antibody to upf1 (Novus Biologicals)
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antibody to upf1
Antibody To Upf1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NBP1-05967/RENT1%2FUPF1%2FhUPF1+Antibody/pm38982067-386-9-13
Average 93 stars, based on 2 article reviews
Antibody To Upf1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NBP1-05967/RENT1%2FUPF1%2FhUPF1+Antibody/pm38982067-386-9-13
Average 93 stars, based on 2 article reviews
antibody to upf1 - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "UPF1 plays critical roles in early B cell development."
Article Title: UPF1 plays critical roles in early B cell development.
Journal: Nature communications
doi: 10.1038/s41467-024-50032-6
Figure Legend Snippet: Fig. 4 | UPF1 is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at
Techniques Used: RNA Sequencing, Derivative Assay, Negative Control
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Genotyping for Upf1-Flox forward GAATTGGTGGCATGGGTCCCATATCCCAG Genotyping for Upf1-Flox reverse GACTCTTCCTGGCCTTGAGCCTGGATG VHJ558 forward CGAGCTCTCCARCACAGCCTWCATGCARCTCARC VH7183 forward CGGTACCAAGAASAMCCTGTWCCTGCAAATGASC DH forward TTCAAAGCACAATGCCTGGCT JH3 reverse GTCTAGATTCTCACAAGAGTCCGATAGACCCTGG Cμ forward TGGCCATGGGCTGCCTAGCCCGGGACTT Cμ reverse GCCTGACTGAGCTCACACAAGGAGGA Vk forward GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC Jk reverse CTCATTCCTGTTGAAGCTCTTGACAATGGG Supplementary Table 1 List of primers used for PCR Supplementary Table 3 List of primers used for RT-qPCR Forward Reverse 18S rRNA CGGACAGGATTGACAGATTG CAAATCGCTCCACCAAGTAA mouse Upf1 GCTGAACTTCGAGGAAGATG CTTCCTTGCATTTTGCCCTC mouse Gas5 CAGGTATTAATGGGTCACCTC CTTCTATTTGAGCCTCCATCC mouse Mx1 GATCCGACTTCACTTCCAGATGG CATCTCAGTGGTAGTCAACCC mouse Mx2 TTCACCAGGCTCCGAAAAGAG ACAAACCCTGGCAATTCTCG Antibody Cat# Company Clone Application Dilution Magnetic Resonance Imaging:Article Title: Article Snippet: .. 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Article Snippet: Membranes were incubated with indicated primary antibodies and HRP-coupled secondary antibodies (NA9340; GE Healthcare). .. The following primary antibodies were used for immunoblot analysis: Article Title: Analysis and generation of B cell specific Upf1 deficient mice Enrichment score plot of down-regulated genes in Upf1-deficient early LPre-B cells Loss of IFNαR1 signaling does not impact B cell development. Genetic pre-arrangement of Igh locus rescued early to late LPre B cell differentiation in Upf1-cKO mice Genetic pre-arrangement of Igh locus did not rescue the splenic B cell population in Upf1-cKO mice Transcriptome analysis of Upf1-cKO/IghB1-8hi early LPre- and sPre-B cells Identifying UPF1-target RNAs Article Snippet: .. Genotyping for Upf1-Flox forward GAATTGGTGGCATGGGTCCCATATCCCAG Genotyping for Upf1-Flox reverse GACTCTTCCTGGCCTTGAGCCTGGATG VHJ558 forward CGAGCTCTCCARCACAGCCTWCATGCARCTCARC VH7183 forward CGGTACCAAGAASAMCCTGTWCCTGCAAATGASC DH forward TTCAAAGCACAATGCCTGGCT JH3 reverse GTCTAGATTCTCACAAGAGTCCGATAGACCCTGG Cμ forward TGGCCATGGGCTGCCTAGCCCGGGACTT Cμ reverse GCCTGACTGAGCTCACACAAGGAGGA Vk forward GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC Jk reverse CTCATTCCTGTTGAAGCTCTTGACAATGGG Supplementary Table 1 List of primers used for PCR Supplementary Table 3 List of primers used for RT-qPCR Forward Reverse 18S rRNA CGGACAGGATTGACAGATTG CAAATCGCTCCACCAAGTAA mouse Upf1 GCTGAACTTCGAGGAAGATG CTTCCTTGCATTTTGCCCTC mouse Gas5 CAGGTATTAATGGGTCACCTC CTTCTATTTGAGCCTCCATCC mouse Mx1 GATCCGACTTCACTTCCAGATGG CATCTCAGTGGTAGTCAACCC mouse Mx2 TTCACCAGGCTCCGAAAAGAG ACAAACCCTGGCAATTCTCG Antibody Cat# Company Clone Application Dilution Polymerase Chain Reaction:Article Title: Analysis and generation of B cell specific Upf1 deficient mice Enrichment score plot of down-regulated genes in Upf1-deficient early LPre-B cells Loss of IFNαR1 signaling does not impact B cell development. Genetic pre-arrangement of Igh locus rescued early to late LPre B cell differentiation in Upf1-cKO mice Genetic pre-arrangement of Igh locus did not rescue the splenic B cell population in Upf1-cKO mice Transcriptome analysis of Upf1-cKO/IghB1-8hi early LPre- and sPre-B cells Identifying UPF1-target RNAs Article Snippet: .. Genotyping for Upf1-Flox forward GAATTGGTGGCATGGGTCCCATATCCCAG Genotyping for Upf1-Flox reverse GACTCTTCCTGGCCTTGAGCCTGGATG VHJ558 forward CGAGCTCTCCARCACAGCCTWCATGCARCTCARC VH7183 forward CGGTACCAAGAASAMCCTGTWCCTGCAAATGASC DH forward TTCAAAGCACAATGCCTGGCT JH3 reverse GTCTAGATTCTCACAAGAGTCCGATAGACCCTGG Cμ forward TGGCCATGGGCTGCCTAGCCCGGGACTT Cμ reverse GCCTGACTGAGCTCACACAAGGAGGA Vk forward GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC Jk reverse CTCATTCCTGTTGAAGCTCTTGACAATGGG Supplementary Table 1 List of primers used for PCR Supplementary Table 3 List of primers used for RT-qPCR Forward Reverse 18S rRNA CGGACAGGATTGACAGATTG CAAATCGCTCCACCAAGTAA mouse Upf1 GCTGAACTTCGAGGAAGATG CTTCCTTGCATTTTGCCCTC mouse Gas5 CAGGTATTAATGGGTCACCTC CTTCTATTTGAGCCTCCATCC mouse Mx1 GATCCGACTTCACTTCCAGATGG CATCTCAGTGGTAGTCAACCC mouse Mx2 TTCACCAGGCTCCGAAAAGAG ACAAACCCTGGCAATTCTCG Antibody Cat# Company Clone Application Dilution Quantitative RT-PCR:Article Title: Analysis and generation of B cell specific Upf1 deficient mice Enrichment score plot of down-regulated genes in Upf1-deficient early LPre-B cells Loss of IFNαR1 signaling does not impact B cell development. Genetic pre-arrangement of Igh locus rescued early to late LPre B cell differentiation in Upf1-cKO mice Genetic pre-arrangement of Igh locus did not rescue the splenic B cell population in Upf1-cKO mice Transcriptome analysis of Upf1-cKO/IghB1-8hi early LPre- and sPre-B cells Identifying UPF1-target RNAs Article Snippet: .. 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