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tnt ® coupled reticulocyte lysate system kit  (Promega)

 
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    Structured Review

    Promega tnt ® coupled reticulocyte lysate system kit
    Tnt ® Coupled Reticulocyte Lysate System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arrayjet+printing+buffer/rabbit+reticulocyte+lysate/pmc08831226-46-145-150
    Average 90 stars, based on 1 article reviews
    tnt ® coupled reticulocyte lysate system kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
    Article Snippet: .. Measurement of intracellular Ca2+ levels, [Ca2+]i Three double-stranded RNA (dsRNA) specifically targeting the transient receptor potential canonical (TRPC) Hl-16258 and Hl-21915 and the piezo-type mechanosensitive ion channel component 2 (PIEZ2) Hl-13522 were designed based on their coding sequences. dsRNAs were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI, USA). .. In addition, a non-targeting dsRNA, which is specific to enhanced green fluorescent protein (EGFP), was used as a negative control. dsRNA quality was verified after annealing by gel electrophoresis. dsRNA sequences for Hl-21915, Hl-16258, Hl-13522 and EGFP are as shown in SI Appendix, Dataset S1 W. Concentrations of synthesized dsRNA were verified with a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). dsRNAs were then diluted to 600 ng/μL in 150 mM RNasefree saline solution (RFSS).

    Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
    Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

    Article Title: Carrier-freemRNA vaccine induces robust immunity against SARS-CoV-2 in mice and non-human primates without systemic reactogenicity
    Article Snippet: .. The latter used in vitro transcribed RNA of the N protein-encoding gene (at 100, 101, 102, 103, 104, 106, and 108 copies/reaction); this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). ..

    Negative Control:

    Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
    Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

    Saline:

    Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
    Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

    In Vitro:

    Article Title: Carrier-freemRNA vaccine induces robust immunity against SARS-CoV-2 in mice and non-human primates without systemic reactogenicity
    Article Snippet: .. The latter used in vitro transcribed RNA of the N protein-encoding gene (at 100, 101, 102, 103, 104, 106, and 108 copies/reaction); this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). ..

    Article Title: Supporting Information for SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23
    Article Snippet: .. For in vitro transcription, we used a Promega T7 RNA polymerase kit. .. Transcription reactions in 20 L used Transcription Optimized buffer (Promega) supplemented with 10 mM DTT, 0.5 L RNAsin, 2 L of rNTP nucleotide mix (5 mM ATP, 5 mM CTP, 1 mM GTP and 0.5 mM UTP), 1 L of 10 mM G-cap analog, 2.5 L of [α32P] UTP (10 mCi/L), 1 L of T7 RNA polymerase, and 2 g of DNA template.

    Electrophoretic Mobility Shift Assay:

    Article Title:
    Article Snippet: .. Electrophoretic mobility shift assay The pF3A WG vectors containing the CDS of ANAC013, ANAC016 or ANAC017 fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol, but incubating the reaction for 3 h. Expression of proteins was validated by performing a western blot against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). ..

    FLAG-tag:

    Article Title:
    Article Snippet: .. Electrophoretic mobility shift assay The pF3A WG vectors containing the CDS of ANAC013, ANAC016 or ANAC017 fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol, but incubating the reaction for 3 h. Expression of proteins was validated by performing a western blot against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). ..

    Expressing:

    Article Title:
    Article Snippet: .. Electrophoretic mobility shift assay The pF3A WG vectors containing the CDS of ANAC013, ANAC016 or ANAC017 fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol, but incubating the reaction for 3 h. Expression of proteins was validated by performing a western blot against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). ..

    Western Blot:

    Article Title:
    Article Snippet: .. Electrophoretic mobility shift assay The pF3A WG vectors containing the CDS of ANAC013, ANAC016 or ANAC017 fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol, but incubating the reaction for 3 h. Expression of proteins was validated by performing a western blot against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). ..

    other:

    Article Title:
    Article Snippet: 148 For semi-in vivo pull-down EMF2-3Flag, SEAIR and its truncations were in vitro transcribed using 149 RiboMAX Large Scale RNA Production Systems-T7 (Promega) and were labelled with biotin using 150 the Biotin RNA Labeling Mix (Roche, Indianapolis).

    Article Title: Listerin promotes cGAS protein degradation through ESCRT pathway to negatively regulate cGAS-mediated immune response
    Article Snippet: Flag-TRIM27, Flag-Listerin, Flag-Listerin (ΔHEAT) and Flag-Listerin (ΔR) proteins were expressed with a TNT Quick Coupled Transcription/Translation System kit (Promega, L1171) according to the manufacturer’s protocol. cGAS protein expressed in E. coli BL21(DE3) was a gift from Deyu Zhu (ShanDong university, China).

    Article Title: Mechanistic basis for potassium efflux driven activation of the human NLRP1 inflammasome
    Article Snippet: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations.



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