microarray analysis of human circrnas (Arraystar inc)
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Microarray Analysis Of Human Circrnas, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arraystar+human+circrna+microarray/human+circrnas+microarray/pmc06776655-23-13-14
Average 90 stars, based on 1 article reviews
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1) Product Images from "Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis"
Article Title: Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis
Journal: Cell Death & Disease
doi: 10.1038/s41419-019-1978-2
Figure Legend Snippet: a Volcano plots showed differential expression of circRNAs detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis
Techniques Used: Quantitative Proteomics, Microarray, Control, Quantitative RT-PCR, Expressing, Fluorescence, Flow Cytometry, Western Blot
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