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Promega pure yield plasmid miniprep system
Pure Yield Plasmid Miniprep System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arrayjet+printing+buffer/pureyield+plasmid+miniprep+system/pmc08831226-46-138-150
Average 90 stars, based on 1 article reviews
pure yield plasmid miniprep system - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Genomic similarity of carbapenem-resistant Enterobacterales collected from mothers and their neonates.
Article Snippet: Plasmid types in transconjugants were identified by PCR-based replicon typing (PBRT) (DIATHEVA, Italy). .. The transconjugants which harboured the similar plasmid incompatibility groups with same/different blaNDM-variants within mother-neonate pairs were processed for plasmid DNA isolation using PureYieldTM plasmid Midiprep System kit (Promega Corporation, USA) according to the manufacturers’ protocol and run on 0.8% agarose gel. ..

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae
Article Snippet: .. Plasmid DNA was isolated using the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Article Title: Genistein and Vanadate Differentially Modulate Cortical GABAA Receptor/ATPase Activity and Behavior in Rats via a Phenol-Sensitive Mechanism
Article Snippet: .. Isolation and purification of plasmids were performed with the PureYieldTM Plasmid Miniprep System (Promega, Madison, WI, USA) and Plasmid Midiprep 2.0 (Evrogen, Moscow, Russia). ..

Article Title: MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control
Article Snippet: PCR products were cloned into PCR-linearized pUC19 vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, no. E2621) and transformed to DynaCompetent Cells JetGiga E. coli DH5α (BioDynamics Laboratory, no. DS230). .. After culturing E. coli at 37°C for 16 h with 3 mL LB/ampicillin (Amp) media, plasmids were purified by the PureYield Plasmid Miniprep System (Promega, no. A1223). piRFP670-N1 was gift from V. Verkhusha (Addgene plasmid #45457; http://n2t.net/addgene:45457 ; RRID:Addgene_45457). .. All plasmid sequences were checked by commercial Sanger sequencing (Eurofins Genomics or Azenta Life Sciences).

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae.
Article Snippet: .. PlasmidDNAwas isolatedusing the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Article Title: The quantitative impact of 3′UTRs on gene expression
Article Snippet: .. The next day, bacterial cultures were pooled, split into tubes, pelleted, and DNA was isolated using the Promega PureYield Plasmid Midiprep Kit. ..

DNA Extraction:

Article Title: Genomic similarity of carbapenem-resistant Enterobacterales collected from mothers and their neonates.
Article Snippet: Plasmid types in transconjugants were identified by PCR-based replicon typing (PBRT) (DIATHEVA, Italy). .. The transconjugants which harboured the similar plasmid incompatibility groups with same/different blaNDM-variants within mother-neonate pairs were processed for plasmid DNA isolation using PureYieldTM plasmid Midiprep System kit (Promega Corporation, USA) according to the manufacturers’ protocol and run on 0.8% agarose gel. ..

Agarose Gel Electrophoresis:

Article Title: Genomic similarity of carbapenem-resistant Enterobacterales collected from mothers and their neonates.
Article Snippet: Plasmid types in transconjugants were identified by PCR-based replicon typing (PBRT) (DIATHEVA, Italy). .. The transconjugants which harboured the similar plasmid incompatibility groups with same/different blaNDM-variants within mother-neonate pairs were processed for plasmid DNA isolation using PureYieldTM plasmid Midiprep System kit (Promega Corporation, USA) according to the manufacturers’ protocol and run on 0.8% agarose gel. ..

Isolation:

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae
Article Snippet: .. Plasmid DNA was isolated using the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Article Title: Genistein and Vanadate Differentially Modulate Cortical GABAA Receptor/ATPase Activity and Behavior in Rats via a Phenol-Sensitive Mechanism
Article Snippet: .. Isolation and purification of plasmids were performed with the PureYieldTM Plasmid Miniprep System (Promega, Madison, WI, USA) and Plasmid Midiprep 2.0 (Evrogen, Moscow, Russia). ..

Article Title: The quantitative impact of 3′UTRs on gene expression
Article Snippet: .. The next day, bacterial cultures were pooled, split into tubes, pelleted, and DNA was isolated using the Promega PureYield Plasmid Midiprep Kit. ..

Sequencing:

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae
Article Snippet: .. Plasmid DNA was isolated using the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae.
Article Snippet: .. PlasmidDNAwas isolatedusing the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Transformation Assay:

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae
Article Snippet: .. Plasmid DNA was isolated using the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Article Title: Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae.
Article Snippet: .. PlasmidDNAwas isolatedusing the Promega PureYield Plasmid Miniprep kit, and after confirmation by sequencing, transformed into the same S. pneumoniae background strain as the pooled library. ..

Purification:

Article Title: Genistein and Vanadate Differentially Modulate Cortical GABAA Receptor/ATPase Activity and Behavior in Rats via a Phenol-Sensitive Mechanism
Article Snippet: .. Isolation and purification of plasmids were performed with the PureYieldTM Plasmid Miniprep System (Promega, Madison, WI, USA) and Plasmid Midiprep 2.0 (Evrogen, Moscow, Russia). ..

Article Title: MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control
Article Snippet: PCR products were cloned into PCR-linearized pUC19 vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, no. E2621) and transformed to DynaCompetent Cells JetGiga E. coli DH5α (BioDynamics Laboratory, no. DS230). .. After culturing E. coli at 37°C for 16 h with 3 mL LB/ampicillin (Amp) media, plasmids were purified by the PureYield Plasmid Miniprep System (Promega, no. A1223). piRFP670-N1 was gift from V. Verkhusha (Addgene plasmid #45457; http://n2t.net/addgene:45457 ; RRID:Addgene_45457). .. All plasmid sequences were checked by commercial Sanger sequencing (Eurofins Genomics or Azenta Life Sciences).

other:

Article Title: Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression.
Article Snippet: We established a novel knockin technique, New and Easy Xenopus Targeted integration (NEXTi), to recapitulate endogenous gene expression by reporter expression.. NEXTi is a CRISPRCas9based method to integrate a donor DNA containing a reporter gene (egfp) into the target 5′ untranslated region (UTR) of the Xenopus laevis genome.. It enables us to track eGFP expression under the regulation of endogenous promoter/enhancer activities.



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