htnfr2 (R&D Systems)
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htnfr2
Htnfr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/726-R2/Recombinant+Human+TNF+RII%2FTNFRSF1B+Fc+Chimera+Protein%2C+CF/pm34699840-53-20-21
Average 94 stars, based on 14 article reviews
Htnfr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/726-R2/Recombinant+Human+TNF+RII%2FTNFRSF1B+Fc+Chimera+Protein%2C+CF/pm34699840-53-20-21
Average 94 stars, based on 14 article reviews
htnfr2 - by Bioz Stars,
2026-11
94/100 stars
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1) Product Images from "Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies."
Article Title: Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies.
Journal: Journal of immunological methods
doi: 10.1016/j.jim.2021.113173
Figure Legend Snippet: Fig. 2. Characterization of anti-mTNFR2 mAbs targeting CRDs 1–4. (A) Schematic representation of the 6 mouse-human TNF2 chimeras CRD1-CRD4 (Cystein Rich Domain). (B) The targeting CRD of each mAb were determined by cell ELISA with mouse-human TNFR2 domain swap mutants. Data represented as a three-parameter OD450–620 detection based on mean and SD of three independent experiments. (C) The domain epitopes of the 13 mAbs are indicated on a hTNFR2-hTNFα trimer structure (PDB: 3ALQ), 74% similar to mouse TNFR2. The CRDs for one TNFR2 receptor are shown in indicated colors.
Techniques Used: Enzyme-linked Immunosorbent Assay
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For the second inhibition ELISA, the extracellular domain of TNFRII fused to the Fc region of Article Title: Anti-TNF-α-antibodies and functional fragments thereof Article Snippet: For the first inhibition ELISA, the extracellular domain of TNFRI fused to the Fc region of human IgG (R&D Systems, Cat. No. 372-RI) was coated on a 96-well Maxisorp ELISA at a concentration of 0.5 μg/mL. .. For the second inhibition ELISA, the extracellular domain of TNFRII fused to the Fc region of Enzyme-linked Immunosorbent Assay:Article Title: Anti-TNFα-antibodies and functional fragments thereof Article Snippet: L929 cells were sensitized to TNFα-induced apoptosis by addition of 1 pg/mL actinomycin D. Three-fold serial dilutions of anti-TNFα reference antibody or scFvs (3000-0.05 ng/mL) and 5 pM recombinant human TNFα (Peprotech, Cat. No. 300-01) were pre-incubated at room temperature for 1 hour. .. 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For the second inhibition ELISA, the extracellular domain of TNFRII fused to the Fc region of Article Title: Anti-TNF-α-antibodies and functional fragments thereof Article Snippet: For the first inhibition ELISA, the extracellular domain of TNFRI fused to the Fc region of human IgG (R&D Systems, Cat. No. 372-RI) was coated on a 96-well Maxisorp ELISA at a concentration of 0.5 μg/mL. .. 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In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 μg / ml) ( Recombinant:Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification Article Snippet: Cells were stimulated with ultrapure LPS (5 ng/ml unless specified otherwise) (E.coli 0111:B4, Invitrogen), recombinant human IFN-γ (2 ng/ml) (285-IF-100, R&D Systems), recombinant human TNFα (10 ng/ml) (10291-TA-020, R&D Systems), poly I:C (1μg / ml) (tlrI-pic, Invitrogen), R848 (1μg / ml) (tlrI-r848, Invitrogen), recombinant mouse IFN-γ (20 ng/ml) (485-MI-100, R&D Systems), recombinant mouse TNFα (5 ng/ml). (410-MT-010/CF, R&D Systems), recombinant mouse IL1b (20 ng/ml) (401-ML-005/CF, R&D Systems) at indicated time points and concentrations. .. In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 μg / ml) ( Article Title: Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies. Article Snippet: .. Immunoreactivity to mouse TNFR2 and cross-reactivity to human TNFR2 was assessed by ELISA using recombinant mTNFR2/Fc-protein (R&D Systems, 9707-R2) and Activation Assay:Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification Article Snippet: HUVECs were obtained from Lonza and cultured in EGM-2 Endothelial cell growth medium-2 bullet kit (Lonza, CC-3162) supplemented with penicillin-streptomycin and glutamine on gelatin coated plates (Millipore Sigma, ES-006-B). .. For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of Incubation:Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification Article Snippet: HUVECs were obtained from Lonza and cultured in EGM-2 Endothelial cell growth medium-2 bullet kit (Lonza, CC-3162) supplemented with penicillin-streptomycin and glutamine on gelatin coated plates (Millipore Sigma, ES-006-B). .. For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of other:Article Title: Anti-TNF-alpha-antibodies and functional fragments thereof Article Snippet: Data were analyzed using a four-parameter logistic curve fit using the Softmax Data Analysis Software (Molecular Devices), and the concentration of reference antibody or scFvs required to neutralize TNFα-induced apoptosis by 50% and 90% (IC50 and IC90) was calculated (see also FIG. 7). |