zymoclean gel dna recovery kit (Zymo Research)
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zymoclean gel dna recovery kit
Zymoclean Gel Dna Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 3820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/Zymoclean+Gel+DNA+Recovery+Kit/pmc12607995-237-29-34
Average 99 stars, based on 3820 article reviews
Zymoclean Gel Dna Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 3820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/Zymoclean+Gel+DNA+Recovery+Kit/pmc12607995-237-29-34
Average 99 stars, based on 3820 article reviews
zymoclean gel dna recovery kit - by Bioz Stars,
2026-11
99/100 stars
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Gibson Assembly:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Amplification:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Article Title: Functional identification of non-coding regulatory regions that drive breast and skin carcinogenesis. Article Snippet: Briefly, two micrograms of genomic DNA per sample were amplified using unique Illumina barcoded primer combinations (D501–510 and D701–712) with Q5 High-Fidelity DNA Polymerase (NEB) for 20–23 cycles. .. PCR products were resolved on a 2% agarose gel; the 200 bp band corresponding to the sgRNA amplicon was excised and purified using the Zymo Plasmid Preparation:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Polymerase Chain Reaction:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Article Title: Genomic insights into the metabolic complementarity of a synthetic marine consortium for hydrocarbon degradation in coastal sand microcosms. Article Snippet: Polymerase chain reaction (PCR) was carried out with Taq DNA polymerase (NZYTech, Lisbon, Portugal) using the following primers to amplify the complete 16S rRNA gene: 27F (AGA GTT TGATCMTGG CTC AG) and 1492R(I) (GGT TAC CTT GTT ACG ACT T). .. The PCR products were band-purified with a Article Title: Functional identification of non-coding regulatory regions that drive breast and skin carcinogenesis. Article Snippet: Briefly, two micrograms of genomic DNA per sample were amplified using unique Illumina barcoded primer combinations (D501–510 and D701–712) with Q5 High-Fidelity DNA Polymerase (NEB) for 20–23 cycles. .. PCR products were resolved on a 2% agarose gel; the 200 bp band corresponding to the sgRNA amplicon was excised and purified using the Zymo Article Title: Discovery and engineering of avian R2 retrotransposons for all-RNA-mediated targeted DNA integration in human cells. Article Snippet: To discover unknown retrotransposons for genomic integration in human cells, we searched 1,139 avian genomes, identified 159 avian R2 retrotransposons and characterized conserved/nonconserved sequence features in their proteins and untranslated region elements.. Engineered variants of these retrotransposons achieved up to 60% site-specific gene integration across human primary cells.. This work expands the avian R2 resource and provides additional tools for all-RNA-mediated genome editing. Gel Electrophoresis:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Agarose Gel Electrophoresis:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Article Title: Functional identification of non-coding regulatory regions that drive breast and skin carcinogenesis. Article Snippet: Briefly, two micrograms of genomic DNA per sample were amplified using unique Illumina barcoded primer combinations (D501–510 and D701–712) with Q5 High-Fidelity DNA Polymerase (NEB) for 20–23 cycles. .. PCR products were resolved on a 2% agarose gel; the 200 bp band corresponding to the sgRNA amplicon was excised and purified using the Zymo Article Title: Discovery and engineering of avian R2 retrotransposons for all-RNA-mediated targeted DNA integration in human cells. Article Snippet: To discover unknown retrotransposons for genomic integration in human cells, we searched 1,139 avian genomes, identified 159 avian R2 retrotransposons and characterized conserved/nonconserved sequence features in their proteins and untranslated region elements.. Engineered variants of these retrotransposons achieved up to 60% site-specific gene integration across human primary cells.. This work expands the avian R2 resource and provides additional tools for all-RNA-mediated genome editing. Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish Article Snippet: .. The target DNA fragments, ranging from 250 to 400 bp, were isolated by excising the corresponding bands from a 1.5% agarose gel and were purified using a Gel Extraction:Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing. Article Snippet: .. For Gibson assembly, relevant DNA segments were amplified from plasmid templates by PCR with Q5 high-fidelity 2× master mix (New England Biolabs), using primers that were commercially sourced from Integrated DNA Technologies, and PCR products were separated by gel electrophoresis on a 1% agarose gel run at 120 V for 30 min before gel extraction with the Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function Article Snippet: .. Chromatin Immunoprecipitation:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Qubit:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Staining:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Transferring:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Stripping Membranes:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Electrophoresis:Article Title: Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. Article Snippet: .. ChIP samples (from Support Protocol 3) 10 mM Tris·HCl, pH 7.5 (Current Protocols, 2006) Ovation Ultralow kit (Tecan, cat. no. 0344NB-32) 32-plex adapter plate (Tecan, cat. no. S02317-FG) High-sensitivity Qubit kit (Thermo Fisher Scientific, cat. no. Q32854) 50 bp DNA ladder (Invitrogen, cat. no. 10488099) 2% E-Gel agarose gel with SYBR Gold Safe Stain (Invitrogen, cat. no. G402022) 5% Purification:Article Title: Mosquito fauna (Diptera: Culicidae) in the Ria Celestún Biosphere Reserve (RCBR) with a new record for Yucatán, México Article Snippet: Thermal cycling conditions consisted of an initial denaturation at 95 °C for 5 min, hybridization at 35 cycles at 95 °C for 1 min, 46 °C for 1 min, and 72 °C for 1 min, followed by final elongation at 72 °C for 5 min. For ITS amplification, the cycling conditions included an initial denaturation at 94 °C for 5 min, followed by 35 cycles of 94 °C for 30 s, 50 °C for 1 min, and 72 °C for 1 min, with a final extension at 72 °C for 5 min. Amplicons were visualized on 1.5% agarose gels with 0.5 μg/mL ethidium bromide and visualized on a Bio-Rad Gel Doc XR+ with Image Lab software (Bio-Rad, Hercules, CA, USA). .. Bands were cut from the gel and purified using a Article Title: Functional identification of non-coding regulatory regions that drive breast and skin carcinogenesis. Article Snippet: Briefly, two micrograms of genomic DNA per sample were amplified using unique Illumina barcoded primer combinations (D501–510 and D701–712) with Q5 High-Fidelity DNA Polymerase (NEB) for 20–23 cycles. .. PCR products were resolved on a 2% agarose gel; the 200 bp band corresponding to the sgRNA amplicon was excised and purified using the Zymo Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish Article Snippet: .. The target DNA fragments, ranging from 250 to 400 bp, were isolated by excising the corresponding bands from a 1.5% agarose gel and were purified using a DNA Synthesis:Article Title: Genomic insights into the metabolic complementarity of a synthetic marine consortium for hydrocarbon degradation in coastal sand microcosms. Article Snippet: Polymerase chain reaction (PCR) was carried out with Taq DNA polymerase (NZYTech, Lisbon, Portugal) using the following primers to amplify the complete 16S rRNA gene: 27F (AGA GTT TGATCMTGG CTC AG) and 1492R(I) (GGT TAC CTT GTT ACG ACT T). .. The PCR products were band-purified with a Sequencing:Article Title: Genomic insights into the metabolic complementarity of a synthetic marine consortium for hydrocarbon degradation in coastal sand microcosms. Article Snippet: Polymerase chain reaction (PCR) was carried out with Taq DNA polymerase (NZYTech, Lisbon, Portugal) using the following primers to amplify the complete 16S rRNA gene: 27F (AGA GTT TGATCMTGG CTC AG) and 1492R(I) (GGT TAC CTT GTT ACG ACT T). .. The PCR products were band-purified with a Isolation:Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish Article Snippet: .. The target DNA fragments, ranging from 250 to 400 bp, were isolated by excising the corresponding bands from a 1.5% agarose gel and were purified using a |
