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rt2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit  (Qiagen)


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    Qiagen rt2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit
    Rt2 Profiler Pcr Array—Human Dna Damage Signaling Pathway Gene Expression Evaluation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+gene+expression+profiling/human+dna+damage+signaling+pathway+rt2+profiler+pcr+array/pm37886943-236-18-30
    Average 90 stars, based on 1 article reviews
    rt2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions.
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    Article Title: Cytotoxic, genotoxic, and toxicogenomic effects of heated tobacco products and cigarette smoke in human primary keratinocytes
    Article Snippet: RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Reverse transcription was performed using the miRCURY LNA RT Kit (Qiagen, Germany), and mRNA expression profiling of RNA samples was performed using a Human DNA Damage Signaling RT2 Profiler PCR Array (Qiagen, Germany). ..

    Real-time Polymerase Chain Reaction:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions.
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    Polymerase Chain Reaction:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions.
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    Article Title: Cytotoxic, genotoxic, and toxicogenomic effects of heated tobacco products and cigarette smoke in human primary keratinocytes
    Article Snippet: RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Reverse transcription was performed using the miRCURY LNA RT Kit (Qiagen, Germany), and mRNA expression profiling of RNA samples was performed using a Human DNA Damage Signaling RT2 Profiler PCR Array (Qiagen, Germany). ..

    Article Title: A Novel DNA Repair-Gene Model to Predict Responses to Immunotherapy and Prognosis in Patients With EGFR-Mutant Non-Small Cell Lung Cancer.
    Article Snippet: .. A total of 84 DDR genes were obtained from GeneGlobe Design & Analysis Hub: RT2 Profiler PCR Array Human DNA Damage Signaling Pathway (https:// geneg lobe. qiagen. com/ ro/ produ ct- groups/ rt2- profi ler- pcr- arrays/ PAHS- 029Z). ..

    Article Title: Aldehyde Dehydrogenase 1B1 Is Implicated in DNA Damage Response in Human Colorectal Adenocarcinoma
    Article Snippet: Goat anti-rabbit and mouse IgG horseradish peroxidase conjugated antibodies were from Millipore (Burlington, MA, USA). .. The RT 2 Profiler PCR array for the DNA damage signaling pathway was purchased from Qiagen (Venlo, The Netherlands). ..

    Gene Expression:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions.
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    other:

    Article Title: Silencing of the DNA damage repair regulator PPP1R15A sensitizes acute myeloid leukemia cells to chemotherapy.
    Article Snippet: Gene expression profiling through PCR arrays analysis (Qiagen RT2 Profiler PCR Array (96-Well Format and 384-Well [4 × 96] Format) Human DNA Damage Signaling Pathway) was performed in triplicate after RNA extraction from untreated, chemotherapy-treated, and live cells following chemotherapy exposure.

    Article Title: RAD51B Activity and Cell Cycle Regulation in Response to DNA Damage in Breast Cancer Cell Lines
    Article Snippet: Background was subtracted, and quantile-normalized data were analyzed using GenomeStudio (Illumina) and Gene Set Enrichment Analysis (GSEA; Broad Institute) software packages.

    Article Title: Evaluation of weak genotoxicity of hydroxychloroquine in human TK6 cells.
    Article Snippet: Hydroxychloroquine (HCQ), a derivative of chloroquine (CQ), is an antimalarial and antirheumatic drug.. Since there is limited data available on the genotoxicity of HCQ, in the current study, we used a battery of in vitro assays to systematically examine the genotoxicity of HCQ in human lymphoblastoid TK6 cells.. We first showed that HCQ is not mutagenic in TK6 cells up to 80 μM with or without exogenous metabolic activation.

    Article Title: Regenerative Capacity of Old Muscle Stem Cells Declines without Significant Accumulation of DNA Damage
    Article Snippet: HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnologies.

    Reverse Transcription:

    Article Title: Cytotoxic, genotoxic, and toxicogenomic effects of heated tobacco products and cigarette smoke in human primary keratinocytes
    Article Snippet: RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Reverse transcription was performed using the miRCURY LNA RT Kit (Qiagen, Germany), and mRNA expression profiling of RNA samples was performed using a Human DNA Damage Signaling RT2 Profiler PCR Array (Qiagen, Germany). ..



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    Image Search Results


    Genes analyzed with the  RT 2 Profiler PCR Array—Human  DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Journal: Current Issues in Molecular Biology

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions

    doi: 10.3390/cimb45100500

    Figure Lengend Snippet: Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Article Snippet: Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA).

    Techniques:

    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk

    doi: 10.3389/fcimb.2022.1035711

    Figure Lengend Snippet: Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Article Snippet: After that, PBMC were harvested for immune genes profiling with customized RT2 Profiler PCR Array Gene Expression Kit (Qiagen).

    Techniques: Infection, Gene Expression, Control, Expressing, Staining, Flow Cytometry

    Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Journal: BMC Genomics

    Article Title: A comprehensive platform for highly multiplexed mammalian functional genetic screens

    doi: 10.1186/1471-2164-12-213

    Figure Lengend Snippet: Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Article Snippet: For comparative purposes, we also included up to three additional features found on the human expression profiling Affymetrix Gene 1.0 ST array for 18,088 of these sequences.

    Techniques: Amplification, Hybridization