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af1663 rrid ab 2233171  (R&D Systems)


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    R&D Systems af1663 rrid ab 2233171
    Af1663 Rrid Ab 2233171, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AF1663/Human%2FMouse%2FRat+HSP70%2FHSPA1A+Antibody/pmc12408481-131-39-44
    Average 93 stars, based on 20 article reviews
    af1663 rrid ab 2233171 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Evaluating endometrial response to human chorionic gonadotropin: alterations in epigenetic regulation and extracellular vesicle cargo of endometrial stromal cells
    Article Snippet: .. For EVs characterization immunoblot analysis was performed as described in ‘Immunoblot analysis’ using these antibodies: TSG101 (Abcam Cat No. ab125011, RRID: AB_10974262), Alix (Abcam Cat No. ab88388, RRID: AB_2042596), HSP70 (R & D Systems (Minneapolis, MN, USA) Cat No. AF1663, RRID: AB_2233171), Annexin V (R & D Systems Cat No. AF399, RRID: AB_2258445), CD9 (R & D Systems Cat No. MAB25292, RRID: AB_2910230), Cytochrome c (Novus (St Louis, MN, USA) Cat No. NB100-56503, RRID: AB_838084), GM130 (Novus Cat No. NBP2-53420, RRID: AB_2916095), CD81 (Novus Cat No. NB100-65805, RRID: AB_962702). .. The sizes and counts of isolated EVs were evaluated using a nanoparticle tracking analyzer, NanoSight LM10-HS (NanoSight, Salisbury, UK).

    Incubation:

    Article Title: CD20-bearing extracellular vesicles are associated with prognostic biomarkers of patients with AIDS-NHL.
    Article Snippet: Samples were then loaded into each well of a precast 4–12% Bis-Tris Mini Protein Gel (1.0 mm) (3.5 to 260 kDa) (Invitrogen, Carlsbad, CA, USA), run at 130 V, and then transferred to a Millipore Immobilin®-FL PVDF Membrane (0.45 μm) using the Mini-Protean Tetra System (Bio-Rad, Hercules, CA, USA) at 100 V for 1 h. Membranes were then immersed in 100% methanol for 30 s, rinsed once in double distilled water, and then rinsed in 1X Intercept® TBS Blocking Buffer (1X TBS) (LI-COR Biosciences, Lincoln, NE, USA) for 5 min and blocked in 1X TBS for 1 h at room temperature with gentle shaking. .. Membranes were then incubated with the following primary antibodies: mouse monoclonal anti-human CD9 (Catalog No. 602.29 cl.11; Developmental Studies Hybridoma Bank, Iowa City, IA, USA) at 1:50 multiplexed with rabbit polyclonal anti-human TSG101 (Catalog No. 28283-1-AP; Proteintech, Rosemont, IL, USA) at 1:2,000; mouse monoclonal anti-human CD81 (clone 1D6) (Catalog No. NB10065805; Novus Biologicals, Centennial, CO, USA) at 1:100 multiplexed with rabbit polyclonal anti-human HSP70/HSPA1A (Catalog No. AF1663; R&D Systems, Minneapolis, MN, USA) at 1:1000; and anti-human CD63 (Catalog No. H5C6; Developmental Studies Hybridoma Bank, Iowa City, IA, USA). ..

    Article Title: Changes to the Autophagy-Related Muscle Proteome Following Short-Term Treatment with Ectoine in the Duchenne Muscular Dystrophy Mouse Model mdx
    Article Snippet: .. The primary antibodies were diluted in PBS/BSA/HD and incubated for 3 h with the following concentrations of antibodies: 1.1 μg/mL rabbit monoclonal anti-ATG5 (D5F5U), 0.2 μg/mL rabbit monoclonal anti-ATG7 (D12B11), 0.9 μg/mL rabbit monoclonal anti-ATG16L1 (D6D5), 0.2 μg/mL rabbit polyclonal anti-PRKN, 1 μg/mL rabbit monoclonal anti-LC3 II (D11), 0.5 μg/mL rabbit monoclonal anti-SQSTM1 (D6M5X) (Cell Signaling Technologies), 10 μg/mL rabbit polyclonal anti-HSP70 (R&D Systems), 5 μg/mL rat anti-F4/80 (Abcam) and 2.5 μg/mL goat polyclonal anti-NCAM (R&D Systems). .. Sections were rinsed three times with PBS and incubated for 30 min with secondary antibodies: 0.25 μg/mL donkey anti-rabbit AlexaFluor 488 conjugated (Thermo Fisher Scientific) and 0.25 μg/mL donkey anti-goat CY3 conjugated (Jackson Laboratories, West Grove, PA, USA) diluted in PBS.

    Article Title: CD20-bearing extracellular vesicles are associated with prognostic biomarkers of patients with AIDS-NHL
    Article Snippet: Samples were then loaded into each well of a pre-cast 4–12% Bis-Tris Mini Protein Gel (1.0 mm) (3.5 to 260 kDa) (Invitrogen, Carlsbad, CA, USA), run at 130 V, and then transferred to a Millipore Immobilin ® -FL PVDF Membrane (0.45 μm) using the Mini-Protean Tetra System (Bio-Rad, Hercules, CA, USA) at 100 V for 1 h. Membranes were then immersed in 100% methanol for 30 s, rinsed once in double distilled water, and then rinsed in 1X Intercept ® TBS Blocking Buffer (1X TBS) (LI-COR Biosciences, Lincoln, NE, USA) for 5 min and blocked in 1X TBS for 1 h at room temperature with gentle shaking. .. Membranes were then incubated with the following primary antibodies: mouse monoclonal anti-human CD9 (Catalog No. 602.29 cl.11; Developmental Studies Hybridoma Bank, Iowa City, IA, USA) at 1:50 multiplexed with rabbit polyclonal anti-human TSG101 (Catalog No. 28283-1-AP; Proteintech, Rosemont, IL, USA) at 1:2,000; mouse monoclonal anti-human CD81 (clone 1D6) (Catalog No. NB10065805; Novus Biologicals, Centennial, CO, USA) at 1:100 multiplexed with rabbit polyclonal anti-human HSP70/HSPA1A (Catalog No. AF1663; R&D Systems, Minneapolis, MN, USA) at 1:1000; and anti-human CD63 (Catalog No. H5C6; Developmental Studies Hybridoma Bank, Iowa City, IA, USA). ..

    Recombinant:

    Article Title: Quantitative assay for heat shock proteins 70 (Hsp70) protein in body fluids
    Article Snippet: .. Suitable capture reagents for Hsp70, in particular polyclonal antibodies against recombinant human Hsp70, detectable labels/ligands, ligand-binding tag; calibrated immunoassay standards and controls of recombinant human Hsp70 as well as recommendations for microplates, buffers, diluents, substrates and/or solutions as well as instructions how to perform an Hsp70 ELISA are generally known; see, e.g., commercially available Hsp70 kits from Human/Mouse/Rat Total Hsp70/HspA1A DuoSet IC (R&D Systems, Minneapolis, Minn., USA), containing the basic components required for the development of sandwich ELISAs to measure Hsp70/HspA1A in cell lysates. ..

    Ligand Binding Assay:

    Article Title: Quantitative assay for heat shock proteins 70 (Hsp70) protein in body fluids
    Article Snippet: .. Suitable capture reagents for Hsp70, in particular polyclonal antibodies against recombinant human Hsp70, detectable labels/ligands, ligand-binding tag; calibrated immunoassay standards and controls of recombinant human Hsp70 as well as recommendations for microplates, buffers, diluents, substrates and/or solutions as well as instructions how to perform an Hsp70 ELISA are generally known; see, e.g., commercially available Hsp70 kits from Human/Mouse/Rat Total Hsp70/HspA1A DuoSet IC (R&D Systems, Minneapolis, Minn., USA), containing the basic components required for the development of sandwich ELISAs to measure Hsp70/HspA1A in cell lysates. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Quantitative assay for heat shock proteins 70 (Hsp70) protein in body fluids
    Article Snippet: .. Suitable capture reagents for Hsp70, in particular polyclonal antibodies against recombinant human Hsp70, detectable labels/ligands, ligand-binding tag; calibrated immunoassay standards and controls of recombinant human Hsp70 as well as recommendations for microplates, buffers, diluents, substrates and/or solutions as well as instructions how to perform an Hsp70 ELISA are generally known; see, e.g., commercially available Hsp70 kits from Human/Mouse/Rat Total Hsp70/HspA1A DuoSet IC (R&D Systems, Minneapolis, Minn., USA), containing the basic components required for the development of sandwich ELISAs to measure Hsp70/HspA1A in cell lysates. ..



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