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Arraystar inc microarray analysis of human circrnas
a Volcano plots showed differential expression of <t>circRNAs</t> detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis
Microarray Analysis Of Human Circrnas, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis"

Article Title: Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis

Journal: Cell Death & Disease

doi: 10.1038/s41419-019-1978-2

a Volcano plots showed differential expression of circRNAs detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis
Figure Legend Snippet: a Volcano plots showed differential expression of circRNAs detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis

Techniques Used: Quantitative Proteomics, Microarray, Control, Quantitative RT-PCR, Expressing, Fluorescence, Flow Cytometry, Western Blot

Related Articles

Microarray:

Article Title: Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
Article Snippet: .. The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients ( n =3) and age-matched normal donors (NA; n =3) were analyzed using the Arraystar human circRNAs microarray. ..

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention
Article Snippet: .. This analytical endeavor employs the Arraystar human circRNAs microarray to probe five samples of each tissue type, revealing a plethora of circRNAs that undergo significant upregulation or downregulation in IAs [ ]. ..

Article Title: Identification of hsa_circ_0018905 as a New Potential Biomarker for Multiple Sclerosis.
Article Snippet: .. Here, we explore the panorama of circRNA expression in PBMCs purified from five newly diagnosed MS patients and five healthy controls (HCs) using the Arraystar Human circRNAs microarray. ..

Article Title: Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis
Article Snippet: .. Identification of differentially expressed circRNAs was performed by overlapping microarray analysis of human circRNAs (Arraystar, CA, USA) and microarray dataset (GSE67566) obtained from Gene Expression Omnibus (GEO) database. ..

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention
Article Snippet: .. Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed. ..

Article Title: Role of microRNAs, circRNAs and long noncoding RNAs in acute myeloid leukemia
Article Snippet: .. Chen et al. [ ] reported that circANAPC7 was significantly upregulated in AML and used an Arraystar human circRNAs microarray and bioinformatics analysis to predict when ANAPC7 might bind miR-181 family to participate in AML pathogenesis. ..

Article Title: The potential role of hsa_circ_0005505 in the rupture of human intracranial aneurysm
Article Snippet: .. Results The differentially expressed circular RNAs between un-ruptured intracranial aneurysms (n = 5) and ruptured intracranial aneurysms (n = 5) were analyzed with the Arraystar human circRNAs microarray and total of 13175 circular RNAs were detected. ..

Expressing:

Article Title: Identification of hsa_circ_0018905 as a New Potential Biomarker for Multiple Sclerosis.
Article Snippet: .. Here, we explore the panorama of circRNA expression in PBMCs purified from five newly diagnosed MS patients and five healthy controls (HCs) using the Arraystar Human circRNAs microarray. ..

Purification:

Article Title: Identification of hsa_circ_0018905 as a New Potential Biomarker for Multiple Sclerosis.
Article Snippet: .. Here, we explore the panorama of circRNA expression in PBMCs purified from five newly diagnosed MS patients and five healthy controls (HCs) using the Arraystar Human circRNAs microarray. ..

Gene Expression:

Article Title: Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis
Article Snippet: .. Identification of differentially expressed circRNAs was performed by overlapping microarray analysis of human circRNAs (Arraystar, CA, USA) and microarray dataset (GSE67566) obtained from Gene Expression Omnibus (GEO) database. ..

other:

Article Title: Hsa_circRNA_102002 facilitates metastasis of papillary thyroid cancer through regulating miR-488-3p/HAS2 axis.
Article Snippet: Microarray of circRNAs was performed in BGI.Tech (Beijing, China) and analyzed according to the Arraystar.



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Image Search Results


Detailed information of the datasets selected in this study.

Journal: Annals of Medicine

Article Title: Reveal the potential molecular mechanism of circRNA regulating immune-related mRNA through sponge miRNA in the occurrence and immune regulation of papillary thyroid cancer

doi: 10.1080/07853890.2023.2244515

Figure Lengend Snippet: Detailed information of the datasets selected in this study.

Article Snippet: GSE93522 , Nianchun Peng , GPL19978 Agilent-069978 Arraystar Human CircRNA microarray V1 , 6 : 6 , 2017 , Tissue , circRNA.

Techniques: Microarray