ez dna methylation gold tm kit (Zymo Research)
Zymo Research is a verified supplier 
Zymo Research manufactures this product 
99
Structured Review
Zymo Research
ez dna methylation gold tm kit
Ez Dna Methylation Gold Tm Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/pmc05354504-811-18-23?v=Zymo+Research
Average 99 stars, based on 8209 article reviews
Ez Dna Methylation Gold Tm Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/pmc05354504-811-18-23?v=Zymo+Research
Average 99 stars, based on 8209 article reviews
ez dna methylation gold tm kit - by Bioz Stars,
2026-08
99/100 stars
Images
1) Product Images from "O -Linked N -Acetylglucosamine ( O -GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1 * "
Article Title: O -Linked N -Acetylglucosamine ( O -GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M116.763201
Figure Legend Snippet: MYBL1 was epigenetically regulated by O-GlcNAc. A, presence of CpG islands was analyzed around TSS site (−1500 to +350) of MYBL1 using the CpG Island Searcher. B, after the genomic DNA was isolated and bisulfate-treated, methylation-specific PCR for CDH1 (top) and MYBL1 (bottom) genes was performed in different colon tumor cells. The presence of a PCR product band in lanes M or U indicates methylated or unmethylated genes, respectively. M-positive, methylated human DNA standard used as a methylation-positive control. Data are representative of two independent experiments. C, tumor cells were treated with the demethylating reagent 5-aza-2′-deoxycytidine (5-Aza) at two different concentrations for 3 days, and total RNA was isolated and used for detection of transcript levels of MYBL1. For each transcript, the values were normalized to control (GAPDH) and expressed as mean ± S.D. from three independent experiments. D, after treatment with OGA inhibitor (TMG, 10 μm) for 24 h, tumor cells were collected for detection of transcripts of MYBL1 by qRT-PCR. E, after genomic DNA was isolated from tumor cells treated with OGA inhibitor (TMG, 10 μm) for 24 h and bisulfate-treated, methylation-specific PCR for the MYBL1 gene was performed. F, genomic DNA was isolated from both scrambled and OGT-shRNA transfected HT-29 cells, and methylation-specific PCR for the MYBL1 gene was performed. Images were representative from two independent experiments. *, p < 0.05; **, p < 0.01.
Techniques Used: Isolation, Methylation, Positive Control, Quantitative RT-PCR, shRNA, Transfection
