c1000 touchtm thermal cycler (Bio-Rad)

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Bio-Rad c1000 touchtm thermal cycler
C1000 Touchtm Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+smart+array+48+arrayer/C1000+Touch+Thermal+Cycler+with+96%E2%80%93Deep+Well+Reaction+Module/pmc10184414-116-11-22
Average 97 stars, based on 4841 article reviews

c1000 touchtm thermal cycler - by Bioz Stars, 2026-09

97/100 stars

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Polymerase Chain Reaction:

Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs
Article Snippet: Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad). .. Droplet thresholds were manually set using QX Manager Software (Bio-Rad, Version 2.3) and processed in Excel (Microsoft).

Article Title: Intrinsic regulation of intestinal stem cell fate and homeostasis by Tet
Article Snippet: .. All PCR reactions were performed using the BioRad C1000 Touch Thermal cycler with a 96-deep Well Reaction Module. ..

Article Title: Mapping fibrotic microenvironments: single-cell and spatial profiling of Schistosoma mansoni-induced tissue fibrosis
Article Snippet: After collecting all tissue sections onto the Xenium slides, the tissues were baked in a 42°C oven for 3 h followed by overnight drying in a Falcon tube with desiccant (Merck, PN# 1.01972). .. The tissue deparaffinization was performed by baking the slides on the Xenium Thermocycler Adapter v2 (10x Genomics, PN# 1000739) on a PCR machine (Bio-Rad, PN# 1851197) at 60°C for 30 min, followed by two Xylene washes, serial ethanol washes (2 × 100%, 2 x 96%, 1 x 70%), and lastly the nuclease-free water wash. .. The Xenium slides were assembled into Xenium Cassette (10x Genomics, PN# 1000726) before proceeding to the Decrosslinking.

Single Cell:

Article Title: Single-cell transcriptomic and chromatin accessibility atlas of peripheral blood mononuclear cells reveals immune cell heterogeneity and breed-specific characteristics in Duroc and Meishan pigs.
Article Snippet: Vortexed Chromium Single Cell ATAC Gel Beads (10x Genomics; 2000132) and Partitioning Oil (10x Genomics; 220088) were also loaded onto the same Chromium Chip E and placing into a Chromium Single Cell Controller instrument (10x Genomics). .. Resulting single-cell GEMs were collected at the completion of the run (~7 min) and linear amplification was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; 1851197). .. Emulsions were coalesced using the Recovery Agent (10x Genomics; 220016), then subjected to Dynabeads (10x Genomics; PN-2000048) and SPRIselect reagent (Beckman Coulter; B23318) bead clean-ups.

Amplification:

Purification:

Article Title: Single-cell transcriptomics of bronchoalveolar lavage reveals divergent macrophage subpopulations and trajectories in interstitial lung disease
Article Snippet: Single-cell suspensions for samples pertaining to BAL_scRNA_sample# were loaded onto 10X Genomics Single Cell 3′ Chips along with the RT mastermix as per the manufacturer’s protocol for the Chromium Single Cell 3′ Library (v2, PN-120233; 10X Genomics), to generate single-cell gel beads in emulsion. .. RT was performed using a C1000 Touch Thermal Cycler with a Deep Well Reaction Module (Bio-Rad) as follows: 55°C for 2 h; 85°C for 5 min; hold 4°C. cDNA was recovered and purified with DynaBeads MyOne Silane Beads (catalog no. 37002D; Thermo Fisher Scientific) and SPRIselect beads (catalog no. B23318 ; Beckman Coulter). ..

Reverse Transcription:

Article Title: Longitudinal localization of leukaemic stem cells between the metaphysis and central marrow governs their behaviour.
Article Snippet: .. Reverse transcription and library preparation were performed on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). .. Amplified cDNA and final libraries were evaluated on an Agilent Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies).

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