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gene exp mmp2 hs00234422 m1  (Thermo Fisher)


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    Thermo Fisher gene exp mmp2 hs00234422 m1
    Molecular and functional analysis of BMMSCs. (A): Percentages of positive cells for subsequent surface markers are given in table. (B): Graphical overview of comparative genomic hybridization microarray results. Expression levels of mRNA for neurotrophic (C) , proangiogenic (D) , and tissue remodeling (E) factors in patients' BMMSCs. qRT‐PCR analysis revealed expression of BDNF, NGF, HGF, PDGF‐A, Flt‐1, VEGF‐A, FGF‐1, FGF‐2, ANGPT‐1, and <t>MMP‐2.</t> Slight expression of GDNF, CNTF, and NGF was observed. No expression of MMP‐9 was detected. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. (F): Western blot detection of VEGF in patients' BMMSCs lysates. Both forms of VEGF were identified: dimer (42 kDa) and monomer (21 kDa). The intermediary band is an effect of nonspecific reaction of antibody, observed also in manufacturer's specification. Standardized protein content in samples from different patients was confirmed by GAPDH detection. (G, H): qRT‐PCR analysis of IDO1 and TSG‐6 mRNA expression levels in patients' BMMSCs stimulated with proinflammatory cytokines. (G): Pretreatment of BMMSCs with INF‐γ caused manifestation of IDO1 expression. (H): Moreover, stimulation of BMMSCs with either TNF‐α or Il‐1β revealed elevated expression of TSG‐6. Pronounced effect was observed for Il‐1β. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. As a control in experiments, BMMSCs in stimulation medium only (2% FBS) were utilized. Abbreviations: ANGPT‐1, angiopoietin 1; BDNF, brain‐derived neurotrophic factor; BMMSC, bone marrow mesenchymal stem cell; CNTF, ciliary neurotrophic factor; FBS, fetal bovine serum; FGF, fibroblast growth factor; Flt‐1, vascular endothelial growth factor receptor 1; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; GDNF, glial‐derived neurotrophic factor; HGF, hepatocyte growth factor; IDO1, indoleamine 2,3‐dioxygenase; Il‐1β, interleukin 1 β; INF‐γ, interferon γ; MMP, metalloproteinase; NGF, nerve growth factor; PDGF, platelet‐derived growth factor; qRT‐PCR, real‐time quantitative reverse transcription polymerase chain reaction; TNF‐α, tumor necrosis factor α; TSG‐6, tumor necrosis factor‐inducible gene 6; VEGF, vascular endothelial growth factor.
    Gene Exp Mmp2 Hs00234422 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Human+PCR+Microarray/Gene+Exp%2E+MMP2%2C+Hs00234422_m1/pmc05746144-154-103-15
    Average 99 stars, based on 1 article reviews
    gene exp mmp2 hs00234422 m1 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Multiple Autologous Bone Marrow‐Derived CD271 + Mesenchymal Stem Cell Transplantation Overcomes Drug‐Resistant Epilepsy in Children"

    Article Title: Multiple Autologous Bone Marrow‐Derived CD271 + Mesenchymal Stem Cell Transplantation Overcomes Drug‐Resistant Epilepsy in Children

    Journal: Stem Cells Translational Medicine

    doi: 10.1002/sctm.17-0041

    Molecular and functional analysis of BMMSCs. (A): Percentages of positive cells for subsequent surface markers are given in table. (B): Graphical overview of comparative genomic hybridization microarray results. Expression levels of mRNA for neurotrophic (C) , proangiogenic (D) , and tissue remodeling (E) factors in patients' BMMSCs. qRT‐PCR analysis revealed expression of BDNF, NGF, HGF, PDGF‐A, Flt‐1, VEGF‐A, FGF‐1, FGF‐2, ANGPT‐1, and MMP‐2. Slight expression of GDNF, CNTF, and NGF was observed. No expression of MMP‐9 was detected. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. (F): Western blot detection of VEGF in patients' BMMSCs lysates. Both forms of VEGF were identified: dimer (42 kDa) and monomer (21 kDa). The intermediary band is an effect of nonspecific reaction of antibody, observed also in manufacturer's specification. Standardized protein content in samples from different patients was confirmed by GAPDH detection. (G, H): qRT‐PCR analysis of IDO1 and TSG‐6 mRNA expression levels in patients' BMMSCs stimulated with proinflammatory cytokines. (G): Pretreatment of BMMSCs with INF‐γ caused manifestation of IDO1 expression. (H): Moreover, stimulation of BMMSCs with either TNF‐α or Il‐1β revealed elevated expression of TSG‐6. Pronounced effect was observed for Il‐1β. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. As a control in experiments, BMMSCs in stimulation medium only (2% FBS) were utilized. Abbreviations: ANGPT‐1, angiopoietin 1; BDNF, brain‐derived neurotrophic factor; BMMSC, bone marrow mesenchymal stem cell; CNTF, ciliary neurotrophic factor; FBS, fetal bovine serum; FGF, fibroblast growth factor; Flt‐1, vascular endothelial growth factor receptor 1; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; GDNF, glial‐derived neurotrophic factor; HGF, hepatocyte growth factor; IDO1, indoleamine 2,3‐dioxygenase; Il‐1β, interleukin 1 β; INF‐γ, interferon γ; MMP, metalloproteinase; NGF, nerve growth factor; PDGF, platelet‐derived growth factor; qRT‐PCR, real‐time quantitative reverse transcription polymerase chain reaction; TNF‐α, tumor necrosis factor α; TSG‐6, tumor necrosis factor‐inducible gene 6; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Molecular and functional analysis of BMMSCs. (A): Percentages of positive cells for subsequent surface markers are given in table. (B): Graphical overview of comparative genomic hybridization microarray results. Expression levels of mRNA for neurotrophic (C) , proangiogenic (D) , and tissue remodeling (E) factors in patients' BMMSCs. qRT‐PCR analysis revealed expression of BDNF, NGF, HGF, PDGF‐A, Flt‐1, VEGF‐A, FGF‐1, FGF‐2, ANGPT‐1, and MMP‐2. Slight expression of GDNF, CNTF, and NGF was observed. No expression of MMP‐9 was detected. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. (F): Western blot detection of VEGF in patients' BMMSCs lysates. Both forms of VEGF were identified: dimer (42 kDa) and monomer (21 kDa). The intermediary band is an effect of nonspecific reaction of antibody, observed also in manufacturer's specification. Standardized protein content in samples from different patients was confirmed by GAPDH detection. (G, H): qRT‐PCR analysis of IDO1 and TSG‐6 mRNA expression levels in patients' BMMSCs stimulated with proinflammatory cytokines. (G): Pretreatment of BMMSCs with INF‐γ caused manifestation of IDO1 expression. (H): Moreover, stimulation of BMMSCs with either TNF‐α or Il‐1β revealed elevated expression of TSG‐6. Pronounced effect was observed for Il‐1β. The results are mean values ± SD (from triplicates) of mRNA expression relative to expression of housekeeping gene—GAPDH. As a control in experiments, BMMSCs in stimulation medium only (2% FBS) were utilized. Abbreviations: ANGPT‐1, angiopoietin 1; BDNF, brain‐derived neurotrophic factor; BMMSC, bone marrow mesenchymal stem cell; CNTF, ciliary neurotrophic factor; FBS, fetal bovine serum; FGF, fibroblast growth factor; Flt‐1, vascular endothelial growth factor receptor 1; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; GDNF, glial‐derived neurotrophic factor; HGF, hepatocyte growth factor; IDO1, indoleamine 2,3‐dioxygenase; Il‐1β, interleukin 1 β; INF‐γ, interferon γ; MMP, metalloproteinase; NGF, nerve growth factor; PDGF, platelet‐derived growth factor; qRT‐PCR, real‐time quantitative reverse transcription polymerase chain reaction; TNF‐α, tumor necrosis factor α; TSG‐6, tumor necrosis factor‐inducible gene 6; VEGF, vascular endothelial growth factor.

    Techniques Used: Functional Assay, Hybridization, Microarray, Expressing, Quantitative RT-PCR, Western Blot, Control, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction

    Related Articles

    Expressing:

    Article Title: Inhibitory effect of Porphyromonas gingivalis ‐derived phosphoethanolamine dihydroceramide on acid ceramidase expression in oral squamous cells
    Article Snippet: The total RNA was isolated using PureLinkTM RNA Mini Kit (Life Technologies) and subjected to reverse transcription with the Verso cDNA Synthesis Kit (Thermo Fisher Scientific) in the presence of random primers and oligo-dT following the manufacturer's recommendations. .. The expression of human ASAH1 (Hs00602774_m1), ASAH2 (Hs01015655_m1), ACER1 (Hs00370322_m1), ACER2 (Hs04996319_g1), ACER3 (Hs00924388_m1), S1PR1 (Hs00173499_m1), S1PR3 (Hs00245464_S1), IL6 (Hs00985639_m1), NF-κB (Hs01042010_m1), MMP2(Hs00234422_m1), and ADAM17 (Hs01041915_m1) genes were measured using TaqManTM Fast Advanced PCR Master Mix (Applied Biosystems, Life Technologies). .. Data were analyzed using the 2-ΔΔCt method normalized to GAPDH (Hs02786624_g1).

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21.
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [41] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [44], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [ ] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [ ], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    Article Title: TRPV2 Promotes Cell Migration and Invasion in Gastric Cancer via the Transforming Growth Factor-β Signaling Pathway.
    Article Snippet: Background.. Transient receptor potential vanilloid 2 (TRPV2) is a highly Ca-permeable ion channel that is involved in a number of cellular processes.. It is expressed in various human cancers; however, the role of TRPV2 in gastric cancer (GC) remains poorly understood.

    Polymerase Chain Reaction:

    Article Title: Inhibitory effect of Porphyromonas gingivalis ‐derived phosphoethanolamine dihydroceramide on acid ceramidase expression in oral squamous cells
    Article Snippet: The total RNA was isolated using PureLinkTM RNA Mini Kit (Life Technologies) and subjected to reverse transcription with the Verso cDNA Synthesis Kit (Thermo Fisher Scientific) in the presence of random primers and oligo-dT following the manufacturer's recommendations. .. The expression of human ASAH1 (Hs00602774_m1), ASAH2 (Hs01015655_m1), ACER1 (Hs00370322_m1), ACER2 (Hs04996319_g1), ACER3 (Hs00924388_m1), S1PR1 (Hs00173499_m1), S1PR3 (Hs00245464_S1), IL6 (Hs00985639_m1), NF-κB (Hs01042010_m1), MMP2(Hs00234422_m1), and ADAM17 (Hs01041915_m1) genes were measured using TaqManTM Fast Advanced PCR Master Mix (Applied Biosystems, Life Technologies). .. Data were analyzed using the 2-ΔΔCt method normalized to GAPDH (Hs02786624_g1).

    Quantitative RT-PCR:

    Article Title: Prognostic Significance of Integrin Subunit Alpha 2 (ITGA2) and Role of Mechanical Cues in Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma (PDAC).
    Article Snippet: The MS proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE19 partner repository with the dataset identifier PXD010112. .. RT-qPCR was used to evaluate gene expression of ITGA2, as described above for the PDAC specimens of the first cohort, as well as to evaluate the gene expression of two genes which have been linked to PDAC invasion and metastasis [32], matrix metalloproteinase-2 (MMP2) and chemokine (C-X-C motif) receptor 4 (CXCR4), using the specific TaqMan® gene expression assays (Hs00234422_m1, and Hs00976734_m1). ..

    Article Title: Prognostic Significance of Integrin Subunit Alpha 2 (ITGA2) and Role of Mechanical Cues in Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma (PDAC)
    Article Snippet: The MS proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE19 partner repository with the dataset identifier PXD010112. .. RT-qPCR was used to evaluate gene expression of ITGA2, as described above for the PDAC specimens of the first cohort, as well as to evaluate the gene expression of two genes which have been linked to PDAC invasion and metastasis [ ], matrix metalloproteinase-2 (MMP2) and chemokine (C-X-C motif) receptor 4 (CXCR4), using the specific TaqMan ® gene expression assays (Hs00234422_m1, and Hs00976734_m1). ..

    Gene Expression:

    Article Title: Prognostic Significance of Integrin Subunit Alpha 2 (ITGA2) and Role of Mechanical Cues in Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma (PDAC).
    Article Snippet: The MS proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE19 partner repository with the dataset identifier PXD010112. .. RT-qPCR was used to evaluate gene expression of ITGA2, as described above for the PDAC specimens of the first cohort, as well as to evaluate the gene expression of two genes which have been linked to PDAC invasion and metastasis [32], matrix metalloproteinase-2 (MMP2) and chemokine (C-X-C motif) receptor 4 (CXCR4), using the specific TaqMan® gene expression assays (Hs00234422_m1, and Hs00976734_m1). ..

    Article Title: Prognostic Significance of Integrin Subunit Alpha 2 (ITGA2) and Role of Mechanical Cues in Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma (PDAC)
    Article Snippet: The MS proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE19 partner repository with the dataset identifier PXD010112. .. RT-qPCR was used to evaluate gene expression of ITGA2, as described above for the PDAC specimens of the first cohort, as well as to evaluate the gene expression of two genes which have been linked to PDAC invasion and metastasis [ ], matrix metalloproteinase-2 (MMP2) and chemokine (C-X-C motif) receptor 4 (CXCR4), using the specific TaqMan ® gene expression assays (Hs00234422_m1, and Hs00976734_m1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21.
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [41] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [44], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [ ] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [ ], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    Biomarker Discovery:

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21.
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [41] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [44], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21
    Article Snippet: The reverse transcription of messenger RNA and micro-RNA was performed as previously described [ ] using superscript II (Invitrogen, life Technologies) and TaqMan micro-RNA reverse transcription kits (Applied biosystems, Thermo Fisher Scientific, Oxford, UK), respectively. .. Quantitative real time PCR was performed using TaqMan predesigned expression assay for E-cadherin (Hs01023895_m1) selected as a biomarker of EMT transition, BCL-2 (Hs04986394_s1) a predicted mRNA target of miR-21 obtained using TargetScan version 7.0 [ ], GAPDH (Hs99999905_m1), MMP2 (Hs00234422_m1), VEGF-A (Hs00900054_m1), U6 (TM&RT:001973), and hsa-miR-21-5p (TM&RT:000397). .. Fast PCR master mix (TaqMan, applied biosystem, life technologies) was used according to the manufacturer’s protocol.

    other:

    Article Title: Impact of AMPK on cervical carcinoma progression and metastasis
    Article Snippet: CDH1 , Hs01023894_m1 , MMP-2 , Hs00234422_m1.



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    Qiagen human cytokine/chemokine rt2 profilertm pcr microarray
    Effects of melatonin and radiation on breast cancer miRNA expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Cytokine/Chemokine Rt2 Profilertm Pcr Microarray, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control