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nitrocellulose membrane  (Danaher Inc)


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    Structured Review

    Danaher Inc nitrocellulose membrane
    Nitrocellulose Membrane, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 98/100, based on 13735 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arrayjet+printing+buffer/Cellulose+Nitrate+Membrane%2C+plain%2C+sterile%2C+pore+size%2C+circle/pmc06851777-107-38-40
    Average 98 stars, based on 13735 article reviews
    nitrocellulose membrane - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Pharmacologic inhibition of NLRP3 reduces the levels of α-synuclein and protects dopaminergic neurons in a model of Parkinson’s disease
    Article Snippet: .. 30 μg of protein per sample were resolved in a Mini-PROTEAN TGX 4–20% gradient gel and transferred to a 0.1 μm nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). .. Membranes were incubated overnight with primary antibody for mouse a-synuclein (1:1000, Cell Signaling) or TREM2 (1:1000, Cell Signaling).

    Article Title: Identification of novel B cell epitopes in Fiber-2 protein of duck adenovirus 3 and their application
    Article Snippet: LMH cells infected with DAdV-3 or transfected with pcDNA3.1-Fiber-2 were washed and lysed with RIPA buffer (CWbio, Beijing, China) containing protease and phosphatase inhibitors (CST, MA, USA). .. The lysates mixed with loading buffer were boiled for 10 min and centrifuged for 3 min. After SDS-PAGE, the denatured proteins were transferred onto nitrocellulose membrane (GE Healthcare Life sciences, Freiburg, Germany). ..

    Article Title: Structure-guided design and optimization of covalent VHL-targeted sulfonyl fluoride PROTACs
    Article Snippet: The protein or lysate samples were separated on 12% (w/v) acrylamide TrisHCl gels using Tris/glycine/SDS running buffer (0.25 M Tris, 0.2 M glycine, 0.1% (w/v) SDS) at 90 V for 15 min followed by 150 V for 1 h. Protein MW markers: Precision Plus All Blue Standards or Precision Plus Protein Dual Color Standards, Bio-Rad. .. Gels were briefly washed with deionised water, and the proteins were then transferred to a 0.45 μm nitrocellulose membrane (AmershamTM Protran®, GE Healthcare) using a wet-tank transfer (Bio-Rad) in Tris-Glycine transfer buffer (25 mM Tris, 190 mM glycine and 20% v/v MeOH) for 1 h at 100 V. For the streptavidin shift and pull down assays membranes were blocked in 5% (w/v) dried skimmed milk in Tris-buffer (50 mM Tris pH 7.4, 150 mM NaCl) containing 0.1% (v/v) Tween-20 (TBS-T) for 1 h at room temperature before incubation with the appropriate primary antibody in 5% (w/v) dried skimmed milk in TBS-T overnight at 4 °C ( ). ..

    Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers
    Article Snippet: .. 24 h later, they were incubated with medium, rhIL-17 (100 ng/ml) or conditioned media of Th17 cells (diluted 1:5) for 72 h. To prepare whole cell extracts, cells were lysed with lysis buffer (130 mM Tris/HCl, 6 % SDS, 10 % 3-Mercapto-1,2-propanediol, 10 % glycerol) and 3 times treated with ultrasound for 5 s. 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). .. Rabbit polyclonal anti-SDHC (1:200; AB_2634976; Fisher Scientific), rabbit polyclonal anti-SDHD (1:200; AB_2717499; Fisher Scientific), mouse anti-e-cadherin (1:1000; AB_2728770; Cell Signaling), rabbit anti-vimentin (1:1000; AB_10695459; Cell Signaling) and mouse anti-β-Actin antibody (1:5000; AC-15; AB_476744; Sigma-Aldrich) were used.

    Article Title: Cytoplasmic aggregation of mutant FUS causes multistep RNA splicing perturbations in the course of motor neuron pathology
    Article Snippet: .. After SDS–PAGE, proteins were transferred to a nitrocellulose membrane (GE Healthcare, UK). .. We used a commercial polyclonal anti-TLS/FUS antibody (ab23439, Abcam, UK) for the detection of both transgenic and endoFUS proteins.

    Article Title: Effects of Cyanobacterial Harmful Algal Bloom Toxin Microcystin-LR on Gonadotropin-Dependent Ovarian Follicle Maturation and Ovulation in Mice
    Article Snippet: Twenty to 25 mouse follicles or ∼ 20,000 human primarily granulosa cells in each group were pooled together and lysed in ice-cold Laemmli sample buffer (Bio-Rad) containing the protease and phosphatase inhibitor cocktail (ThermoFisher Scientific), and the lysate was separated on 12% acrylamide/bisacrylamide gels. .. Proteins were transferred to a 0.45 - μ m nitrocellulose blotting membrane (GE Health care Life Science). ..

    Article Title: Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression
    Article Snippet: .. Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (GE Healtcare) for immunoblotting analysis. .. Immunoblots were performed using the following primary antibodies: anti-Rai (BD, #610643), anti-pGSK3α/β (Cell Signaling, #8566), anti-pAkt (Cell Signaling, #9271), anti-actin (clone C4, Millipore, #MAB1501) and peroxidase-labeled secondary antibodies (anti-rabbit cod: 111-035-144 and anti-mouse cod:115-035-146 Jackson ImmunoResearch Laboratories).

    Article Title: Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation
    Article Snippet: .. Samples were separated in a 10% acrylamide (Roth, Karlsruhe, Germany) gel and blotted onto nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in a wet system. .. Protein content was measured with Bradford assay (Bio-Rad).

    Incubation:

    Article Title: Structure-guided design and optimization of covalent VHL-targeted sulfonyl fluoride PROTACs
    Article Snippet: The protein or lysate samples were separated on 12% (w/v) acrylamide TrisHCl gels using Tris/glycine/SDS running buffer (0.25 M Tris, 0.2 M glycine, 0.1% (w/v) SDS) at 90 V for 15 min followed by 150 V for 1 h. Protein MW markers: Precision Plus All Blue Standards or Precision Plus Protein Dual Color Standards, Bio-Rad. .. Gels were briefly washed with deionised water, and the proteins were then transferred to a 0.45 μm nitrocellulose membrane (AmershamTM Protran®, GE Healthcare) using a wet-tank transfer (Bio-Rad) in Tris-Glycine transfer buffer (25 mM Tris, 190 mM glycine and 20% v/v MeOH) for 1 h at 100 V. For the streptavidin shift and pull down assays membranes were blocked in 5% (w/v) dried skimmed milk in Tris-buffer (50 mM Tris pH 7.4, 150 mM NaCl) containing 0.1% (v/v) Tween-20 (TBS-T) for 1 h at room temperature before incubation with the appropriate primary antibody in 5% (w/v) dried skimmed milk in TBS-T overnight at 4 °C ( ). ..

    Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers
    Article Snippet: .. 24 h later, they were incubated with medium, rhIL-17 (100 ng/ml) or conditioned media of Th17 cells (diluted 1:5) for 72 h. To prepare whole cell extracts, cells were lysed with lysis buffer (130 mM Tris/HCl, 6 % SDS, 10 % 3-Mercapto-1,2-propanediol, 10 % glycerol) and 3 times treated with ultrasound for 5 s. 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). .. Rabbit polyclonal anti-SDHC (1:200; AB_2634976; Fisher Scientific), rabbit polyclonal anti-SDHD (1:200; AB_2717499; Fisher Scientific), mouse anti-e-cadherin (1:1000; AB_2728770; Cell Signaling), rabbit anti-vimentin (1:1000; AB_10695459; Cell Signaling) and mouse anti-β-Actin antibody (1:5000; AC-15; AB_476744; Sigma-Aldrich) were used.

    Lysis:

    Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers
    Article Snippet: .. 24 h later, they were incubated with medium, rhIL-17 (100 ng/ml) or conditioned media of Th17 cells (diluted 1:5) for 72 h. To prepare whole cell extracts, cells were lysed with lysis buffer (130 mM Tris/HCl, 6 % SDS, 10 % 3-Mercapto-1,2-propanediol, 10 % glycerol) and 3 times treated with ultrasound for 5 s. 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). .. Rabbit polyclonal anti-SDHC (1:200; AB_2634976; Fisher Scientific), rabbit polyclonal anti-SDHD (1:200; AB_2717499; Fisher Scientific), mouse anti-e-cadherin (1:1000; AB_2728770; Cell Signaling), rabbit anti-vimentin (1:1000; AB_10695459; Cell Signaling) and mouse anti-β-Actin antibody (1:5000; AC-15; AB_476744; Sigma-Aldrich) were used.

    SDS-Gel:

    Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers
    Article Snippet: .. 24 h later, they were incubated with medium, rhIL-17 (100 ng/ml) or conditioned media of Th17 cells (diluted 1:5) for 72 h. To prepare whole cell extracts, cells were lysed with lysis buffer (130 mM Tris/HCl, 6 % SDS, 10 % 3-Mercapto-1,2-propanediol, 10 % glycerol) and 3 times treated with ultrasound for 5 s. 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). .. Rabbit polyclonal anti-SDHC (1:200; AB_2634976; Fisher Scientific), rabbit polyclonal anti-SDHD (1:200; AB_2717499; Fisher Scientific), mouse anti-e-cadherin (1:1000; AB_2728770; Cell Signaling), rabbit anti-vimentin (1:1000; AB_10695459; Cell Signaling) and mouse anti-β-Actin antibody (1:5000; AC-15; AB_476744; Sigma-Aldrich) were used.

    Electrophoresis:

    Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers
    Article Snippet: .. 24 h later, they were incubated with medium, rhIL-17 (100 ng/ml) or conditioned media of Th17 cells (diluted 1:5) for 72 h. To prepare whole cell extracts, cells were lysed with lysis buffer (130 mM Tris/HCl, 6 % SDS, 10 % 3-Mercapto-1,2-propanediol, 10 % glycerol) and 3 times treated with ultrasound for 5 s. 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). .. Rabbit polyclonal anti-SDHC (1:200; AB_2634976; Fisher Scientific), rabbit polyclonal anti-SDHD (1:200; AB_2717499; Fisher Scientific), mouse anti-e-cadherin (1:1000; AB_2728770; Cell Signaling), rabbit anti-vimentin (1:1000; AB_10695459; Cell Signaling) and mouse anti-β-Actin antibody (1:5000; AC-15; AB_476744; Sigma-Aldrich) were used.

    SDS Page:

    Article Title: Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression
    Article Snippet: .. Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (GE Healtcare) for immunoblotting analysis. .. Immunoblots were performed using the following primary antibodies: anti-Rai (BD, #610643), anti-pGSK3α/β (Cell Signaling, #8566), anti-pAkt (Cell Signaling, #9271), anti-actin (clone C4, Millipore, #MAB1501) and peroxidase-labeled secondary antibodies (anti-rabbit cod: 111-035-144 and anti-mouse cod:115-035-146 Jackson ImmunoResearch Laboratories).

    Western Blot:

    Article Title: Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression
    Article Snippet: .. Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (GE Healtcare) for immunoblotting analysis. .. Immunoblots were performed using the following primary antibodies: anti-Rai (BD, #610643), anti-pGSK3α/β (Cell Signaling, #8566), anti-pAkt (Cell Signaling, #9271), anti-actin (clone C4, Millipore, #MAB1501) and peroxidase-labeled secondary antibodies (anti-rabbit cod: 111-035-144 and anti-mouse cod:115-035-146 Jackson ImmunoResearch Laboratories).



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