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manual arrayer  (Pathology Devices Inc)

 
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    Pathology Devices Inc manual arrayer
    Manual Arrayer, supplied by Pathology Devices Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arraymate+device/manual+arrayer/pmc04804727-152-24-25
    Average 90 stars, based on 1 article reviews
    manual arrayer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Using Pharmacokinetic Profiles and Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Early Signaling Changes Following Integrin-Linked Kinase Inhibition in an In Vivo Model of Cancer
    Article Snippet: TMAs were constructed by removing two 0.6-mm cores from selected formalin-fixed, paraffin-embedded tissue blocks and transferred to a recipient paraffin block using a manual arrayer (Pathology Devices Inc.; Westminster, MD).

    Article Title: Toward a molecular pathologic classification of urothelial carcinoma.
    Article Snippet: Tissue Microarrays and IHC TMA blocks were constructed from 1.0 mm punches of formalin-fixed paraffin-embedded urothelial carcinoma specimens using a manual arrayer (Pathology Devices, Inc., Westminster, MD).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Using Pharmacokinetic Profiles and Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Early Signaling Changes Following Integrin-Linked Kinase Inhibition in an In Vivo Model of Cancer
    Article Snippet: TMAs were constructed by removing two 0.6-mm cores from selected formalin-fixed, paraffin-embedded tissue blocks and transferred to a recipient paraffin block using a manual arrayer (Pathology Devices Inc.; Westminster, MD).

    Article Title: Toward a molecular pathologic classification of urothelial carcinoma.
    Article Snippet: Tissue Microarrays and IHC TMA blocks were constructed from 1.0 mm punches of formalin-fixed paraffin-embedded urothelial carcinoma specimens using a manual arrayer (Pathology Devices, Inc., Westminster, MD).

    Blocking Assay:

    Article Title: Using Pharmacokinetic Profiles and Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Early Signaling Changes Following Integrin-Linked Kinase Inhibition in an In Vivo Model of Cancer
    Article Snippet: TMAs were constructed by removing two 0.6-mm cores from selected formalin-fixed, paraffin-embedded tissue blocks and transferred to a recipient paraffin block using a manual arrayer (Pathology Devices Inc.; Westminster, MD).

    Article Title: Toward a molecular pathologic classification of urothelial carcinoma.
    Article Snippet: Tissue Microarrays and IHC TMA blocks were constructed from 1.0 mm punches of formalin-fixed paraffin-embedded urothelial carcinoma specimens using a manual arrayer (Pathology Devices, Inc., Westminster, MD).

    Immunohistochemistry:

    Article Title: Using Pharmacokinetic Profiles and Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Early Signaling Changes Following Integrin-Linked Kinase Inhibition in an In Vivo Model of Cancer
    Article Snippet: TMAs were constructed by removing two 0.6-mm cores from selected formalin-fixed, paraffin-embedded tissue blocks and transferred to a recipient paraffin block using a manual arrayer (Pathology Devices Inc.; Westminster, MD).

    Article Title: Toward a molecular pathologic classification of urothelial carcinoma.
    Article Snippet: Tissue Microarrays and IHC TMA blocks were constructed from 1.0 mm punches of formalin-fixed paraffin-embedded urothelial carcinoma specimens using a manual arrayer (Pathology Devices, Inc., Westminster, MD).



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    (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the <t>ArrayMate™</t> Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.
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    (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the <t>ArrayMate™</t> Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.
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    Image Search Results


    (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.

    Journal: PLoS ONE

    Article Title: Fast DNA Serotyping and Antimicrobial Resistance Gene Determination of Salmonella enterica with an Oligonucleotide Microarray-Based Assay

    doi: 10.1371/journal.pone.0046489

    Figure Lengend Snippet: (a) Linear Multiplex Amplification starting from clonal RNA free genomic DNA, extracted DNA is internally labeled with biotin (Label [L]) and amplified in a linear multiplex PCR reaction; (b) Hybridization: the biotin labeled, single-stranded DNA product hybridizes specifically under stringent conditions to the corresponding probes. The resulting duplex is detected using a horse-radish peroxidase (Enzyme [E]) – streptavidin conjugate, which converts the substrate (Seramun green [S]) into a colored local precipitate. (c) Detection: the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) enables the visualization and subsequent automated analysis of the array image. The presence of a dark precipitated spot indicates successful hybridization; (d) Analysis: the assay specific software analysis script, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines with an assay specific algorithm which genes/alleles are present in the sample. (e) Genotype analysis: the PatternMatching software supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03) is comparing the resulting pattern with a local database including 132 reference serovars previously sero- and genotyped, finally a report is given to which serovar the sample strain belongs with regard to the Kauffman-White Scheme.

    Article Snippet: The visualization was achieved by adding 100 µl of peroxidase substrate D1 to the ArrayStrips, and signals were detected with the ArrayMate device (Alere Technologies, Jena, Germany) ( ).

    Techniques: Multiplex Assay, Amplification, Labeling, Hybridization, Software