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depolymerization of the host s actin cytoskeleton  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc depolymerization of the host s actin cytoskeleton
    Summary of EV-mediated effect in viral pathogenesis.
    Depolymerization Of The Host S Actin Cytoskeleton, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+cytoskeleton+depolymerization/Actin+Polymerization+Biochem+Kit/pmc07589806-3-0-5
    Average 95 stars, based on 179 article reviews
    depolymerization of the host s actin cytoskeleton - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Extracellular Vesicles in the Pathogenesis of Viral Infections in Humans"

    Article Title: Extracellular Vesicles in the Pathogenesis of Viral Infections in Humans

    Journal: Viruses

    doi: 10.3390/v12101200

    Summary of EV-mediated effect in viral pathogenesis.
    Figure Legend Snippet: Summary of EV-mediated effect in viral pathogenesis.

    Techniques Used: Concentration Assay, Transmission Assay, Inhibition, Derivative Assay, Infection, Activation Assay, Binding Assay, Expressing, Generated, Cell Function Assay, Activity Assay, Permeability, Plasmid Preparation, Blocking Assay, Transferring, Functional Assay

    Related Articles

    Proximity Ligation Assay:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.

    In Situ:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.

    Fluorescence:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.

    Phospho-proteomics:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.

    Luciferase:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.

    Kinase Assay:

    Article Title: Legionella effector RavH is a tandem WH2-like domain–containing PI(3)P-dependent actin nucleator
    Article Snippet: letcher & Pollard, 1980 ). Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl 2 , 0.2 mM ATP, and 0.1 mM DTT). Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates. Reactions were started by adding actin to a mix of all other components a

    Article Title: Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator.
    Article Snippet: Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).Purified actin was stored in G-actin buffer (2 mM Tris–HCl (pH 8.0), 0.2 mM CaCl2, 0.2 mM ATP, and 0.1 mM DTT).. Actin Polymerization Biochem Kit (BK003) was purchased from Cytoskeleton Inc. Actin polymerization assays were performed in 200 μl reactions using 96-well black polystyrene assay plates.. Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).Reactions were started by adding actin to a mix of all other components and 10X actin polymerization buffer (500 mM KCl, 20 mM MgCl2, 0.05 M guanidine carbonate, and 10 mM ATP).

    Article Title: Evolutionary divergence V-ATPase function in macropinocytic cup remodeling
    Article Snippet: For quantitative assessment of GST-V1B induced actin polymerization, the actin polymerization Biochem Kit (BK003, Cytoskeleton, USA) was used as per the manufacturer’s instructions.. Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.Briefly, pyrene labelled G-actin (0.4 mg/mL) was prepared in general actin buffer supplemented with 0.2 mM ATP and equilibrated on ice for 1 hour.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections.
    Article Snippet: F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.F-actin co-sedimentation assay F-actin co-sedimentation assay was performed as described previously, with minor modifications.. Briefly, 2mg/ml actin from rabbit muscle (BK003, Cytoskeleton) was polymerized for 60 min at room temperature in 10× polymerization buffer containing 0.2 M Tris-HCl (pH 7.5), 1 M KCl, 20 mM MgCl2, and 1 mM dithiothreitol.. Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.Purified cofilin1 protein (5 μg of either wild type or mutants) was incubated with 10 μg of F-actin in 200 μl of 10mM Tris-HCl (pH 8.0) for 30min at room temperature.

    Article Title: MELK inhibition disrupts actin cytoskeleton and broadly restricts human coronavirus infections
    Article Snippet: Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.Steady-Glo luciferase assay (catalog no. E2520) and ADP-GloTM Kinase Assay (catalog no. V6930) were obtained from Promega.. SiR-actin (catalog no. CY-SC001) and the Actin Polymerization Biochem Kit (catalog no. BK003) were purchased from Cytoskeleton.. Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.Alexa FluorTM 647 phalloidin (catalog no. A22287) was purchased from Invitrogen.



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    Image Search Results


    Summary of EV-mediated effect in viral pathogenesis.

    Journal: Viruses

    Article Title: Extracellular Vesicles in the Pathogenesis of Viral Infections in Humans

    doi: 10.3390/v12101200

    Figure Lengend Snippet: Summary of EV-mediated effect in viral pathogenesis.

    Article Snippet: Depolymerization of the host’s actin cytoskeleton , Enhancement , Coxsackievirus B1 , Increased intracellular calcium concentration , Increased non-lytic viral spread , [ ] .

    Techniques: Concentration Assay, Transmission Assay, Inhibition, Derivative Assay, Infection, Activation Assay, Binding Assay, Expressing, Generated, Cell Function Assay, Activity Assay, Permeability, Plasmid Preparation, Blocking Assay, Transferring, Functional Assay