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rfp reporter  (Vector Biolabs)


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    Structured Review

    Vector Biolabs rfp reporter
    Rfp Reporter, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad+rfp/Ad-RFP/pm40134232-40-53-58
    Average 96 stars, based on 16 article reviews
    rfp reporter - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Knock-Out:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    In Vitro:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Control:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Expressing:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Marker:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Plasmid Preparation:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Construct:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Immunoprecipitation:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Western Blot:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Virus:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Recombinant:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Binding Assay:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Over Expression:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Infection:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..



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