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dual luciferase report array system  (Promega)

 
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    Structured Review

    Promega dual luciferase report array system
    GDF9 promoter activity analysis in HEK293T. The g.46547859C>T , g.46548061A>G , and g.46548326C>T SNPs were in LD of Mongolia sheep populations, designated LD-M1. <t>Luciferase</t> reporter assay used the recombinant plasmids pGL3-1533, pGL3-1292-C, pGL3-726-A, pGL3-460-C, pGL3-177, and pGL3-110, and the g.46548326C>T , g.46548061A>G , and g.46547859C>T SNPs of LD-M1 is located between −1292 and −726, −726 and −460, and −460 and −177 bp upstream, respectively, of GDF9 translation initiation site. Luciferase activities of different GDF9 promoter fragments are shown on the right panel. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01.
    Dual Luciferase Report Array System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+smart+array+48+arrayer/dual+luciferase+report+array+system/pmc07230991-130-14-19
    Average 90 stars, based on 1 article reviews
    dual luciferase report array system - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep"

    Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep

    Journal: Genes

    doi: 10.3390/genes11040375

    GDF9 promoter activity analysis in HEK293T. The g.46547859C>T , g.46548061A>G , and g.46548326C>T SNPs were in LD of Mongolia sheep populations, designated LD-M1. Luciferase reporter assay used the recombinant plasmids pGL3-1533, pGL3-1292-C, pGL3-726-A, pGL3-460-C, pGL3-177, and pGL3-110, and the g.46548326C>T , g.46548061A>G , and g.46547859C>T SNPs of LD-M1 is located between −1292 and −726, −726 and −460, and −460 and −177 bp upstream, respectively, of GDF9 translation initiation site. Luciferase activities of different GDF9 promoter fragments are shown on the right panel. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: GDF9 promoter activity analysis in HEK293T. The g.46547859C>T , g.46548061A>G , and g.46548326C>T SNPs were in LD of Mongolia sheep populations, designated LD-M1. Luciferase reporter assay used the recombinant plasmids pGL3-1533, pGL3-1292-C, pGL3-726-A, pGL3-460-C, pGL3-177, and pGL3-110, and the g.46548326C>T , g.46548061A>G , and g.46547859C>T SNPs of LD-M1 is located between −1292 and −726, −726 and −460, and −460 and −177 bp upstream, respectively, of GDF9 translation initiation site. Luciferase activities of different GDF9 promoter fragments are shown on the right panel. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01.

    Techniques Used: Activity Assay, Luciferase, Reporter Assay, Recombinant

    Effect of the LD-M1 on promoter activity of ovine GDF9 . ( a ) Luciferase activity results showed that the C allele at the g.46547859C>T SNP could decrease promoter activity than T allele. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01. ( b ) Prediction of transcription factor binding motif in conserved sequence including the g.46547859C>T SNP site.
    Figure Legend Snippet: Effect of the LD-M1 on promoter activity of ovine GDF9 . ( a ) Luciferase activity results showed that the C allele at the g.46547859C>T SNP could decrease promoter activity than T allele. Results are shown as mean ± SD and the data are representative of at least three independent assays. The statistically significant difference between groups was tested by independent sample t -test. * p < 0.05, ** p < 0.01. ( b ) Prediction of transcription factor binding motif in conserved sequence including the g.46547859C>T SNP site.

    Techniques Used: Activity Assay, Luciferase, Binding Assay, Sequencing

    Related Articles

    Luciferase:

    Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep
    Article Snippet: .. Firefly and Renilla luciferase activity levels were measured after 48 h transfection with a Dual Luciferase Report Array System (Promega, Madison, WI, USA) in a Varisskan Flash Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers’ protocols. ..

    Activity Assay:

    Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep
    Article Snippet: .. Firefly and Renilla luciferase activity levels were measured after 48 h transfection with a Dual Luciferase Report Array System (Promega, Madison, WI, USA) in a Varisskan Flash Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers’ protocols. ..

    Transfection:

    Article Title: Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep
    Article Snippet: .. Firefly and Renilla luciferase activity levels were measured after 48 h transfection with a Dual Luciferase Report Array System (Promega, Madison, WI, USA) in a Varisskan Flash Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers’ protocols. ..



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