hbss buffer (Thermo Fisher)

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Thermo Fisher hbss buffer
Hbss Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ae+buffer/HEPES-buffered+saline+(5X%2C+pH+7%2E4)%2C+low+endotoxin/pmc08228075-282-9-11
Average 94 stars, based on 1 article reviews

hbss buffer - by Bioz Stars, 2026-11

94/100 stars

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Transfection:

Article Title: A Prefusion Form of Herpes Simplex Virus 1 gB has a Distinct Antigenic Signature
Article Snippet: Substrate 2,2′-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS; Thermo Fisher Scientific) was added, and absorbance was measured at 405 nm using a BioTek microplate reader. .. CHO-K1 cells transfected with either pAM24 (wild type gB) or pAM28 (gB H516P) were rinsed with PBS, then lysed in either 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma Aldrich, St. Louis, MO, USA) detergent in HEPES-buffered saline (HBS) (ACROS Organics, New Jersey, USA) with Halt protease inhibitor (Rockford, IL, USA). ..

Saline:

Article Title: Glucose-driven intra- and inter-islet beta cell synchronization in pancreatic tissue slices
Article Snippet: .. For calcium dye loading, the slices were incubated in 3.33 ml of HEPES-buffered saline containing (in mM) 6 glucose, 150 NaCl, 10 HEPES, 5 KCl, 2 CaCl 2 , and 1 MgCl 2 , pH adjusted to 7.4 with 1 M NaOH, and supplemented with 3.75 μl of dimethylsulfoxide, 1.25 μl of Pluronic F-127, and either 25 μg of Oregon Green 488 BAPTA-1 (OGB-1; Invitrogen, Waltham, MA, USA) or 25 μg of Calbryte 520 AM (AAT Bioquest, Pleasanton, CA, USA). ..

Protease Inhibitor:

Incubation:

Dissection:

Article Title: Multimodal control of dendritic cell functions by nociceptors.
Article Snippet: .. Dissection was performed in ice-coldHBSS+HEPES (both Gibco), and tissues were digested at 37°C for 15min in 0.25% trypsin (Invitrogen) for hippocampus and 0.25% trypsin + 0.5mg/mlDNAse I (Roche) for cortex samples. ..

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