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Enzo Biochem fluorogenic substrate ac-devd-afc
Fluorogenic Substrate Ac Devd Afc, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+fluorogenic+substrate/fluorogenic+substrate+ac+rlr+amc/pmc09130777-83-9-12
Average 90 stars, based on 1 article reviews
fluorogenic substrate ac-devd-afc - by Bioz Stars, 2026-10
90/100 stars

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Article Title: BCL2 Regulates Differentiation of Intestinal Fibroblasts.
Article Snippet: Caspase-3/7 activity was quantified using the fluorogenic Ac-DEVD-AMC substrate (Enzo Life Sciences, Farmingdale, NY, USA).

Article Title: Biobran/MGN-3, an Arabinoxylan Rice Bran, Protects against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): An In Vitro and In Silico Study
Article Snippet: Reactions were triggered by adding the fluorogenic substrate Arg-Leu-Arg-Gly-Gly-AMC (Enzo Biochem, USA) (RLRGG-AMC, 10 μL of 250 μM) to buffer A, forcefully shaking for 30 s, and incubating for 6 min. Next, 10 μL acetic acid (0.5 M) was used to halt the reactions, the solution was shaken for 30 s, and the fluorescence emission intensity was measured (wavelength of excitation: 360 nm; wavelength of emission: 460 nm).

Article Title: AIM2 sensors mediate immunity to Plasmodium infection in hepatocytes.
Article Snippet: In short, 8 × 105 hepatocytes from 24 h infected or uninfected cultures were lysed and the supernatants were assayed for residual Caspase-1 activity by its ability to cleave the fluorogenic substrate Z-YVAD-AFC (Enzo) by incubating at 37 °C for 60 min.

Article Title: Chemical Optimization of Selective Pseudomonas aeruginosa LasB Elastase Inhibitors and Their Impact on LasB-Mediated Activation of IL-1β in Cellular and Animal Infection Models.
Article Snippet: Activity against human neutrophil elastase (HNE, Enzo Life Sciences) was assessed in 100 mM HEPES pH 7.5, 500 mM NaCl, 0.01% of Triton X-100 using 100 μM fluorogenic substrate MeOSuc-AAPV-AMC (Enzo Life Sciences).

Article Title: Curcumin Sensitizes Kidney Cancer Cells to TRAIL-Induced Apoptosis via ROS Mediated Activation of JNK-CHOP Pathway and Upregulation of DR4
Article Snippet: The activity of three of main caspases; caspase 8, -9, and -3/7 were quantified using specific fluorogenic AC-LETD-AFC, AC-LEHD-AFC and AC-DEVD-AFC substrates (ENZO, Exeter, UK) specific for these caspases, respectively.

Article Title: Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor Microenvironment
Article Snippet: 90 μl substrate solution were added (55 μM of fluorogenic substrate Ac-DEVD-AFC (Enzo, #ALX-260-032-M005), 50 mM HEPES, 0.1% [w/v] CHAPS, 1% [w/v] sucrose, 10 mM DTT, pH 7.5).

Incubation:

Article Title: Repurposing 1,2,4-oxadiazoles as SARS-CoV-2 PLpro inhibitors and investigation of their possible viral entry blockade potential.
Article Snippet: .. Reactions were initiated by the addition of a fluorogenic substrate, Arg-Leu-Arg-Gly-Gly-AMC (Enzo Biochem, USA) (RLRGGAMC, 10 μl of 250 μM) in buffer A, shaken vigorously for 30 s, and then incubated for 6 min. ..



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