anti coup tfii (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti coup tfii
Anti Coup Tfii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/6434s/COUP-TFII+Rabbit+mAb/pm40407141-111-35-37
Average 93 stars, based on 23 article reviews

anti coup tfii - by Bioz Stars, 2026-10

93/100 stars

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Blocking Assay:

Article Title: A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†.
Article Snippet: The Cre/loxP system is widely used for site-specific genetic manipulation in mice.. The PgrCre mouse model, where Cre recombinase is driven by the progesterone receptor promoter, is commonly used for gene ablation in Pgr -positive uterine cells.. However, the PgrCre is active in the neonatal uterus and does not allow temporal control.

Virus:

Article Title: Differentiation of lung tissue-resident c-Kit + cells into microvascular endothelial cells alleviates pulmonary vascular remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-alpha smooth muscle Actin Abcam Cat# ab5694; RRID: AB_2223021 Anti-human c-KIT Abcam Cat# ab115801; RRID: AB_10898464 Anti-mouse c-Kit R&D systems Cat# AF1356; RRID: AB_354750 Anti-mouse CD31 BD Pharmingen Cat# 550274; RRID: AB_393571 Anti-human CD31 Abcam Cat# ab9498; RRID: AB_307284 Anti-mouse CD45 eBioscience Cat# 14-0451-81; RRID: AB_467250 Anti-human CD45 Proteintech Cat# 60287-1-Ig; RRID: AB_2881404 Anti-mouse CD140a eBioscience Cat# 14-1401-82; RRID: AB_467491 Anti-tdTomato Rockland Cat# 600-906-379; RRID: AB_2614816 Anti-GFP Abcam Cat# ab290; RRID: AB_303395 Anti-Sftpc Abcam Cat# ab211326; RRID: AB_2927746 Anti-Pdpn Abcam Cat# ab109059; RRID: AB_2848181 Anti-E-cadherin Cell Signaling Technology Cat# 3195S; RRID: AB_2291471 Anti-Scgb1a1 Proteintech Cat# 10490-1-AP; RRID: AB_2183285 Anti-Ednrb Proteintech Cat# 20964-1-AP; RRID: AB_2878774 Anti-Connexin 40 Invitrogen Cat# 36-5000; RRID: AB_2533264 Anti-EphB4 Abcam Cat# ab254301; RRID: AB_2892199 Anti-Nr2f2 Abcam Cat# ab211777; RRID: AB_2895604 Anti-c-Kit Cell Signaling Technology Cat# 3074S; RRID: AB_1147633 Anti-Scf Abcam Cat# ab64677; RRID: AB_1861346 Anti-Notch1 Abcam Cat# ab52627; RRID: AB_881725 Anti-Foxf1 R&D systems Cat# AF4798; RRID: AB_2105588 Anti-Nr2f2 Cell Signaling Technology Cat# 6434S; RRID: AB_11220428 Anti-DTR Abcam Cat# ab66792; RRID: AB_1951275 Anti-CD45 FITC eBioscience Cat# 11-0451-85; RRID: AB_465051 Anti-CD45 PE BioLegend Cat# 103106; RRID: AB_312971 Anti-CD31 PE-Cy7 eBioscience Cat# 25-0311-82; RRID: AB_2716949 Anti-CD140a PE BioLegend Cat# 135905; RRID: AB_1953268 Anti-c-Kit APC eBioscience Cat# 17-1171-82; RRID: AB_469430 Anti-Sca1 FITC eBioscience Cat# 11-5981-82; RRID: AB_465333 Anti-c-Kit PE BioLegend Cat# 105807; RRID: AB_313216 Bacterial and virus strains AAV ENT-CMV bGlobin-FLEX-MCSEGFP-WPRE-hGH polyA Genechem N/A AAV ENT- CMV bGlobin-FLEX-EGFPMIR155(mcs)-WPRE-hGH polyA Genechem N/A Biological samples Human lung tissues: non-PH donors China-Japan Friendship Hospital Lung Transplantation Center N/A Human lung tissues: PH patients China-Japan Friendship Hospital Lung Transplantation Center N/A Chemicals, peptides, and recombinant proteins Tamoxifen Sigma-Aldrich Cat# T5648 Corn oil Solarbio Cat# C7030 Diphtheria toxin Sigma-Aldrich Cat# D0564 (Continued on next page) e1 Developmental Cell 60, 1–17.e1–e7, June 9, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER SU5416 Sigma-Aldrich Cat# S8442 Tribromoethanol Sigma-Aldrich Cat# 75-80-9 Red blood cell lysis buffer Solarbio Cat# R1010 DMEM Solarbio Cat# 11995 EDTA Antigen Retrieval Solarbio Cat# C1034 Glycerinated gelatin Beyotime Cat# C0187 Optimum cutting temperature Sakura Cat# 4583 DAPI BD Pharmingen Cat# 564907 Mounting Medium with DAPI Abcam Cat# ab104139 Goat serum ZSGB-BIO Cat# ZLI-9056 Collagenase I Sigma-Aldrich Cat# C0130 Elastase Sigma-Aldrich Cat# E1250 Neutral protease Worthington Cat# 9001-92-7 Deoxyribonuclease I Roche Cat# 10104159001 Fetal Bovine Serum Gibco Cat# 10099141C Benzyl alcohol Solarbio Cat# B7710 Benzyl benzoate Solarbio Cat# B8460 RIPA buffer Beyotime Cat# P0013B PMSF Solarbio Cat# P0100 Protease/phosphatase cocktail Cell Signaling Technology Cat# 58725 Skim milk BD biosciences Cat# 232100 TRIzol Reagent Invitrogen Cat# 15596018CN TransStart Tip Green qPCR SuperMix TransGen Biotech Cat# AQ141 Critical commercial assays AEC Horseradish Peroxidase Color Development Kit ZSGB-BIO Cat# ZLI-9037 Rabbit polymer detection system ZSGB-BIO Cat# PV-6001 Goat enhanced polymer detection system ZSGB-BIO Cat# PV-9003 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 ECL Detection Reagent YEASEN Cat# 36208ES76 Masson Tricolor Staining Kit Servicebio Cat# G1006 FastKing Reverse Transcription Kit TIANGEN Cat# KR116 Deposited data Raw scRNA-seq data of control and SuHx lung from C57BL/6J mice This paper GSA: CRA006636 Raw scRNA-seq data of lung tdTomato+ cell from Kit-CreER;Rosa26-tdTomato mice This paper GSA: CRA020619 Human lung scRNA-seq data (EGAS00001004344) European Genome-phenome Archive (EGA)13 https://ega-archive.org/studies/ EGAS00001004344 Mouse reference genome, mm10 (GENCODE vM23) GENCODE https://www.gencodegenes.org/ mouse/release_M23.html Raw full blots data This paper https://data.mendeley.com/ datasets/3xp6n37v2f/1 Experimental models: Organisms/strains Mouse: C57BL/6J Beijing Vital River Laboratory Animal Technology Strain# 219 Mouse: Kit-CreER Kindly provided by Dr. Bin Zhou.11,12 N/A Mouse: Rosa26-LSL-tdTomato: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J The Jackson Laboratory Strain# 007909 (Continued on next page) Developmental Cell 60, 1–17.e1–e7, June 9, 2025 e2

Bioprocessing:

Transplantation Assay:

Recombinant:

SDS Page:

Article Title: Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptor.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS 0.1%) and quantified with the PierceTM BCA Protein Assay Kit (Invitrogen, ThermoFisher-SCIENTIFIC). .. Protein samples were subjected to SDS-PAGE and Western blot analyses using the following antibodies: anti-NR2F2 (Cell Signaling Technology, #6434), anti-NR2F6 (Proteintech, clone 2H2B8, #60117-2-PBS), anti-p65 Ser536 (NFKB) (Cell Signaling Technology, #3033), anti-p65 (NFKB) (Cell Signaling Technology, #8242), anti-STAT1 Tyr701 (Cell Signaling Technology, #9167), anti-IRF1 (Cell Signaling Technology, #8478), anti-RIP1 Ser166 (Cell Signaling Technology, #65746), anti-RIP1 (Cell Signaling Technology, #73271), anti-MLKL (Cell Signaling Technology, #26539), primary antibodies and appropriate fluorescence-tagged secondary antibodies [Cy3-conjugated AffiniPure goat anti-mouse IgG (H + L), #115-165- 003 and Cy5-conjugated AffiniPure goat anti-rabbit IgG (H + L) #111-175-144, Jackson ImmunoResearch]. ..

Western Blot:

Article Title: Differentiation of lung tissue-resident c-Kit + cells into microvascular endothelial cells alleviates pulmonary vascular remodeling.
Article Snippet: After being blocked in 5% skim milk (232100, BD Biosciences) at RT for 1 h, PVDF membranes were incubated in primary antibodies at 4 C overnight and then treated with HRP-conjugated secondary antibodies at RT for 1 h. Membranes were finally visualized by ECL Detection Reagent (36208ES76, YEASEN) and detected on a chemiluminescence image analysis system (Tanon-5200, Tanon). .. Primary antibodies used in western blot analysis were as listed: anti-c-Kit (3074S, CST, 1:1000), anti-Nr2f2 (6434S, CST, 1:1000), anti-DTR (ab66792, Abcam, 1:1000), anti-Scf (ab64677, abcam, 1:1000), anti-b-Actin (AC026, Abclonal, 1:5000). ..

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