Journal: IJID Regions
Article Title: Targeted deep sequencing of mycobacteria species from extrapulmonary sites not identified by routine line probe assays: A retrospective laboratory analysis of stored clinical cultures
doi: 10.1016/j.ijregi.2024.100464
Figure Lengend Snippet: A phylogenetic tree was generated using consensus rpoB gene sequences of unidentified mycobacteria species obtained through Oxford Nanopore Technology amplicon-based deep sequencing. The tree illustrates slow-growing mycobacteria represented by red branches and rapid growers indicated by blue branches. Unidentified Mycobacterium species sequences from the study are labeled in red. Members of the Mycobacterium tuberculosis complex are highlighted in the yellow range. The scale bar expresses the average number of nucleotide substitutions per site. Circular markers signify the bootstrap support of branches within the tree. The isolates included in the construction of the phylogenetic tree are those represented by the reads listed in : 1R.1, 2R.1, 6R.1, 8R.1, 9R.1, 10R.1, 10R.2, 10R.3, 10R.4, 10R.5, 15R.1, 16R.1, 18R.1, 20R.1, 21R.1, 22R.1, 23R.1, 24R.1, 26R.1, 27R.1, 28R.1.
Article Snippet: The consensus sequences were subjected to analysis through the National Centre for Biotechnology Information nucleotide Basic Local Alignment Search Tool software program [ ].
Techniques: Generated, Amplification, Sequencing, Labeling