosynthesis, the cAMP-CREB-MITF signaling axis serves as the core regulatory pathway, wherein the CREB-regulated transcription coactivator (CRTC) functions as an important coactivator for CREB.. In this study, we identified and functionally characterized the CRTC homologous gene CgCRTC from the Pacific oyster (Crassostrea gigas).

    Fluorescence:

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Mechanosensation of rice root border cells activates serotonin-mediated pre-invasion behaviors in Meloidogyne graminicola via DEG/ENaC channels.
    Article Snippet: .. The samples were heat-killed and immediately observed under the inverted fluorescence microscope Zeiss Axio Observer 7 (Carl Zeiss AG, Oberkochen, Germany) using a suitable filter set for Cy3 detection [20]. .. Fluorescence intensity within the nematode esophagus was quantified using ImageJ software.

    Article Title: Sesamin Attenuates Interleukin-1β-Induced Inflammation in Human Synovial Cells: Involvement of the Nrf2/HO‑1 Pathway and NLRP3 Expression
    Article Snippet: .. Moreover, fluorescent images were acquired using a Zeiss Axio Observer 7 inverted fluorescence microscope equipped with Plan-Apochromat objective lenses and a Zeiss Axiocam 506 mono digital camera. .. The ROS-dependent fluorescence was detected using a Zeiss Filter Set 38 (excitation: 470/40 nm; emission: 525/50 nm).

    Article Title: Identification of a CREB-regulated transcription coactivator (CRTC) in Crassostrea gigas and its role in regulating melanin production.
    Article Snippet: Shell pigmentation is a critical economic trait that influences the commercial value of aquacultural oyster, with coloration primarily governed by melanin deposition.. In melanin biosynthesis, the cAMP-CREB-MITF signaling axis serves as the core regulatory pathway, wherein the CREB-regulated transcription coactivator (CRTC) functions as an important coactivator for CREB.. In this study, we identified and functionally characterized the CRTC homologous gene CgCRTC from the Pacific oyster (Crassostrea gigas).

    Confocal:

    Article Title: Loss of myosin VI leads to age-dependent changes in the maintenance of heart ventricle mitochondria
    Article Snippet: .. Images were collected using Confocal Laser Scanning Microscope with Airyscanning Zeiss LSM800, Inverted Axio Observer.Z1/7 equipped with Plan-Apochromat 40x/1.4 Oil DIC (UV) VIS-IR M27 objective. ..

    Laser-Scanning Microscopy:

    Article Title: Loss of myosin VI leads to age-dependent changes in the maintenance of heart ventricle mitochondria
    Article Snippet: .. Images were collected using Confocal Laser Scanning Microscope with Airyscanning Zeiss LSM800, Inverted Axio Observer.Z1/7 equipped with Plan-Apochromat 40x/1.4 Oil DIC (UV) VIS-IR M27 objective. ..

    Inverted Microscopy:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.

    Software:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.



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