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integrated web-based software package for the pcr array system  (SuperArray Bioscience Corporation)

 
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    Structured Review

    SuperArray Bioscience Corporation integrated web-based software package for the pcr array system
    CK2 inhibitor affects p53 target genes associated with cell cycle regulation <t>and</t> <t>apoptosis.</t> ( a ) CK2-I increases p21 expression in a p53-dependent manner. Representative blot is shown from three independent experiments with identical results. Blots were re-probed with c23 to establish equivalent loading. ( b ) CK2-I increases mRNA levels of pro-apoptotic molecules Noxa and GADD45 β in a p53-dependent manner. Total RNA was isolated from cells treated with different combinations of TNF α and CK2-I, and the mRNA levels for Noxa, GADD45 β and constitutive enzyme GAPDH were determined by <t>RT-PCR.</t> ( c ) Pifithrin- α reverses CK2-I-mediated G2/M phase arrest in A172 cells. FACS analysis was performed on A172 cells treated with different combinations of TNF α , CK2-Is and Pifithrin- α . Inset indicates percentage of cells in G1, S and G2/M phase of the cell cycle. C, T, Pf and A denote control, TNF α , Pifithrin- α and Apigenin, respectively
    Integrated Web Based Software Package For The Pcr Array System, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+design+software+package/integrated+web+based+software+package+for+the+pcr+array+system/pmc03288342-223-15-18
    Average 90 stars, based on 1 article reviews
    integrated web-based software package for the pcr array system - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Inhibition of Casein kinase-2 induces p53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor (TNF α )-induced apoptosis through SIRT1 inhibition"

    Article Title: Inhibition of Casein kinase-2 induces p53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor (TNF α )-induced apoptosis through SIRT1 inhibition

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2012.10

    CK2 inhibitor affects p53 target genes associated with cell cycle regulation and apoptosis. ( a ) CK2-I increases p21 expression in a p53-dependent manner. Representative blot is shown from three independent experiments with identical results. Blots were re-probed with c23 to establish equivalent loading. ( b ) CK2-I increases mRNA levels of pro-apoptotic molecules Noxa and GADD45 β in a p53-dependent manner. Total RNA was isolated from cells treated with different combinations of TNF α and CK2-I, and the mRNA levels for Noxa, GADD45 β and constitutive enzyme GAPDH were determined by RT-PCR. ( c ) Pifithrin- α reverses CK2-I-mediated G2/M phase arrest in A172 cells. FACS analysis was performed on A172 cells treated with different combinations of TNF α , CK2-Is and Pifithrin- α . Inset indicates percentage of cells in G1, S and G2/M phase of the cell cycle. C, T, Pf and A denote control, TNF α , Pifithrin- α and Apigenin, respectively
    Figure Legend Snippet: CK2 inhibitor affects p53 target genes associated with cell cycle regulation and apoptosis. ( a ) CK2-I increases p21 expression in a p53-dependent manner. Representative blot is shown from three independent experiments with identical results. Blots were re-probed with c23 to establish equivalent loading. ( b ) CK2-I increases mRNA levels of pro-apoptotic molecules Noxa and GADD45 β in a p53-dependent manner. Total RNA was isolated from cells treated with different combinations of TNF α and CK2-I, and the mRNA levels for Noxa, GADD45 β and constitutive enzyme GAPDH were determined by RT-PCR. ( c ) Pifithrin- α reverses CK2-I-mediated G2/M phase arrest in A172 cells. FACS analysis was performed on A172 cells treated with different combinations of TNF α , CK2-Is and Pifithrin- α . Inset indicates percentage of cells in G1, S and G2/M phase of the cell cycle. C, T, Pf and A denote control, TNF α , Pifithrin- α and Apigenin, respectively

    Techniques Used: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Control

    Related Articles

    Software:

    Article Title: Inhibition of Casein kinase-2 induces p53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor (TNF α )-induced apoptosis through SIRT1 inhibition
    Article Snippet: .. Results were analyzed as per user manual guidelines using integrated web-based software package for the PCR Array System (SuperArray Biosciences RT2 Profiler PCR Array Human Apoptosis PAHS-012–A-12). ..

    Article Title: Sutherlandia frutescens prevents changes in diabetes-related gene expression in a fructose-induced insulin resistant cell model.
    Article Snippet: Ethnopharmacological relevance: The African medicinal plant Sutherlandia frutescens (L.) R.Br. (Fabaceae) is traditionally used to treat diabetes and has been shown to have anti-diabetic properties in animal models.. The present study investigated the capacity of an aqueous extract of Sutherlandia frutescens to prevent insulin resistance (a precursor of type 2 diabetes) in a human liver cell culture and to identify genes regulated by Sutherlandia frutescens treatment.. Materials and methods: A combination of insulin and fructose was used to generate an in vitro model of insulin resistance in human liver cells to compare untreated control, insulin resistant and Sutherlandia frutescens treated insulin resistant cultures.

    Polymerase Chain Reaction:

    Article Title: Inhibition of Casein kinase-2 induces p53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor (TNF α )-induced apoptosis through SIRT1 inhibition
    Article Snippet: .. Results were analyzed as per user manual guidelines using integrated web-based software package for the PCR Array System (SuperArray Biosciences RT2 Profiler PCR Array Human Apoptosis PAHS-012–A-12). ..

    Article Title: Sutherlandia frutescens prevents changes in diabetes-related gene expression in a fructose-induced insulin resistant cell model.
    Article Snippet: Ethnopharmacological relevance: The African medicinal plant Sutherlandia frutescens (L.) R.Br. (Fabaceae) is traditionally used to treat diabetes and has been shown to have anti-diabetic properties in animal models.. The present study investigated the capacity of an aqueous extract of Sutherlandia frutescens to prevent insulin resistance (a precursor of type 2 diabetes) in a human liver cell culture and to identify genes regulated by Sutherlandia frutescens treatment.. Materials and methods: A combination of insulin and fructose was used to generate an in vitro model of insulin resistance in human liver cells to compare untreated control, insulin resistant and Sutherlandia frutescens treated insulin resistant cultures.



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