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gene exp gadd45a hs00169255 m1  (Thermo Fisher)


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    Thermo Fisher gene exp gadd45a hs00169255 m1
    Gene Exp Gadd45a Hs00169255 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Human+PCR+Microarray/Gene+Exp%2E+GADD45A%2C+Hs00169255_m1/pmc11106736-230-231-39
    Average 99 stars, based on 1 article reviews
    gene exp gadd45a hs00169255 m1 - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: Real Time PCR RNA extraction was performed at 24 and 48 hours after transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP ( Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1 α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000-WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: Real Time PCR RNA extraction was performed at 24 and 48 hours after transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP ( Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1 α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000-WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Mis-splicing drives loss of function of p53E224D point mutation.
    Article Snippet: DNase incubation was carried out for 15 minutes and each sample was quantified using a NanoDrop (Thermo Fisher). cDNA was generated from 1μg RNA using iScript cDNA Synthesis Kit (BioRad). .. Relative mRNA levels were determined using qPCR reactions performed on the QuantStudioTM 6 Flex Real-Time PCR System with TaqManTM Fast Advanced Master Mix (Thermo Fisher Scientific) and taqman probes designed to Cdkn1a (Mm04205640_g1, Hs00355782_m1), Mdm2 (Mm01233136_m1, Hs00540450_s1), p53 1-2 (Mm01731287_m1), p53 5-6 (Mm01731290_g1), p53 8-9 (Mm00441964_g1), Bbc3 (Mm00519268_m1), Gadd45a (Hs00169255_m1), 14-3-3σ (Hs00968567_s1). .. Levels of mRNAs were normalized to the level of Actb (Mm02619580_g1) or GAPDH (Hs02786624_ g1) mRNA.

    Article Title:
    Article Snippet: .. Quantitative reverse transcription real-time PCR was performed with the following Taqman primer/probe sets from Life Technologies (Grand Island, NY): Human: CHOP/DDIT3 (Hs01090850_m1), 18s (Hs99999901_s1), GADD34 (Hs00169585_m1), ATF3 (Hs00910173_m1), ATF4 (Hs00909569_g1), BiP/GRP78 (Hs99999174_m1), DR5 (Hs00366278_m1), TRB3 (Hs00221754_m1), XBP1s (spliced) (Hs03929085_g1), BIM (Hs00708019_s1), NOXA (Hs00560402_m1), PUMA (Hs00248075_m1), GADD45α (Hs00169255_m1), CEBPβ (Hs00270923_s1), and IRE1α (Hs00176385_m1); Mouse: Noxa (Mm00451763_m1), Puma (Mm00519268_m1), Dr5 (Mm00457866_m1), Trb3 (Mm00454879_m1), and Gadd45β (Mm00435123_m1). ..

    Gene Expression:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: Real Time PCR RNA extraction was performed at 24 and 48 hours after transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP ( Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1 α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000-WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Pyrolysis Gas Chromatography:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: Real Time PCR RNA extraction was performed at 24 and 48 hours after transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP ( Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1 α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000-WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: RNA extraction was performed at 24 and 48 hafter transfection by a TaqMan Gene Expression Cells-to-CTTM Kit (AM128, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. PCR was performed on a CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA) with 4 μL of cDNA, 10 μL of TaqMan Gene Expression Master Mix (4369016, Applied Biosystems, Waltham, MA, USA), 1 μl of c-Myc (Hs00153408_m1, Applied Biosystems, Waltham, MA, USA), PGC-1α (Hs00173304_m1, Applied Biosystems, Waltham, MA, USA), AMPK (Hs00272166_m1, Applied Biosystems, Waltham, MA, USA), CHOP (Hs00358796_g1, Applied Biosystems, Waltham, MA, USA), GADD45 alpha (Hs00169255_m1, Applied Biosystems, Waltham, MA, USA) and GAPDH (Hs99999905_m1, Applied Biosystems, Waltham, MA, USA) and 5 μL of nuclease-free water (B-003000WB-100, DharmaconTM, Cambridge, UK), and analyzed by the relative quantitative method. ..

    other:

    Article Title: Compounds and methods for treating cancer
    Article Snippet: Cells were stained with Propidium Iodide (BD Pharmingen).

    TaqMan Assay:

    Article Title: KT-253, a Novel MDM2 Degrader and p53 Stabilizer, Has Superior Potency and Efficacy than MDM2 Small-Molecule Inhibitors
    Article Snippet: .. Gene Symbol Gene Name TaqMan Assay ID MDM2 Mouse Double Minute 2 Hs99999008_m1 GDF15 Growth Differentiation Factor 15 Hs00171132_m1 CDKN1A Cyclin Dependent Kinase Inhibitor 1A Hs00355782_m1 GADD45A Growth Arrest and DNA-Damage-Inducible 45 Alpha Hs00169255_m1 TNFRSF10B Tumor Necrosis Factor Receptor Superfamily, Member 10B Hs00366278_m1 FAS Tumor Necrosis Factor Receptor Superfamily, Member 6 Hs00531110_m1 BBC3 BCL2 Binding Component 3 Hs00248075_m1 IPO8 Importin 8 Hs00914057_m1 ..

    Binding Assay:

    Article Title: KT-253, a Novel MDM2 Degrader and p53 Stabilizer, Has Superior Potency and Efficacy than MDM2 Small-Molecule Inhibitors
    Article Snippet: .. Gene Symbol Gene Name TaqMan Assay ID MDM2 Mouse Double Minute 2 Hs99999008_m1 GDF15 Growth Differentiation Factor 15 Hs00171132_m1 CDKN1A Cyclin Dependent Kinase Inhibitor 1A Hs00355782_m1 GADD45A Growth Arrest and DNA-Damage-Inducible 45 Alpha Hs00169255_m1 TNFRSF10B Tumor Necrosis Factor Receptor Superfamily, Member 10B Hs00366278_m1 FAS Tumor Necrosis Factor Receptor Superfamily, Member 6 Hs00531110_m1 BBC3 BCL2 Binding Component 3 Hs00248075_m1 IPO8 Importin 8 Hs00914057_m1 ..

    Reverse Transcription:

    Article Title:
    Article Snippet: .. Quantitative reverse transcription real-time PCR was performed with the following Taqman primer/probe sets from Life Technologies (Grand Island, NY): Human: CHOP/DDIT3 (Hs01090850_m1), 18s (Hs99999901_s1), GADD34 (Hs00169585_m1), ATF3 (Hs00910173_m1), ATF4 (Hs00909569_g1), BiP/GRP78 (Hs99999174_m1), DR5 (Hs00366278_m1), TRB3 (Hs00221754_m1), XBP1s (spliced) (Hs03929085_g1), BIM (Hs00708019_s1), NOXA (Hs00560402_m1), PUMA (Hs00248075_m1), GADD45α (Hs00169255_m1), CEBPβ (Hs00270923_s1), and IRE1α (Hs00176385_m1); Mouse: Noxa (Mm00451763_m1), Puma (Mm00519268_m1), Dr5 (Mm00457866_m1), Trb3 (Mm00454879_m1), and Gadd45β (Mm00435123_m1). ..



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    Effects of melatonin and radiation on breast cancer miRNA expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Cell Cycle Rt 2 Pcr Microarrays, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cytokine/chemokine rt2 profilertm pcr microarray  (Qiagen)
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    Qiagen human cytokine/chemokine rt2 profilertm pcr microarray
    Effects of melatonin and radiation on breast cancer miRNA expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Cytokine/Chemokine Rt2 Profilertm Pcr Microarray, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Human+PCR+Microarray/human+cytokine+chemokine+rt2+profilertm+pcr+microarray/pmc07815439-210-2-8
    Average 90 stars, based on 1 article reviews
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    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control