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Becton Dickinson ac-devd-afc caspase-3 fluorogenic substrate
Ac Devd Afc Caspase 3 Fluorogenic Substrate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+fluorogenic+substrate/ac+devd+afc/pmc06906283-44-3-10
Average 90 stars, based on 1 article reviews
ac-devd-afc caspase-3 fluorogenic substrate - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Matrix metalloproteinase-10 promotes tumor progression through regulation of angiogenic and apoptotic pathways in cervical tumors
Article Snippet: Aliquots of 6 mg of crude cell lysate were incubated with caspase-3 substrate Ac-DEVD-AMC (Pharmingen, San Diego, CA) at 37°C for 30 min.

Article Title: The polarity protein Par3 coordinates positively self-renewal and negatively invasiveness in glioblastoma
Article Snippet: Transfected cells with control and Par3 siRNAs were lysed in 0.5% Triton X-100, 5 mM Tris-HCl, pH 8.0, 20 mM EDTA, for caspase-3 activity, a reaction containing 20−40 μg protein extract and 20 μM fluorogenic caspase-3 substrate (Ac-DEVD-AMC, excitation at 380 nm, emission at 440 nm; BD Biosciences, Stockholm, Sweden) in 40 mM HEPES, pH 7.5, 20% glycerol, 4 mM dithiothreitol buffer, was incubated for 2 h in the dark at 37 °C, and analyzed fluorimetrically.

Article Title: BMP9 Promotes an Epithelial Phenotype and a Hepatocyte-like Gene Expression Profile in Adult Hepatic Progenitor Cells
Article Snippet: Lysates were clarified by centrifugation at 13,000× g for 10 min. A reaction mixture containing 25 μL cell lysate, 325 μL assay buffer (20 mM HEPES pH 7.5, 10% glycerol, 2 mM dithiothreitol) and 20 μM caspase-3 substrate (BD Biosciences, Franklin Lakes, NJ, USA) was incubated for 2 h at 37 °C.

Article Title: The balance between two isoforms of LEF-1 regulates colon carcinoma growth
Article Snippet: Caspase-3 fluorogenic substrate, Ac-DEVD-AFC, came from BD Biosciences (San Jose, CA, USA).

Article Title: Uncovering a Novel Functional Interaction Between Adult Hepatic Progenitor Cells, Inflammation and EGFR Signaling During Bile Acids-Induced Injury
Article Snippet: Reaction mixture contained 12.5 μL cell lysate, 162.5 μL assay buffer (20mM HEPES pH 7.5, 10% glycerol, 2mM DTT) and 20μM caspase-3 substrate (Ac-DEVD-AMC, from BD Biosciences).

Article Title: ERBB and P-glycoprotein inhibitors break resistance in relapsed neuroblastoma models through P-glycoprotein.
Article Snippet: Ten millimolar DTT and 50 μM caspase-3/7 fluorogenic substrate (AcDEVD-AFC, #556574, BD PharmingenTM, BD Biosciences) were added to 10 μg of protein in reaction buffer (#JM-1068-20, MBL International, Woburn, MA, USA).

Article Title: Differential effects of hypoxia on etoposide-induced apoptosis according to the cancer cell lines
Article Snippet: The protein concentration was measured and 5 μg completed to 50 μl with lysis buffer were mixed with 13 μM Ac-DEVD-AFC (BD Pharmingen) and 50 μl reaction buffer (40 mM PIPES, pH 7.2, 200 mM NaCl, 2 mM EDTA, 0.2 % CHAPS, 0.10 % sucrose and 10 mM dithiothreitol).

Incubation:

Article Title: Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice
Article Snippet: .. For each reaction, caspase 3 synthetic fluorogenic tetrapeptide substrate (Ac-DEVD-AMC) was added (BD Pharmingen) and then the tissue lysate; after that samples were incubated for one hour. ..



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