annexin v fitc pi apoptosis analysis kit (Danaher Inc)
Structured Review

Annexin V Fitc Pi Apoptosis Analysis Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 7675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/pmc11711260-116-7-21?v=Danaher+Inc
Average 99 stars, based on 7675 article reviews
Images
1) Product Images from "miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway"
Article Title: miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway
Journal: Molecular Biotechnology
doi: 10.1007/s12033-024-01078-w
Figure Legend Snippet: MiR-324-3p suppresses the activation of transforming growth factor (TGF)-β1- induced LX-2 cells in vitro. A Hepatic stellate cells (HSCs) were transfected with miR-324-3p mimic, and expression of the miR-324-3p was determined by real-time-quantitative polymerase chain reaction (RT-qPCR). B RT-qPCR to analyze the miR-324-3p expression in HSCs transfected with the miR-324-3p inhibitor. C Cell counting kit-8 (CCK-8) to analyze the proliferation of transforming growth factor (TGF)-β1-induced LX-2 cells with indicated treatment. D Flow cytometry to detect the cycle and apoptosis of TGF-β1-induced LX-2 cells. E , G Western blot (WB) assay and RT-qPCR to evaluate α-smooth muscle actin (α-SMA) and Vimentin expression in transfected HSC cells. F , H The α-SMA, and Vimentin levels in transfected cells were analyzed by WB assay as well as RT-qPCR. * P < 0.05 and ** P < 0.01 vs . Control group; # P < 0.05 and ## P < 0.01 vs . NC + TGF-β1 group
Techniques Used: Activation Assay, In Vitro, Transfection, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Cell Counting, CCK-8 Assay, Flow Cytometry, Western Blot, Control
