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verikine hs mouse interferon beta serum elisa kit  (PBL Assay)


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    PBL Assay verikine hs mouse interferon beta serum elisa kit
    Non-phagocytic resolution phase macrophages express IFN-β. a , b Male mice were injected intraperitoneally with zymosan A (1 mg/mouse) followed by an injection of PKH2-PCL at 62 h. After 4 h, the peritoneal cells were recovered and immuno-stained for F4/80 and CD11b. Then, F4/80 + macrophages were sorted based on the extent of PKH2-PCL acquisition (PKH2 + /− populations; >98% purity) using the FACSAria III sorter (illustrated in ( a ). The collected cells were immediately used for RNA extraction (with RNA integrity value above 7.5), and a gene expression microarray analysis was performed using Illumina hiSeq 2500. Differential gene expression analysis and gene ontology (GO) enrichment were performed for genes that were significantly upregulated ( b , left panel) or downregulated ( b , right panel) in non-phagocytic/satiated (PKH2-PCL lo ) macrophages in comparison to phagocytic (PKH2-PCL hi ) ones. The results indicate the statistical significance of the GO term and the percentage of enrichment is presented. c – e Expression of IFN-β and ISG15 in sorted satiated and phagocytic macrophages. Representative results ( c ) and mean ± SEM ( d , e ) for three independent experiments. * P < 0.05 (Student’s t test). g , h Peritoneal exudates were collected from unchallenged mice (0 h) or following peritonitis for 4–96 h. IFN-β content in cell-free fluids was determined by <t>ELISA</t> ( f ). Results are mean ± SEM from three (24, 72, 96 h) or four (0, 4, 48 h) mice. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Alternatively, resolution phase macrophages were recovered 66 h post peritonitis initiation (PPI) and incubated with TGF-β (5 ng/ml), poly (I:C) (4 μg/ml) or apoptotic cells (AC, at a ratio of 1:5) for 24 h. Culture supernatants were then collected and IFN-β content was measured ( g ). Culture media from apoptotic cells served as control. Results are representative from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Source data are provided as a Source Data file
    Verikine Hs Mouse Interferon Beta Serum Elisa Kit, supplied by PBL Assay, used in various techniques. Bioz Stars score: 96/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab+microarray+express+buffer+kit/Mouse+Interferon+Beta/pmc06677895-350-23-30
    Average 96 stars, based on 644 article reviews
    verikine hs mouse interferon beta serum elisa kit - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation"

    Article Title: IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation

    Journal: Nature Communications

    doi: 10.1038/s41467-019-10903-9

    Non-phagocytic resolution phase macrophages express IFN-β. a , b Male mice were injected intraperitoneally with zymosan A (1 mg/mouse) followed by an injection of PKH2-PCL at 62 h. After 4 h, the peritoneal cells were recovered and immuno-stained for F4/80 and CD11b. Then, F4/80 + macrophages were sorted based on the extent of PKH2-PCL acquisition (PKH2 + /− populations; >98% purity) using the FACSAria III sorter (illustrated in ( a ). The collected cells were immediately used for RNA extraction (with RNA integrity value above 7.5), and a gene expression microarray analysis was performed using Illumina hiSeq 2500. Differential gene expression analysis and gene ontology (GO) enrichment were performed for genes that were significantly upregulated ( b , left panel) or downregulated ( b , right panel) in non-phagocytic/satiated (PKH2-PCL lo ) macrophages in comparison to phagocytic (PKH2-PCL hi ) ones. The results indicate the statistical significance of the GO term and the percentage of enrichment is presented. c – e Expression of IFN-β and ISG15 in sorted satiated and phagocytic macrophages. Representative results ( c ) and mean ± SEM ( d , e ) for three independent experiments. * P < 0.05 (Student’s t test). g , h Peritoneal exudates were collected from unchallenged mice (0 h) or following peritonitis for 4–96 h. IFN-β content in cell-free fluids was determined by ELISA ( f ). Results are mean ± SEM from three (24, 72, 96 h) or four (0, 4, 48 h) mice. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Alternatively, resolution phase macrophages were recovered 66 h post peritonitis initiation (PPI) and incubated with TGF-β (5 ng/ml), poly (I:C) (4 μg/ml) or apoptotic cells (AC, at a ratio of 1:5) for 24 h. Culture supernatants were then collected and IFN-β content was measured ( g ). Culture media from apoptotic cells served as control. Results are representative from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Source data are provided as a Source Data file
    Figure Legend Snippet: Non-phagocytic resolution phase macrophages express IFN-β. a , b Male mice were injected intraperitoneally with zymosan A (1 mg/mouse) followed by an injection of PKH2-PCL at 62 h. After 4 h, the peritoneal cells were recovered and immuno-stained for F4/80 and CD11b. Then, F4/80 + macrophages were sorted based on the extent of PKH2-PCL acquisition (PKH2 + /− populations; >98% purity) using the FACSAria III sorter (illustrated in ( a ). The collected cells were immediately used for RNA extraction (with RNA integrity value above 7.5), and a gene expression microarray analysis was performed using Illumina hiSeq 2500. Differential gene expression analysis and gene ontology (GO) enrichment were performed for genes that were significantly upregulated ( b , left panel) or downregulated ( b , right panel) in non-phagocytic/satiated (PKH2-PCL lo ) macrophages in comparison to phagocytic (PKH2-PCL hi ) ones. The results indicate the statistical significance of the GO term and the percentage of enrichment is presented. c – e Expression of IFN-β and ISG15 in sorted satiated and phagocytic macrophages. Representative results ( c ) and mean ± SEM ( d , e ) for three independent experiments. * P < 0.05 (Student’s t test). g , h Peritoneal exudates were collected from unchallenged mice (0 h) or following peritonitis for 4–96 h. IFN-β content in cell-free fluids was determined by ELISA ( f ). Results are mean ± SEM from three (24, 72, 96 h) or four (0, 4, 48 h) mice. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Alternatively, resolution phase macrophages were recovered 66 h post peritonitis initiation (PPI) and incubated with TGF-β (5 ng/ml), poly (I:C) (4 μg/ml) or apoptotic cells (AC, at a ratio of 1:5) for 24 h. Culture supernatants were then collected and IFN-β content was measured ( g ). Culture media from apoptotic cells served as control. Results are representative from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005 (Tukey’s HSD). Source data are provided as a Source Data file

    Techniques Used: Injection, Staining, RNA Extraction, Gene Expression, Microarray, Comparison, Expressing, Enzyme-linked Immunosorbent Assay, Incubation, Control

    IFN-β favors macrophage reprogramming during the resolution of inflammation. a – d Macrophages were recovered from peritoneal exudates of male Ifnb +/+ or Ifnb − / − mice at 48–66 h PPI and cultured with LPS (1 μg/ml) for 24 h. Culture supernatants were then collected and levels of IL-10 ( a ), IL-6 ( b ), IL-12 ( c ) and CCL3 ( d ) were determined by selective ELISAs. Results are means ± SEM from four independent experiments. * P < 0.05, *** P < 0.005 (Tukey’s HSD). e – f Macrophages were recovered from peritoneal exudates of Ifnb +/+ mice at 48–66 h PPI and cultured with mouse IFN-β or IFN-α (20 ng/ml each) for 48 h. Then, culture supernatants were collected and levels of IL-10 ( e ) and IL-12 ( f ) were determined by ELISA. Results are means ± SEM (n = 4). *** P < 0.005 (Tukey’s HSD). g Macrophages were recovered from peritoneal exudates of Ifnb +/+ mice 48–66 h PPI and incubated with IFN-β (20 ng/ml) for 48 h. The cells were then immunostained for F4/80 and CD11b and the percentage of CD11b low macrophages was determined by flow cytometry. Results are means ± SEM from three independent experiments. *** P < 0.005 (Tukey’s HSD). h – i Mice undergoing peritonitis were treated with IFN-β (20 ng/mouse, i.p.) or vehicle at 24 h PPI. Peritoneal macrophages were collected at 48 h PPI, lysed and immunoblotted for 12/15-LO, arginase 1, ISG15 and GAPDH. Representative blots ( h ) and densitometry analysis (means ± SEM) ( i ) for three independent experiments. * P < 0.05 (Student’s t test). Source data are provided as a Source Data file
    Figure Legend Snippet: IFN-β favors macrophage reprogramming during the resolution of inflammation. a – d Macrophages were recovered from peritoneal exudates of male Ifnb +/+ or Ifnb − / − mice at 48–66 h PPI and cultured with LPS (1 μg/ml) for 24 h. Culture supernatants were then collected and levels of IL-10 ( a ), IL-6 ( b ), IL-12 ( c ) and CCL3 ( d ) were determined by selective ELISAs. Results are means ± SEM from four independent experiments. * P < 0.05, *** P < 0.005 (Tukey’s HSD). e – f Macrophages were recovered from peritoneal exudates of Ifnb +/+ mice at 48–66 h PPI and cultured with mouse IFN-β or IFN-α (20 ng/ml each) for 48 h. Then, culture supernatants were collected and levels of IL-10 ( e ) and IL-12 ( f ) were determined by ELISA. Results are means ± SEM (n = 4). *** P < 0.005 (Tukey’s HSD). g Macrophages were recovered from peritoneal exudates of Ifnb +/+ mice 48–66 h PPI and incubated with IFN-β (20 ng/ml) for 48 h. The cells were then immunostained for F4/80 and CD11b and the percentage of CD11b low macrophages was determined by flow cytometry. Results are means ± SEM from three independent experiments. *** P < 0.005 (Tukey’s HSD). h – i Mice undergoing peritonitis were treated with IFN-β (20 ng/mouse, i.p.) or vehicle at 24 h PPI. Peritoneal macrophages were collected at 48 h PPI, lysed and immunoblotted for 12/15-LO, arginase 1, ISG15 and GAPDH. Representative blots ( h ) and densitometry analysis (means ± SEM) ( i ) for three independent experiments. * P < 0.05 (Student’s t test). Source data are provided as a Source Data file

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay, Incubation, Flow Cytometry

    Related Articles

    Recombinant:

    Article Title: AAV immuno-gene therapy platform delivering vectorized cytokines defines a new modality for high-grade glioma treatment
    Article Snippet: .. Human PBMCs were treated with either recombinant human IFNβ (PBL Cat#11415-1) or media control and placed in an incubator (37°C, 5% CO 2 , with saturating humidity) for 24 ± 1 h. Mouse PBMCs were treated with recombinant mouse IFNβ (PBL Cat#12405-1 Lot:7573R), recombinant human IFNβ, or media control and placed in an incubator (37°C, 5% CO 2 , with saturating humidity) for 24 ± 1 h. At the time of collection, plates were spun in a swinging bucket rotor centrifuge (350 × g , 5 min). .. Supernatant was aspirated, and RNA extractions from cells were performed using the PureLink Pro 96 RNA Purification Kit (Invitrogen Cat#12173011A).

    Control:

    Article Title: AAV immuno-gene therapy platform delivering vectorized cytokines defines a new modality for high-grade glioma treatment
    Article Snippet: .. Human PBMCs were treated with either recombinant human IFNβ (PBL Cat#11415-1) or media control and placed in an incubator (37°C, 5% CO 2 , with saturating humidity) for 24 ± 1 h. Mouse PBMCs were treated with recombinant mouse IFNβ (PBL Cat#12405-1 Lot:7573R), recombinant human IFNβ, or media control and placed in an incubator (37°C, 5% CO 2 , with saturating humidity) for 24 ± 1 h. At the time of collection, plates were spun in a swinging bucket rotor centrifuge (350 × g , 5 min). .. Supernatant was aspirated, and RNA extractions from cells were performed using the PureLink Pro 96 RNA Purification Kit (Invitrogen Cat#12173011A).

    Infection:

    Article Title: A dominant role of cell death in limiting Chandipura virus propagation at cell-saturating high multiplicity of infection
    Article Snippet: .. The abundance of IFNβ in the culture supernatant of infected MEFs was determined using a mouse IFNβ ELISA kit (PBL Assay Science, Piscataway, NJ) adhering to manufacturer’s protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A dominant role of cell death in limiting Chandipura virus propagation at cell-saturating high multiplicity of infection
    Article Snippet: .. The abundance of IFNβ in the culture supernatant of infected MEFs was determined using a mouse IFNβ ELISA kit (PBL Assay Science, Piscataway, NJ) adhering to manufacturer’s protocol. ..

    Article Title: STING-dependent peripheral inflammaging drives neurodegeneration via extracellular vesicles.
    Article Snippet: Dloop primer mtDNA Forward: 5′-AATCTACCATCCTCCGTGAAACC-3′ mtDNA Reverse: 5′-TCAGTTTAGCTACCCCCAAGTTTAA-3′ Actb primer nuDNA Forward: 5′-TTT GCT CCT GGG CCT CCA AGT T-3′ nuDNA Reverse: 5′-AGC CCG TGA CTG CCA CAA ATC A-3′ Measurement of IFN-β protein levels by ELISA IFN-β protein levels in the plasma of Parkinson’s disease patients and healthy donors were measured using the Human IFN-β DuoSet ELISA kit (R&D Systems, DY814-05), following the manufacturer’s protocol. .. Similarly, IFN-β protein levels in the plasma of Lrrk2WT and Lrrk2GoF mice were measured using the Mouse IFN-β High Sensitivity ELISA Kit (PBL Assay Science, 42410). .. Levels of cGAMP in the plasma of Parkinson’s disease patients, Healthy donors, Lrrk2WT and Lrrk2GoF mice, as well as cell lysate of Lrrk2WT and Lrrk2GoF MEFs were measured by Cayman Chemical 2′ 3′-cGAMP ELISA Kit (501700) according to manufacturer’s protocol.

    Clinical Proteomics:

    Article Title: STING-dependent peripheral inflammaging drives neurodegeneration via extracellular vesicles.
    Article Snippet: Dloop primer mtDNA Forward: 5′-AATCTACCATCCTCCGTGAAACC-3′ mtDNA Reverse: 5′-TCAGTTTAGCTACCCCCAAGTTTAA-3′ Actb primer nuDNA Forward: 5′-TTT GCT CCT GGG CCT CCA AGT T-3′ nuDNA Reverse: 5′-AGC CCG TGA CTG CCA CAA ATC A-3′ Measurement of IFN-β protein levels by ELISA IFN-β protein levels in the plasma of Parkinson’s disease patients and healthy donors were measured using the Human IFN-β DuoSet ELISA kit (R&D Systems, DY814-05), following the manufacturer’s protocol. .. Similarly, IFN-β protein levels in the plasma of Lrrk2WT and Lrrk2GoF mice were measured using the Mouse IFN-β High Sensitivity ELISA Kit (PBL Assay Science, 42410). .. Levels of cGAMP in the plasma of Parkinson’s disease patients, Healthy donors, Lrrk2WT and Lrrk2GoF mice, as well as cell lysate of Lrrk2WT and Lrrk2GoF MEFs were measured by Cayman Chemical 2′ 3′-cGAMP ELISA Kit (501700) according to manufacturer’s protocol.



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