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glass microscope slides schott nexterion d  (SCHOTT)

 
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    Structured Review

    SCHOTT glass microscope slides schott nexterion d
    (top) Resistance of gold- and ITO-coated <t>microscope</t> slides before and after DNA synthesis under iodine or CSO-mediated oxidation conditions. Measurements were taken with an ohmmeter at three diagonally opposed locations (bottom): slide corners (green), within the reaction chamber (red), and within the MAS synthesis area (blue). Sheet resistance (in Ω/square) was estimated by dividing the resistance with the length/width ratio of the rectangle encasing the two measurement points. The ratio was ∼3:1 for the slide dimensions, 2:1 for the reaction chamber, and 1.4:1 for the synthesis area. The sheet resistance and electrical resistance should be understood as approximate values only with an error margin of at least ±10% across three independent measurements.
    Glass Microscope Slides Schott Nexterion D, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+superepoxy+microscope+slides/glass+slides+schott+nexterion/pmc10867803-35-6-9
    Average 90 stars, based on 1 article reviews
    glass microscope slides schott nexterion d - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates"

    Article Title: Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates

    Journal: Analytical Chemistry

    doi: 10.1021/acs.analchem.3c04166

    (top) Resistance of gold- and ITO-coated microscope slides before and after DNA synthesis under iodine or CSO-mediated oxidation conditions. Measurements were taken with an ohmmeter at three diagonally opposed locations (bottom): slide corners (green), within the reaction chamber (red), and within the MAS synthesis area (blue). Sheet resistance (in Ω/square) was estimated by dividing the resistance with the length/width ratio of the rectangle encasing the two measurement points. The ratio was ∼3:1 for the slide dimensions, 2:1 for the reaction chamber, and 1.4:1 for the synthesis area. The sheet resistance and electrical resistance should be understood as approximate values only with an error margin of at least ±10% across three independent measurements.
    Figure Legend Snippet: (top) Resistance of gold- and ITO-coated microscope slides before and after DNA synthesis under iodine or CSO-mediated oxidation conditions. Measurements were taken with an ohmmeter at three diagonally opposed locations (bottom): slide corners (green), within the reaction chamber (red), and within the MAS synthesis area (blue). Sheet resistance (in Ω/square) was estimated by dividing the resistance with the length/width ratio of the rectangle encasing the two measurement points. The ratio was ∼3:1 for the slide dimensions, 2:1 for the reaction chamber, and 1.4:1 for the synthesis area. The sheet resistance and electrical resistance should be understood as approximate values only with an error margin of at least ±10% across three independent measurements.

    Techniques Used: Microscopy, DNA Synthesis

    (A) Fluorescence signals with a Cy3-labeled complementary sequence hybridized onto 25mer DNA microarrays decorated with ∼2000 replicates of the same sequence. Synthesis was performed using either iodine/water (yellow bars) or CSO (blue bars) as the oxidizer and otherwise standard photolithographic conditions on gold- or ITO-coated microscope slides. The glass copy (gold/ITO) labeling refers to the signal from the glass slide when synthesized alongside a gold- or ITO-coated substrate. (B) The background signal is recorded from features that only contain a dT 5 linker. (C) The signal/background corresponds to the ratio between hybridization signal and background fluorescence. (D) The dual array ratio is the ratio between fluorescence signals on the conductive substrate and those of the glass microscope slide. Error bars are the SD. (E) In the dual-array MAS procedure, a second microarray is produced simultaneously on a common silanized glass slide (“glass copy”). (F) Excerpts of microarray scans after hybridization with a Cy3-labeled complementary 25mer DNA strand. Hybridization was performed on gold and ITO-coated microscope slides and their duplicate on silanized glass. Each feature is a matrix of 5 × 5 micromirrors with a one-mirror wide gap between adjacent features. Features are randomly distributed across the entire microarray, and all contain the same 25mer DNA sequence. Excerpts are ∼0.5% of the total synthesis area. The scale bar is ∼100 μm.
    Figure Legend Snippet: (A) Fluorescence signals with a Cy3-labeled complementary sequence hybridized onto 25mer DNA microarrays decorated with ∼2000 replicates of the same sequence. Synthesis was performed using either iodine/water (yellow bars) or CSO (blue bars) as the oxidizer and otherwise standard photolithographic conditions on gold- or ITO-coated microscope slides. The glass copy (gold/ITO) labeling refers to the signal from the glass slide when synthesized alongside a gold- or ITO-coated substrate. (B) The background signal is recorded from features that only contain a dT 5 linker. (C) The signal/background corresponds to the ratio between hybridization signal and background fluorescence. (D) The dual array ratio is the ratio between fluorescence signals on the conductive substrate and those of the glass microscope slide. Error bars are the SD. (E) In the dual-array MAS procedure, a second microarray is produced simultaneously on a common silanized glass slide (“glass copy”). (F) Excerpts of microarray scans after hybridization with a Cy3-labeled complementary 25mer DNA strand. Hybridization was performed on gold and ITO-coated microscope slides and their duplicate on silanized glass. Each feature is a matrix of 5 × 5 micromirrors with a one-mirror wide gap between adjacent features. Features are randomly distributed across the entire microarray, and all contain the same 25mer DNA sequence. Excerpts are ∼0.5% of the total synthesis area. The scale bar is ∼100 μm.

    Techniques Used: Fluorescence, Labeling, Sequencing, Microscopy, Synthesized, Hybridization, Microarray, Produced

    Related Articles

    In Situ:

    Article Title: Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase.
    Article Snippet: .. The detailed procedure for photolithographic in situ synthesis has already been described elsewhere.47,48 Briefly, microscopy glass slides (Schott NEXTERION glass D) were functionalized with a 2% N-(3-triethoxysilylpropyl)-4-hydroxybutyramide (95%; abcr) solution in ethanol/water/acetic acid (95:5:0.1), washed, and cured at 120 °C under a vacuum for 2 h. An Expedite 8909 nucleic acid synthesizer was used to deliver reagents for synthesis to the glass substrate. .. Anhydrous acetonitrile (Biosolve) and DCI activator (Sigma-Aldrich, L032000) were maintained dry under molecular sieves (Sigma-Aldrich, Z509027).

    Microscopy:

    Article Title: Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase.
    Article Snippet: .. The detailed procedure for photolithographic in situ synthesis has already been described elsewhere.47,48 Briefly, microscopy glass slides (Schott NEXTERION glass D) were functionalized with a 2% N-(3-triethoxysilylpropyl)-4-hydroxybutyramide (95%; abcr) solution in ethanol/water/acetic acid (95:5:0.1), washed, and cured at 120 °C under a vacuum for 2 h. An Expedite 8909 nucleic acid synthesizer was used to deliver reagents for synthesis to the glass substrate. .. Anhydrous acetonitrile (Biosolve) and DCI activator (Sigma-Aldrich, L032000) were maintained dry under molecular sieves (Sigma-Aldrich, Z509027).

    Article Title: Miniaturized Drug Sensitivity and Resistance Test on Patient-Derived Cells Using Droplet-Microarray.
    Article Snippet: .. Microscope glass slides (Schott Nexterion, Jena, Germany) were fluorinated by trichloro(1H,1H,2H,2Hperfluorooctyl)silane (Sigma-Aldrich, Chemie) under a ..

    Article Title: An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting
    Article Snippet: .. The synthesis occurs on glass microscope slides (Schott Nexterion D) which have been silanized with hydroxybutyramide prior to synthesis. ..

    other:

    Article Title: Palladium-Catalyzed Combinatorial Synthesis of Biphenyls on Droplet Microarrays at Nanoliter Scale.
    Article Snippet: Glass slides were purchased from Schott Nexterion (Jena, Germany).

    Article Title: Spatial reorganization of analytes in charged aqueous microdroplets
    Article Snippet: Clean glass slides (SCHOTT Nexterion®) were used for all the imaging experiments.

    Clinical Proteomics:

    Article Title: Interplay of structure and photophysics of individualized rod-shaped graphene quantum dots with up to 132 sp ² carbon atoms
    Article Snippet: .. The mixture is spin-coated on glass substrate (Schott Nexterion) previously plasma-cleaned for 5 min. ..

    Microarray:

    Article Title: Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates
    Article Snippet: Oxidizers were either 0.02 M I 2 in tetrahydrofuran/pyridine/H 2 O 90:9:0.4 (Sigma L060020) or 0.5 M CSO in ACN (Eurogentec 40–4632–52E). .. Microarray synthesis was carried out on glass microscope slides (Schott Nexterion D) silane-functionalized with N -(3-triethoxysilylpropyl)-4-hydroxybutyramide (Gelest SIT8189.5), gold-coated microscope slides with a 3D-amine layer (PolyAn 109 10008), or ITO-coated SuperEpoxy 2 microscope slides (ArrayIt). .. Deprotection was performed with a 1:1 mixture of ethylenediamine (EDA, Sigma 03550) in ethanol (VWR 1009831000), and the cleavage and deprotection of oligonucleotide libraries were alternatively carried out in a 1:1 solution of EDA in toluene (Sigma 244511).



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