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affinityscript ™ multiple temperature reverse transcriptase kit  (Agilent technologies)


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    Structured Review

    Agilent technologies affinityscript ™ multiple temperature reverse transcriptase kit
    Affinityscript ™ Multiple Temperature Reverse Transcriptase Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affinityscript+multiple+temperature+reverse+transcriptase+kit/pmc07815118-70-7-14
    Average 90 stars, based on 1 article reviews
    affinityscript ™ multiple temperature reverse transcriptase kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Suppression of NS3 and MP Is Important for the Stable Inheritance of RNAi-Mediated Rice Stripe Virus (RSV) Resistance Obtained by Targeting the Fully Complementary RSV-CP Gene
    Article Snippet: The first strands of cDNA were synthesized using the AffinityScriptTM Multiple Temperature Reverse Transcriptase Kit (Stratagene, USA). cDNA was amplified using 1.5 units of Ex Taq DNA polymerase (Takara, Japan), 100 μM of each dNTP, and 10 pmol of each gene-specific primer in a T1 thermal cycler (Biometra, Germany).

    Article Title: Premature Vertebral Mineralization in hmx1 -Mutant Zebrafish
    Article Snippet: Primers specific to the selected genes were designed , and first-strand cDNA synthesis using the AffinityScriptTM Multiple Temperature Reverse Transcriptase Kit (Agilent; Basel, Switzerland) was performed according to the manufacturer’s protocol. cDNA was generated (GoScript Reverse Transcriptase System; Promega; Dübendorf, Switzerland), and real-time RT-PCR (FastStart SYBR Green Master Roche; Rotkreuz, Switzerland) was performed following standard protocols.

    Article Title: Rapamycin Exerts Antifungal Activity In Vitro and In Vivo against Mucor circinelloides via FKBP12-Dependent Inhibition of Tor
    Article Snippet: Hybridized probe signals were detected using a phosphorimager. cDNA was synthesized from 1.5 μg of total RNA by use of an AffinityScript multiple-temperature reverse transcriptase kit (Stratagene).

    Article Title: Rapamycin Exerts Antifungal Activity In Vitro and In Vivo against Mucor circinelloides via FKBP12-Dependent Inhibition of Tor
    Article Snippet: Hybridized probe signals were detected using a phosphorimager. cDNA was synthesized from 1.5 g of total RNA by use of an AffinityScript multiple-temperature reverse transcriptase kit (Stratagene).

    Article Title: Successful establishment and global dispersal of genotype VI avian paramyxovirus serotype 1 after cross species transmission
    Article Snippet: Viral RNA was extracted using the QIAamp Viral RNA extraction kit (Qiagen, Valencia, CA-USA) and was reverse transcribed using a specific PMV1 oligonucleotide targeting the 3′ untranslated region of the genome (5′-ACGGGTAGAAGGTGTGAATC-3′) with the AffinityScriptTM Multiple Temperature Reverse Transcriptase Kit (Stratagene, Santa Clara, CA-USA).

    Article Title: Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish
    Article Snippet: First-strand cDNA synthesis was performed using the AffinityScript TM Multiple Temperature Reverse Transcriptase kit (Agilent, Basel, Switzerland) according the manufacturer’s protocol. cDNA was generated (GoScript Reverse Transcriptase System; Promega) and Real time PCR (FastStart SYBR Green Master Roche) was performed using uhrf1 -F 5’-TCCAGGAGTCCAAGAGAGGAA-3’ and uhrf1 -R 5’-TCTGCTGAACACAGTTCGGG-3’ ; dnmt1 -F 5’-TTACTTTGGGCAAGAGGAGAGC-3’ , dnmt1 -R 5’-GACACCACACCGTTGTCTCT-3’ ; hmx1 -F 5’-CGAAACCTCCAGGAG TCCAAG-3’ , hmx1 -R 5’-CGGGTCTTTTTCTTTCGGGC-3’ .

    Reverse Transcription:

    Article Title: Insulin Signaling Via Progesterone-Regulated Insulin Receptor Substrate 2 is Critical for Human Uterine Decidualization
    Article Snippet: Total RNA was extracted from cultured HESC using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Total RNA was converted to cDNA using the AffinityScript Multiple Temperature Reverse Transcriptase kit (Agilent), following the manufacturer’s instructions. cDNA was subjected to quantitative PCR analysis using Power Sybr Green PCR master mix (Applied Biosystems) and gene-specific primers (IDT Integrated DNA Technologies). ..

    Article Title: C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.
    Article Snippet: Hexanucleotide repeat expansions in the C9ORF72 gene are causally associatedwith frontotemporal lobar dementia (FTLD) and/or amyotrophic lateral sclerosis (ALS).. The physiological function of the normal C9ORF72 protein remains unclear.. In this study, we characterized the subcellular localization of C9ORF72 to processing bodies (P-bodies) and its recruitment to stress granules (SGs) upon stress-related stimuli.

    Real-time Polymerase Chain Reaction:

    Article Title: Insulin Signaling Via Progesterone-Regulated Insulin Receptor Substrate 2 is Critical for Human Uterine Decidualization
    Article Snippet: Total RNA was extracted from cultured HESC using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Total RNA was converted to cDNA using the AffinityScript Multiple Temperature Reverse Transcriptase kit (Agilent), following the manufacturer’s instructions. cDNA was subjected to quantitative PCR analysis using Power Sybr Green PCR master mix (Applied Biosystems) and gene-specific primers (IDT Integrated DNA Technologies). ..

    Article Title: C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.
    Article Snippet: Hexanucleotide repeat expansions in the C9ORF72 gene are causally associatedwith frontotemporal lobar dementia (FTLD) and/or amyotrophic lateral sclerosis (ALS).. The physiological function of the normal C9ORF72 protein remains unclear.. In this study, we characterized the subcellular localization of C9ORF72 to processing bodies (P-bodies) and its recruitment to stress granules (SGs) upon stress-related stimuli.

    SYBR Green Assay:

    Article Title: Insulin Signaling Via Progesterone-Regulated Insulin Receptor Substrate 2 is Critical for Human Uterine Decidualization
    Article Snippet: Total RNA was extracted from cultured HESC using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Total RNA was converted to cDNA using the AffinityScript Multiple Temperature Reverse Transcriptase kit (Agilent), following the manufacturer’s instructions. cDNA was subjected to quantitative PCR analysis using Power Sybr Green PCR master mix (Applied Biosystems) and gene-specific primers (IDT Integrated DNA Technologies). ..

    Polymerase Chain Reaction:

    Article Title: Insulin Signaling Via Progesterone-Regulated Insulin Receptor Substrate 2 is Critical for Human Uterine Decidualization
    Article Snippet: Total RNA was extracted from cultured HESC using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Total RNA was converted to cDNA using the AffinityScript Multiple Temperature Reverse Transcriptase kit (Agilent), following the manufacturer’s instructions. cDNA was subjected to quantitative PCR analysis using Power Sybr Green PCR master mix (Applied Biosystems) and gene-specific primers (IDT Integrated DNA Technologies). ..



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