gene microarray human breast cancer rt 2 profiler tm pcr array (Qiagen)

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Qiagen gene microarray human breast cancer rt 2 profiler tm pcr array

Effects of melatonin and radiation on breast cancer miRNA expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

Gene Microarray Human Breast Cancer Rt 2 Profiler Tm Pcr Array, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Human+PCR+Microarray/human+breast+cancer+pcr+array/pmc09138876-68-21-40
Average 90 stars, based on 1 article reviews

gene microarray human breast cancer rt 2 profiler tm pcr array - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells"

Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

Journal: Biomedicines

doi: 10.3390/biomedicines10051088

Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

Figure Legend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

Techniques Used: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control

Polymerase Chain Reaction:

Article Title: Mechanism of Fibronectin-Damp-TLR-Mediated Regulation of Fibro-Inflammatory Phenotype and Tumor Progression
Article Snippet: First Strand kit (Qiagen) was used to convert 0.5 ng of RNA into cDNA as per the manufacturer’s recommended procedure. .. The cDNA was added to an RT” Profiler Human Inflammatory Response and Autoimmunity PCR Array (Qiagen; PAHS-077Z; A1F, HDF, and HKF) or Human Breast Cancer PCR Array (Qiagen; PAHS- 131ZA; MDA-MB-468) and real-time PCR analysis was performed in a thermocycler 30 .. (Bio-Rad) using a SYBRGreen (Qiagen) probe.

Article Title: Identification of antimicrobial peptides from the Ambystoma mexicanum displaying antibacterial and antitumor activity.
Article Snippet: To create cDNA the iScript cDNA Synthesis Kit (BioRad) was used and followed according to manufacturer’s instructions. .. For gene expression analysis, the Human Breast Cancer RT2 Profiler PCR array was used (Qiagen) according to manufacturer’s instructions. ..

Article Title: Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract
Article Snippet: .. In breast cancer and melanoma cell lines, effects of AgNPs and ASP on gene expressions were assessed by real time online RT-PCR (LightCycler 480 II) by using RT2 Profiler PCR Array Human Breast Cancer and Human Melanoma PCR Array, Qiagen Kits. .. Total RNA extraction and cDNA were performed according to the kit manual, by using RNeasy Mini Kit (Qiagen) and RT2 First Strand Kit http://dx.doi.org/10.29228/jrp.413 J Res Pharm 2023; 27(3): 1242-1251 cDNA Synthesis kit (Qiagen), respectively [33].

Article Title: Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro
Article Snippet: .. We used an RT2 ProfilerTM PCR Array Human Breast Cancer kit (Qiagen Cat# PAHS-131ZR) to analyze modulation in the expression of molecules involved in breast oncogenesis and cancer progression at the RNA level. .. Simultaneously, we used RT2 ProfilerTM PCR Array Human Epithelial to Mesenchymal Transition (Qiagen Cat# PAHS-090ZA) to analyze the modulation in expression of genes responsible for EMT at the RNA level. shows the expression of several molecules with tumor suppressor or oncogenic properties in MDA231 cells after treatment with the cellular component or secretome of preconditioned CVMSCs.

Article Title: Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro.
Article Snippet: A QuantiTect Reverse Transcription Kit (cat# 205313, Qiagen, MD, USA) was used to transcribe RNA into the single stranded cDNA. .. Realtime PCR reaction was performed to detect the expression of 84 genes related to Human Breast Cancer using an RT2 Profiler Kit (cat# PAHS-131ZA, Qiagen, Hilden, Germany) and the RT2 Profiler PCR Array Human Epithelial to Mesenchymal Transition (Qiagen Cat# PAHS-090ZA) to analyze modulation in the expression of genes responsible for EMT on the CFX96 real-time PCR detection system (Bio-Rad, CA, USA). .. Data analysis was performed using the CFX manager software (Bio-Rad, CA, USA).

Article Title: Modulation of wheatgrass ( Triticum aestivum Linn ) toxicity against breast cancer cell lines by simulated microgravity
Article Snippet: A total of 0.5 μg of RNA was used to synthesize cDNA for each sample using the RT2 First Strand Kit® (Qiagen GmbH), whereby genomic DNA was eliminated and then reverse-transcribed to cDNA. .. Real time-qPCR: The Breast cancer profiler PCR array (cat. No. PAHS-131ZR, Qiagen GmbH) and cell death profiler PCR array (cat. No. PAHS-212ZR, Qiagen GmbH) kits were used( ). ..

Multiple Displacement Amplification:

Real-time Polymerase Chain Reaction:

Gene Expression:

Reverse Transcription Polymerase Chain Reaction:

other:

Article Title: Personalized Versus Precision Nanomedicine for Treatment of Ovarian Cancer.
Article Snippet: For the initial analysis, we selected three commercially available cancer profiling qRT-PCR kits: Human Apoptosis[1] and Breast Cancer[2] RT2 ProfilerTM PCR Arrays and qBiomarker Copy Number PCR Array Human Ovarian Cancer[3] available from Qiagen (Valencia, CA).

Article Title: Interleukin-30 subverts prostate cancer-endothelium crosstalk by fostering angiogenesis and activating immunoregulatory and oncogenic signaling pathways.
Article Snippet: Real-time RT-PCR and PCR array were performed, as described in the Supplementary Methods, using the RT2 Profiler Human Angiogenesis PCR Array (#PAHS024ZR), RT2 Profiler Human Cancer Inflammation & Immunity Crosstalk PCR Array (#PAHS-181Z) and the RT2 ProfilerTM Human Prostate Cancer PCR Array (#PAHS-135Z) (all from Qiagen, Hilden, Germany).

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