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tissue arrayer device beecher instruments  (Beecher Instruments Inc)

 
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    Beecher Instruments Inc tissue arrayer device beecher instruments
    Tissue Arrayer Device Beecher Instruments, supplied by Beecher Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arraymate+device/tissue+array+device/pmc02775867-24-21-24
    Average 90 stars, based on 1 article reviews
    tissue arrayer device beecher instruments - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Carboxypeptidase A4 accumulation is associated with an aggressive phenotype and poor prognosis in triple-negative breast cancer
    Article Snippet: .. Breast cancer tissue cores (2.0-mm diameter per tumor) were punched out from the representative areas near the invasive front and transferred into the paired recipient paraffin block using a tissue array instrument (Beecher Instruments, Inc., Silver Spring, MD, USA). ..

    Article Title: Downregulation of LIMK1-ADF/cofilin by DADS inhibits the migration and invasion of colon cancer.
    Article Snippet: Leica paraffin (Leica Histowax, Leica Biosystems, Germany) and beeswax (Hualing, Shanghai, China) were mixed in aratio of 7:3 to prepare a recipient paraffin block of size 2.2 × 3.5 × 1 cm3 and design tissue arrays of 5 × 10 points. .. A tissue microarray instrument (Beecher Instruments Inc., WI, USA) was used to punch and produce a blank wax block with holes. .. The tissue paraffin block was placed in a water bath at 39–42 °C for 3 min, and a 2-mm needle (Beecher Instruments Inc., WI, USA) was used to select lesions.

    Microarray:

    Article Title: Prognostic significance of thymidylate synthase in postoperative non-small cell lung cancer patients
    Article Snippet: .. The tissue was sampled using a tissue microarray instrument (Beecher Instruments Inc., Sun Prairie, WI, USA). ..

    Article Title: Oral Microbiome and CPT1A Function in Fatty Acid Metabolism in Oral Cancer
    Article Snippet: The membranes were blocked with 5% skim milk and probed with a primary antibody against CPT1A (ab128568, Abcam, Cambridge, UK) and a secondary horseradish peroxidase-conjugated anti-mouse antibody (A90–116P; Bethyl Laboratories, Montgomery, TX, USA). .. A tissue microarray (TMA) was constructed using paraffin-embedded blocks from six tumor samples with a tissue array device (Beecher Instruments Inc., Sun Prairie, WI, USA). ..

    Article Title: Tumour Infiltrating Lymphocytes (TILs) and immune composition in breast cancer patients from Kenya: Spatial distributions and associations with risk factors and tumour characteristics.
    Article Snippet: Background The immune landscape of breast cancer (BC) in patients from Sub Saharan Africa is understudied.. Our aims were to describe the distribution of Tumour Infiltrating Lymphocytes (TILs) within the intratumoural stroma (sTILs) and the leading/invasive edge stroma (LE-TILs), and to evaluate TILs across BC subtypes with established risk factors and clinical characteristics in Kenyan women.. Methods Visual quantification of sTILs and LE-TILs were performed on Haematoxylin and eosin -stained pathologically confirmed BC cases based on the International TIL working group guidelines.

    Article Title: Downregulation of LIMK1-ADF/cofilin by DADS inhibits the migration and invasion of colon cancer.
    Article Snippet: Leica paraffin (Leica Histowax, Leica Biosystems, Germany) and beeswax (Hualing, Shanghai, China) were mixed in aratio of 7:3 to prepare a recipient paraffin block of size 2.2 × 3.5 × 1 cm3 and design tissue arrays of 5 × 10 points. .. A tissue microarray instrument (Beecher Instruments Inc., WI, USA) was used to punch and produce a blank wax block with holes. .. The tissue paraffin block was placed in a water bath at 39–42 °C for 3 min, and a 2-mm needle (Beecher Instruments Inc., WI, USA) was used to select lesions.

    Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes epithelial–mesenchymal transition in diffuse-type gastric cancer via cAMP response element-binding protein pathway, showing poor prognosis
    Article Snippet: .. Tissue microarray (TMA) blocks of 289 gastric cancer cases who underwent curative gastrectomy were constructed using a tissue array device (Beecher Instruments Inc.) from surgical specimens collected between May 2006 and March 2012 at Seoul National University Bundang Hospital (SNUBH). ..

    Construct:

    Article Title: Oral Microbiome and CPT1A Function in Fatty Acid Metabolism in Oral Cancer
    Article Snippet: The membranes were blocked with 5% skim milk and probed with a primary antibody against CPT1A (ab128568, Abcam, Cambridge, UK) and a secondary horseradish peroxidase-conjugated anti-mouse antibody (A90–116P; Bethyl Laboratories, Montgomery, TX, USA). .. A tissue microarray (TMA) was constructed using paraffin-embedded blocks from six tumor samples with a tissue array device (Beecher Instruments Inc., Sun Prairie, WI, USA). ..

    Article Title: KMT2C mutations in diffuse type gastric adenocarcinoma promote epithelial-to-mesenchymal transition
    Article Snippet: Stained cells were visualized using an inverted confocal microscope and image was processed using Imaris 7.6. .. Tumor tissue microarrays (TMAs) were constructed using tumors from patients with diffuse type GA who underwent radical gastrectomy or esophagogastrectomy with potentially curative intent (R0 and R1) from May 2006 to March 2012 at Seoul National University Bundang Hospital (SNUBH; South Korea) using a tissue array device (Beecher Instruments Inc., Sun Prairie, WI). ..

    Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes epithelial–mesenchymal transition in diffuse-type gastric cancer via cAMP response element-binding protein pathway, showing poor prognosis
    Article Snippet: .. Tissue microarray (TMA) blocks of 289 gastric cancer cases who underwent curative gastrectomy were constructed using a tissue array device (Beecher Instruments Inc.) from surgical specimens collected between May 2006 and March 2012 at Seoul National University Bundang Hospital (SNUBH). ..



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    (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the <t>ArrayMate™</t> Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.
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    Image Search Results


    (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.

    Journal: PLoS ONE

    Article Title: Fast DNA Serotyping and Antimicrobial Resistance Gene Determination of Salmonella enterica with an Oligonucleotide Microarray-Based Assay

    doi: 10.1371/journal.pone.0046489

    Figure Lengend Snippet: (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.

    Article Snippet: The visualization was achieved by adding 100 µl of peroxidase substrate D1 to the ArrayStrips, and signals were detected with the ArrayMate device (Alere Technologies, Jena, Germany) ( ).

    Techniques: Multiplex Assay, Amplification, Labeling, Hybridization, Software