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Characterization of the autologous hair follicle secretome (aHFS). ( A <t>)</t> <t>Semi-quantitative</t> analysis of selected protein analytes in aHFS using a <t>Quantibody</t> ® <t>glass</t> <t>slide-based</t> array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.
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Characterization of the autologous hair follicle secretome (aHFS). ( A <t>)</t> <t>Semi-quantitative</t> analysis of selected protein analytes in aHFS using a <t>Quantibody</t> ® <t>glass</t> <t>slide-based</t> array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.
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Characterization of the autologous hair follicle secretome (aHFS). ( A ) Semi-quantitative analysis of selected protein analytes in aHFS using a Quantibody ® glass slide-based array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.

Journal: International Journal of Molecular Sciences

Article Title: The Autologous Hair Follicle and Its Secretome: A Multipotent Source for Cell-Based and Cell-Free Regenerative Therapies

doi: 10.3390/ijms27104183

Figure Lengend Snippet: Characterization of the autologous hair follicle secretome (aHFS). ( A ) Semi-quantitative analysis of selected protein analytes in aHFS using a Quantibody ® glass slide-based array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.

Article Snippet: A semi-quantitative Quantibody ® glass slide-based cytokine array (RayBiotech, Peachtree Corners, GA, USA; Cat. #QAM-CAA-4000) was utilized to profile growth factors and cytokines.

Techniques: Concentration Assay, Labeling, Scratch Wound Assay Assay, Control