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sirna targeting kras proto oncogene gtpase kras  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology sirna targeting kras proto oncogene gtpase kras
    BRD4 knockdown attenuates the proliferation and promotes the apoptosis of U251 cells. (A) A Cell counting kit-8 assay was performed to detect the proliferation of U251 cells transduced with <t>BRD4-shRNA</t> or Scr-shRNA. Each experiment was performed in triplicate. (B) An EdU assay was performed to determine cell proliferation rates following BRD4 knockdown. Nuclei were counterstained blue with DAPI. Red indicates the cells undergoing proliferation. Scale bar, 20 µ m. (C) Quantification of the EdU assay results demonstrated that U251 cell proliferation was reduced following BRD4 knockdown. (D) Representative plots and quantification of flow cytometry analysis of apoptosis following BRD4 knockdown in U251 cells. Data are presented as the mean ± standard deviation of three independent experiments. ** P<0.01. BRD4, bromodomain containing 4; sh, short hairpin; Scr, scrambled control; OD, optical density.
    Sirna Targeting Kras Proto Oncogene Gtpase Kras, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Human+PCR+Microarray/K-Ras+siRNA/pmc05873870-90-14-34
    Average 93 stars, based on 30 article reviews
    sirna targeting kras proto oncogene gtpase kras - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Genome-wide transcriptional analysis of BRD4-regulated genes and pathways in human glioma U251 cells"

    Article Title: Genome-wide transcriptional analysis of BRD4-regulated genes and pathways in human glioma U251 cells

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2018.4324

    BRD4 knockdown attenuates the proliferation and promotes the apoptosis of U251 cells. (A) A Cell counting kit-8 assay was performed to detect the proliferation of U251 cells transduced with BRD4-shRNA or Scr-shRNA. Each experiment was performed in triplicate. (B) An EdU assay was performed to determine cell proliferation rates following BRD4 knockdown. Nuclei were counterstained blue with DAPI. Red indicates the cells undergoing proliferation. Scale bar, 20 µ m. (C) Quantification of the EdU assay results demonstrated that U251 cell proliferation was reduced following BRD4 knockdown. (D) Representative plots and quantification of flow cytometry analysis of apoptosis following BRD4 knockdown in U251 cells. Data are presented as the mean ± standard deviation of three independent experiments. ** P<0.01. BRD4, bromodomain containing 4; sh, short hairpin; Scr, scrambled control; OD, optical density.
    Figure Legend Snippet: BRD4 knockdown attenuates the proliferation and promotes the apoptosis of U251 cells. (A) A Cell counting kit-8 assay was performed to detect the proliferation of U251 cells transduced with BRD4-shRNA or Scr-shRNA. Each experiment was performed in triplicate. (B) An EdU assay was performed to determine cell proliferation rates following BRD4 knockdown. Nuclei were counterstained blue with DAPI. Red indicates the cells undergoing proliferation. Scale bar, 20 µ m. (C) Quantification of the EdU assay results demonstrated that U251 cell proliferation was reduced following BRD4 knockdown. (D) Representative plots and quantification of flow cytometry analysis of apoptosis following BRD4 knockdown in U251 cells. Data are presented as the mean ± standard deviation of three independent experiments. ** P<0.01. BRD4, bromodomain containing 4; sh, short hairpin; Scr, scrambled control; OD, optical density.

    Techniques Used: Knockdown, Cell Counting, Transduction, shRNA, EdU Assay, Flow Cytometry, Standard Deviation, Control

    Ten key genes identified by global signal transduction network analysis.
    Figure Legend Snippet: Ten key genes identified by global signal transduction network analysis.

    Techniques Used: Transduction

    Experimental validation of microarray results. (A) Reverse transcription-quantitative polymerase chain reaction results for the mRNA expression levels of the ten key genes identified by global signal transduction network analysis. (B) Western blotting validation of the protein expression changes of key genes in the BRD4-shRNA and the Scr-shRNA groups. GAPDH was used as an internal control. (C) Representative photographs and quantification of KRAS immunostaining in normal brain tissue and glioma tissues of grades II, III and IV. Scale bar, 20 µ m. (D) Western blot analysis of KRAS levels in HA and U251 cells. (E) KRAS silencing following siRNA transfection in U251 cells was confirmed by western blotting (at 72 h post-transfection). (F) A cell counting kit-8 assay was performed to detect the proliferation rates of siKRAS and or siCon-transfected U251 cells. Each experiment was performed in triplicate. (G) The apoptosis rates of siKRAS and siCon-transfected U251 cells were determined by TUNEL staining (red). Nuclei were counterstained with DAPI (blue). Scar bar, 50 µ m. Experimental data are presented as the mean ± standard deviation of at least three experiments. * P<0.05 and ** P<0.01. BRD4, bromodomain containing 4; sh, short hairpin; Scr, scrambled control; KRAS, KRAS proto-oncogene GTPase; HA, human astrocytes; si, small interfering; Con, control; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labelling; OD, optical density.
    Figure Legend Snippet: Experimental validation of microarray results. (A) Reverse transcription-quantitative polymerase chain reaction results for the mRNA expression levels of the ten key genes identified by global signal transduction network analysis. (B) Western blotting validation of the protein expression changes of key genes in the BRD4-shRNA and the Scr-shRNA groups. GAPDH was used as an internal control. (C) Representative photographs and quantification of KRAS immunostaining in normal brain tissue and glioma tissues of grades II, III and IV. Scale bar, 20 µ m. (D) Western blot analysis of KRAS levels in HA and U251 cells. (E) KRAS silencing following siRNA transfection in U251 cells was confirmed by western blotting (at 72 h post-transfection). (F) A cell counting kit-8 assay was performed to detect the proliferation rates of siKRAS and or siCon-transfected U251 cells. Each experiment was performed in triplicate. (G) The apoptosis rates of siKRAS and siCon-transfected U251 cells were determined by TUNEL staining (red). Nuclei were counterstained with DAPI (blue). Scar bar, 50 µ m. Experimental data are presented as the mean ± standard deviation of at least three experiments. * P<0.05 and ** P<0.01. BRD4, bromodomain containing 4; sh, short hairpin; Scr, scrambled control; KRAS, KRAS proto-oncogene GTPase; HA, human astrocytes; si, small interfering; Con, control; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labelling; OD, optical density.

    Techniques Used: Biomarker Discovery, Microarray, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Transduction, Western Blot, shRNA, Control, Immunostaining, Transfection, Cell Counting, TUNEL Assay, Staining, Standard Deviation

    Related Articles

    shRNA:

    Article Title: High level of ANO1 promotes pancreatic cancer growth in concert with oncogenic KRAS.
    Article Snippet: Background Anoctamin-1 (ANO1) was identified as an unfavorable prognostic marker in pancreatic cancer.. However, the exact implication of ANO1 in pancreatic cancer is still poorly understood.. Here we investigated the effect of ANO1 in pancreatic cancer progression under the context of oncogenic KRAS, aiming at finding a new therapeutic target.

    Control:

    Article Title: Inhibiting Isoprenylation Suppresses FcεRI-Mediated Mast Cell Function and Allergic Inflammation.
    Article Snippet: OVA-HSA was obtained from Sigma-Aldrich. .. Alternaria alternata was obtained from Greer Laboratories (Lenoir, NC). siRNAs for N-Ras (sc-36005), K-Ras (sc-43876), and scramble control were obtained from Santa Cruz Biotechnology. siRNAs for pggt1b (GGT; SIO1376228, SI01376221, SI01376207, and SI01376207) and fnta (FT; SI01004360, SI01004353, SI01004339, SI01004346) were obtained from Qiagen (Redwood City, CA). .. IC fixation buffer and permeabilization buffer were purchased from Thermo Fisher.

    Negative Control:

    Article Title: Combination Therapy with KRAS and P38α siRNA Suppresses Colorectal Cancer Growth and Development in SW480 Cell Line.
    Article Snippet: .. Human KRAS siRNA (sc-35731) and human p38α siRNA (sc-29433), which contain three dissimilar nucleotide sequences (19–25 bp) in length for each siRNA, siRNA scrambled siRNA which have no homology with any human genes and called negative control (NC) (sc-37007), siRNA transfection medium (TM) (SC-36868), and siRNA transfection reagent (TR) (SC-29528), have been bought from Santa Cruz Biotechnology (CA). .. SYBR green master mix for real-time PCR was obtained from Ampliqon (catalog number: A323402; Ampliqon, Stenhuggervej, Denmark).

    Transfection:

    Article Title: Combination Therapy with KRAS and P38α siRNA Suppresses Colorectal Cancer Growth and Development in SW480 Cell Line.
    Article Snippet: .. Human KRAS siRNA (sc-35731) and human p38α siRNA (sc-29433), which contain three dissimilar nucleotide sequences (19–25 bp) in length for each siRNA, siRNA scrambled siRNA which have no homology with any human genes and called negative control (NC) (sc-37007), siRNA transfection medium (TM) (SC-36868), and siRNA transfection reagent (TR) (SC-29528), have been bought from Santa Cruz Biotechnology (CA). .. SYBR green master mix for real-time PCR was obtained from Ampliqon (catalog number: A323402; Ampliqon, Stenhuggervej, Denmark).



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    Image Search Results


    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control