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nu7741  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc nu7741
    Nu7741, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/advanblock+blocking+buffer/nu7741/pm35688134-239-183-184
    Average 90 stars, based on 1 article reviews
    nu7741 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Recombinant:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Blocking Assay:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Modification:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Filtration:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    High Molecular Weight:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Transfection:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A

    Staining:

    Article Title: Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ROS1 (phospho-Y2274) Thermo Fisher Scientific Cat#PA5-64608; RRID:AB_2663812 Mouse monoclonal anti-ROS1 Origene Cat#TA805734; RRID:AB_2627841 Mouse polyclonal anti-ZCCHC8 Abcam Cat#ab68739; RRID:AB_1271512 Mouse monoclonal anti-GFP Thermo Fisher Scientific Cat#MA5-15256; RRID:AB_10979281 Mouse monoclonal Anti-mCherry Thermo Fisher Scientific Cat#MA5-32977 Mouse monoclonal anti-beta-actin Thermo Fisher Scientific Cat#MA5-15739; RRID:AB_10979409 Sheep monoclonal anti-mouse IgG GE Healthcare Cat#NA931V; RRID:AB_772210 Bacterial and virus strains E. coli BL21CodonPlus(DE3)-RIL competent cells Agilent Technologies Cat#230245 E. coli E. cloni 10G competent cells Lucigen Cat#60106-2 Chemicals, peptides, and recombinant proteins AdvanBlock-Chemi blocking solution Advansta Cat#R-03726-E10 Adenosine 50(b,g-imido) triphosphate lithium salt hydrate Sigma Cat#A2647 GFP-Trap magnetic agarose Chromotek Cat#gtma-20; RRID:AB_2631358 IGEPAL CA-630 Sigma Cat#I8896 Iscove’s Modified Dulbecco’s medium Thermo Fisher Scientific Cat#12440053 Laemmli Sample buffer (43) Bio-Rad Cat#1610747 Magnesium chloride hexahydrate Sigma Cat#M9272 Tris(2-carboxyethyl)phosphine (TCEP) Soltec Ventures Cat#M115 RIPA Thermo Fisher Scientific Cat#89901 RNase inhibitor, human placenta New Engaland Biolabs Cat#M0307S Proteinase K New England Biolabs Cat#P8107S Superdex 200 Increase 10/300 GL GE Healthcare Cat#28990944 SuperSep Phos-tag (50 mmol/L), 12.5%, 17-well FUJIFILM Wako Cat#195-17991 SuperSignal West Dura Extended Duration substrate Thermo Fisher Scientific Cat#34075 TBE (103) Thermo Fisher Scientific Cat#AM9864 NU7741 StemCell Technologies Cat#74082 NuPAGE MES SDS Running Buffer (203) Thermo Fisher Scientific Cat#NP0002 NuPAGE LDS Sample Buffer (43) Thermo Fisher Scientific Cat#NP0007 Novex TBE gel, 20% Thermo Fisher Scientific Cat#EC63152BOX Novex TBE gel, 4–40% Thermo Fisher Scientific Cat#EC62252BOX NuPage 4–12% Bis-Tris protein gels Thermo Fisher Scientific Cat#NP0322BOX NuPage Transfer Buffer (203) Thermo Fisher Scientific Cat#NP006 Ni-NTA agarose Qiagen Cat#30250 HiTrap Heparin HP GE Healthcare Cat#17040601 HiLoad 26/600 Superdex 200 pg GE Healthcare Cat#289289336 Ulp1 protease In-house N/A Critical commercial assays Expi293 Expression System Kit Thermo Fisher Scientific Cat#A14635 Gel filtration calibration kit high molecular weight GE Healthcare Cat#28403842 iBind Flex solution kit Thermo Fisher Scientific Cat#SLF2020 Lipofectamine 3000 transfection reagents Thermo Fisher Scientific Cat#L3000001 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher Scientific Cat#L34955 (Continued on next page) e1 Cell 185, 2132–2147.e1–e11, June 9, 2022 .. (gel-based assay bottom strand for duplex with 30 20-nt poly(A) overhang) IDT N/A GCGUCUUUACGGUGCUUpyAAAAAAAAAAAAAAAAAAA (gel-based assay bottom strand for duplex with 20 pyrene modified uridine Upy) Dharmacon N/A



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