Clone Assay:Article Title: Tumour markers
Article Snippet: .. Method Commercially available cDNA for p53 (E. coli clone pBH53, deposited in the American Type Culture Collection under accession number 79110) was cloned into the PinPointTM plasmid vector (Promega Corporation, Madison Wis., USA) using standard molecular biology techniques. .. The PinPoint™ vector is designed to facilitate the production of fusion proteins comprising a biotinylation domain (consisting of a fragment of a biotin carboxylase carrier protein) fused N-terminally to the target protein of interest.
Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino acids 927–1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791–1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D), and their products were cloned into pET28a via the engineered restriction sites using a standard molecular biology procedure as was used previously to clone the BhCBM6 gene fragment ( 5 ) (see supplemental Table S1 for primers). .. Gene fragments encoding BH0236Δ56 (residues 29–926) and BH0236 (residues 29–1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “In-Fusion HD Cloning” method (Clontech) (see supplemental Table S1 for primers).
Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino by guest on A ugust 23, 2017 http://w w w .jbc.org/ D ow nloaded from acids 927-1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791-1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D) and their products were cloned into pET28a via the engineered restriction sites using standard molecular biology procedure as was previously used to clone the BhCBM6 gene fragment (5) (see Table S1 for primers). .. Gene fragments encoding BH0236D56 (residues 29-926), and BH0236 (residues 29-1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “in fusion HD Cloning” method (Clonetech Laboratories Inc., Mountain View, CA) (see Table S1 for primers).
Article Title: Tumour markers
Article Snippet: .. Method Commercially available cDNA for p53 (E. coli clone pBH53, deposited in the American Type Culture Collection under accession number 79110) was cloned into the PinPointTM plasmid vector (Promega Corporation, Madison Wis., USA) using standard molecular biology techniques. .. The Pinpoint™ vector is designed to facilitate the production of fusion proteins comprising a biotinylation domain (consisting of a fragment of a biotin carboxylase carrier protein) fused N-terminally to the target protein of interest.
Plasmid Preparation:Article Title: Tumour markers
Article Snippet: .. Method Commercially available cDNA for p53 (E. coli clone pBH53, deposited in the American Type Culture Collection under accession number 79110) was cloned into the PinPointTM plasmid vector (Promega Corporation, Madison Wis., USA) using standard molecular biology techniques. .. The PinPoint™ vector is designed to facilitate the production of fusion proteins comprising a biotinylation domain (consisting of a fragment of a biotin carboxylase carrier protein) fused N-terminally to the target protein of interest.
Article Title: Tumour markers
Article Snippet: .. Method Commercially available cDNA for p53 (E. coli clone pBH53, deposited in the American Type Culture Collection under accession number 79110) was cloned into the PinPointTM plasmid vector (Promega Corporation, Madison Wis., USA) using standard molecular biology techniques. .. The Pinpoint™ vector is designed to facilitate the production of fusion proteins comprising a biotinylation domain (consisting of a fragment of a biotin carboxylase carrier protein) fused N-terminally to the target protein of interest.
other:Article Title: Genome-Wide Discovery of Putative sRNAs in Paracoccus denitrificans Expressed under Nitrous Oxide Emitting Conditions
Article Snippet: , Terrabacteria group , Chloroflexus Actinobacteria , Chloroflexus aurantiacus J-10-fl, Chloroflexus sp. Y-400-fl , Streptomyces sp. RTd22, Streptomyces sp. SAT1, Streptomyces iranensis, Streptomyces parvulus, Streptomyces ambofaciens, Streptomyces ambofaciens ATCC 23877, Streptomyces sp. S10(2016), Streptomyces albus J1074, Streptomyces reticuli, Streptomyces globisporus C-1027, Streptomyces sp. FR-008, Streptomyces hygroscopicus subsp. limoneus, Streptomyces hygroscopicus subsp. jinggangensis, Streptomyces sp. CFMR 7, Streptomyces pristinaespiralis, Streptomyces sp. PBH53 Streptomyces incarnatus, Streptomyces albulus, Streptomyces lydicus A02, Streptomyces nodosus, Streptomyces glaucescens, Streptomyces lividans TK24, Streptomyces davawensis JCM 4913, Micromonospora aurantiaca ATCC 27029, Thermobispora bispora DSM 43833, Amycolatopsis japonica, Modestobacter marinus, Blastococcus saxobsidens DD2..
Amplification:Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino acids 927–1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791–1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D), and their products were cloned into pET28a via the engineered restriction sites using a standard molecular biology procedure as was used previously to clone the BhCBM6 gene fragment ( 5 ) (see supplemental Table S1 for primers). .. Gene fragments encoding BH0236Δ56 (residues 29–926) and BH0236 (residues 29–1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “In-Fusion HD Cloning” method (Clontech) (see supplemental Table S1 for primers).
Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino by guest on A ugust 23, 2017 http://w w w .jbc.org/ D ow nloaded from acids 927-1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791-1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D) and their products were cloned into pET28a via the engineered restriction sites using standard molecular biology procedure as was previously used to clone the BhCBM6 gene fragment (5) (see Table S1 for primers). .. Gene fragments encoding BH0236D56 (residues 29-926), and BH0236 (residues 29-1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “in fusion HD Cloning” method (Clonetech Laboratories Inc., Mountain View, CA) (see Table S1 for primers).
Polymerase Chain Reaction:Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino acids 927–1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791–1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D), and their products were cloned into pET28a via the engineered restriction sites using a standard molecular biology procedure as was used previously to clone the BhCBM6 gene fragment ( 5 ) (see supplemental Table S1 for primers). .. Gene fragments encoding BH0236Δ56 (residues 29–926) and BH0236 (residues 29–1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “In-Fusion HD Cloning” method (Clontech) (see supplemental Table S1 for primers).
Article Title: Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of β-1,3-glucan
Article Snippet: .. Gene fragments encoding the BhCBM56 (amino by guest on A ugust 23, 2017 http://w w w .jbc.org/ D ow nloaded from acids 927-1021) and the tandem of CBMs, referred to as BhCBM6/56 (residues 791-1021), were amplified by PCR from B. halodurans genomic DNA (ATCC BAA-125D) and their products were cloned into pET28a via the engineered restriction sites using standard molecular biology procedure as was previously used to clone the BhCBM6 gene fragment (5) (see Table S1 for primers). .. Gene fragments encoding BH0236D56 (residues 29-926), and BH0236 (residues 29-1021) were amplified by PCR and inserted in pET28a vector using primers designed for the “in fusion HD Cloning” method (Clonetech Laboratories Inc., Mountain View, CA) (see Table S1 for primers).
Inhibition:Article Title: Lactic acid bacteria: prominent player in the fight against human pathogens.
Article Snippet: Introduction: The human microbiome is a unique repository of diverse bacteria.. Over 1000 microbial species reside in the human gut, which predominantly influences the host’s internal environment and plays a significant role in host health.. Lactic acid bacteria have long been employed for multiple purposes, ranging from food to medicines.
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