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zeiss axio vert a1 microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss axio vert a1 microscope
    Zeiss Axio Vert A1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 765 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a1+microscope/Microscope+stand+Axio+Vert%2EA1/pmc12856442-82-33-33
    Average 97 stars, based on 765 article reviews
    zeiss axio vert a1 microscope - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Macrophage FTO deficiency accelerates atherosclerosis via PACS2-mediated activation of the PPARγ lipid signaling pathway.
    Article Snippet: 211 Dil-OxLDL Uptake Assay 212 The macrophages were incubated with 20 μg/mL fluorescent-labeled oxidized 213 LDL (Dil-OxLDL) (Yiyuan Biotechnologies) for 6 h at 37 °C. .. After the cells were 214 washed with PBS to remove noninternalized particles, cellular Dil-OxLDL uptake 215 was visualized under a Zeiss Axio Vert.A1 fluorescence microscope and quantified by 216 measuring the mean fluorescence intensity (MFI) using ImageJ. .. 217 BODIPY Staining of Lipid Droplets 218 To visualize lipid droplets, the macrophages were incubated with 10 μM 219 BODIPY 493/503 (HY-W090090; MCE, New Jersey, USA) for 30 min at 37 °C in 220 the dark.


    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Cell viability within the hydrogel was assessed using a Live/Dead Viability/Cytotoxicity Kit (C2015S, Beyotime, China) following the manufacturer's instructions. .. ND7/23 cells were co-cultured with PDA-HP hydrogel for 48 h, after which the staining solution was applied, and samples were incubated at 37 °C for 15 min. Fluorescence images were acquired using a Zeiss Axio Vert.A1 microscope (Germany). .. MMP was assessed using the JC-1 fluorescent probe (C2006, Beyotime, China), which selectively accumulates in mitochondria and exhibits a shift in fluorescence emission depending on ΔΨm.

    Microscopy:

    Article Title: Macrophage FTO deficiency accelerates atherosclerosis via PACS2-mediated activation of the PPARγ lipid signaling pathway.
    Article Snippet: 211 Dil-OxLDL Uptake Assay 212 The macrophages were incubated with 20 μg/mL fluorescent-labeled oxidized 213 LDL (Dil-OxLDL) (Yiyuan Biotechnologies) for 6 h at 37 °C. .. After the cells were 214 washed with PBS to remove noninternalized particles, cellular Dil-OxLDL uptake 215 was visualized under a Zeiss Axio Vert.A1 fluorescence microscope and quantified by 216 measuring the mean fluorescence intensity (MFI) using ImageJ. .. 217 BODIPY Staining of Lipid Droplets 218 To visualize lipid droplets, the macrophages were incubated with 10 μM 219 BODIPY 493/503 (HY-W090090; MCE, New Jersey, USA) for 30 min at 37 °C in 220 the dark.


    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Cell viability within the hydrogel was assessed using a Live/Dead Viability/Cytotoxicity Kit (C2015S, Beyotime, China) following the manufacturer's instructions. .. ND7/23 cells were co-cultured with PDA-HP hydrogel for 48 h, after which the staining solution was applied, and samples were incubated at 37 °C for 15 min. Fluorescence images were acquired using a Zeiss Axio Vert.A1 microscope (Germany). .. MMP was assessed using the JC-1 fluorescent probe (C2006, Beyotime, China), which selectively accumulates in mitochondria and exhibits a shift in fluorescence emission depending on ΔΨm.

    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Intracellular ROS levels in ND7/23 cells were assessed using the cell-permeable probe DCFH-DA (S0033, Beyotime, China). .. Cells were seeded at 1.5 × 10 5 per well and allowed to stabilize for 24 h, followed by exposure to 100 μM T-BHP for 6 h. Cells were then incubated with 10 μM DCFH-DA at 37 °C for 30 min, washed twice with PBS, and immediately imaged using a Zeiss Axio Vert.A1 microscope (Germany). .. Green DCF fluorescence, proportional to ROS levels, was quantified using ImageJ software.

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: For cell counting and size, cells were trypsinized, resuspended in DMEM supplemented with 10% FBS and analyzed by automatic cell counter Luna II (Logos Biosystems). .. Cell morphology images were taken in an Axio Vert.A1 Microscope (Zeiss). ..

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: .. Images were taken using an Axio Vert.A1 Microscope (Zeiss). ..

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: .. Images were taken with Axio Vert.A1 Microscope (Zeiss) and 10 images per condition were quantified. .. Cellular lysates were prepared in 1x RIPA buffer containing protease and phosphatase inhibitor cocktails (4693159001 and 4906845001, Sigma-Aldrich).

    Imaging:


    Staining:

    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Cell viability within the hydrogel was assessed using a Live/Dead Viability/Cytotoxicity Kit (C2015S, Beyotime, China) following the manufacturer's instructions. .. ND7/23 cells were co-cultured with PDA-HP hydrogel for 48 h, after which the staining solution was applied, and samples were incubated at 37 °C for 15 min. Fluorescence images were acquired using a Zeiss Axio Vert.A1 microscope (Germany). .. MMP was assessed using the JC-1 fluorescent probe (C2006, Beyotime, China), which selectively accumulates in mitochondria and exhibits a shift in fluorescence emission depending on ΔΨm.

    Incubation:

    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Cell viability within the hydrogel was assessed using a Live/Dead Viability/Cytotoxicity Kit (C2015S, Beyotime, China) following the manufacturer's instructions. .. ND7/23 cells were co-cultured with PDA-HP hydrogel for 48 h, after which the staining solution was applied, and samples were incubated at 37 °C for 15 min. Fluorescence images were acquired using a Zeiss Axio Vert.A1 microscope (Germany). .. MMP was assessed using the JC-1 fluorescent probe (C2006, Beyotime, China), which selectively accumulates in mitochondria and exhibits a shift in fluorescence emission depending on ΔΨm.

    Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
    Article Snippet: Intracellular ROS levels in ND7/23 cells were assessed using the cell-permeable probe DCFH-DA (S0033, Beyotime, China). .. Cells were seeded at 1.5 × 10 5 per well and allowed to stabilize for 24 h, followed by exposure to 100 μM T-BHP for 6 h. Cells were then incubated with 10 μM DCFH-DA at 37 °C for 30 min, washed twice with PBS, and immediately imaged using a Zeiss Axio Vert.A1 microscope (Germany). .. Green DCF fluorescence, proportional to ROS levels, was quantified using ImageJ software.

    other:

    Article Title: Microfluidics-enabled proteomic profiling reveal iron-driven immune evasion by an antimicrobial-resistant pathogen.
    Article Snippet: To test filter channel heights (i.e., 1.4, 3.5 and 4.5 μm) for cell separation, 3 mL of macrophage cells (immortalized cell line derived from BALB/c mice, obtained from Geddes-McAlister lab) in 0.01 M PBS (∼1.5 × 10 6 cells/mL) was processed in each chip at 6 mL/h flow rate and observed with a microscope (ZEISS Axio Vert.A1).



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