5x protoscript rt buffer (New England Biolabs)

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New England Biolabs 5x protoscript rt buffer
5x Protoscript Rt Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+protoscript+rt+buffer/ProtoScript+II+Reverse+Transcriptase/pm40204698-333-23-27
Average 97 stars, based on 1435 article reviews

5x protoscript rt buffer - by Bioz Stars, 2026-09

97/100 stars

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Isolation:

Article Title: Decoding the BRCA2 reversion principles underlying PARP inhibitor resistance
Article Snippet: .. RNA was isolated using the RNeasy Mini Kit (74104, Qiagen) with on-column DNase treatment where indicated. cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit using the standard protocol with d(T)23VN primers (M0368, NEB). .. Regions spanning candidate inter-exonic or splicing-mediated reversion events were amplified using the specified primers in flanking exons, with Q5 polymerase in 25 μL reactions containing 2 μL of cDNA diluted to 1:50 (M0493S, NEB).

Synthesized:

Reverse Transcription:

Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
Article Snippet: The RNA of the RNA-DNA was fragmented in buffer (4 μL of 5× Protoscript II buffer, M0368S, NEB; 1 μL of 10 mM dNTPs) with a primer complementary to the adaptor oligo (5’CCGCTTATCGAACTGCTG, 20 pmol per reaction) at 94 °C for 6 min prior to reverse transcription. .. The RNA-DNA was reverse transcribed using ProtoScript II Reverse Transcriptase (200 units of M0368S, NEB) which was mixed into each reaction after the addition of DTT (10 mM final) and murine RNase Inhibitor (8 units) to each sample. ..

Article Title: Host GPCR-cAMP signaling balances Gαs and Gαi activity to control intracellular Brucella infection.
Article Snippet: Total RNA was extracted using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DNase treatment. .. One microgram of RNA was reverse-transcribed using ProtoScript II Reverse Transcriptase (NEB). .. Quantitative PCR was performed using DyNAmo HS SYBR 2X Master Mix (Thermo Scientific) on a Bio-Rad CFX384 Real-Time PCR Detection System.

Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19
Article Snippet: Total RNA was purified with the RNeasy Plus kit (74134, Qiagen) or Direct-zol DNA/RNA Miniprep (R2080, Zymo Research). .. Reverse transcription was performed using Protoscript II reverse transcriptase (M0368, New England Biolabs) with random primers. cDNA was amplified with 2X Forget-Me-Not EvaGreen qPCR Master Mix with Low ROX (31045, Biotium) and primers against mouse TNFα, IL-1β, IL-6, MCP1, CXCL1, IFN-γ, Poldip2 and human ACE2 ( ). .. Reactions were carried out in 96-well qPCR plates (4346907, Thermo Fisher Scientific), using a QuantStudio 7 Flex (Thermo Fisher Scientific) Real-Time qPCR System.

Article Title: The conserved nematode pheromone ascr#18 primes plant immunity.
Article Snippet: DNA contamination was removed by treatment with DNase I (Invitrogen, USA). .. First-strand cDNA was synthetized from 50 ng of mRNA using ProtoScript II Reverse Transcriptase (NEB, USA). .. The qRT-PCR assay was carried out in CFX96 Real-Time System (Bio-Rad, USA), and transcripts were amplified using iTaqTM Universal SYBR Green Supermix (Bio-Rad) from 1 μL of 20X diluted cDNA in a total of 20 μL reaction using 1 μL of each 10 μM gene-specific primers (see Supplementary Table S3).

Article Title: Unmasking the common enemy: drug resistance mechanisms across three different EGFR inhibitor generations are associated with co-targetable alterations in extracellular matrix signaling.
Article Snippet: Droplet digital PCR (ddPCR) oils and ddPCR supermix for probes (no dUTP) were obtained from Bio-Rad Laboratories (Hercules, CA, USA). .. Q5® Hot Start High-Fidelity DNA Polymerase (supplied with 5X Q5® Reaction Buffer and 5X Q5® High GC Enhancer), ProtoScript® II Reverse Transcriptase and Deoxynucleotide (dNTP) Solution Mix (for ddPCR assay) were obtained from New England Biolabs (Ipswich, MA, USA). .. NucleoSpin Gel and PCR Clean‐up Kit was purchased from Macherey-Nagel (Düren, Germany).

Article Title: FvTFL1 reverses the function of FvGI-FvCO-FvFT1 pathway in the photoperiodic flowering of woodland strawberry
Article Snippet: .. Protoscript II reverse transcriptase kit (NEB, US) was used for cDNA synthesis. qRT-PCR was performed using a LightCycler 480 SYBR Green I Master kit (Roche Diagnostics, 555 Indianapolis, US) in a Roche LightCycler 480 (Roche Diagnostics, Indianapolis US), as described in Mouhu et al ., (2009) . ..

Amplification:

Real-time Polymerase Chain Reaction:

cDNA Synthesis:

Quantitative RT-PCR:

SYBR Green Assay:

other:

Article Title: Near‐Source Wastewater Surveillance of SARS‐CoV‐2, Influenza A, and Respiratory Syncytial Virus With Spatiotemporal Evaluation of Pepper Mild Mottle Virus as a Fecal Indicator
Article Snippet: The protocol was modified to process 0.3 mL of concentrate with 0.6 mL of lysis buffer, yielding a 0.1‐mL elution. cDNA synthesis followed the NEB #M0368 protocol (New England Biolabs, MA, USA) with dNTP and random hexamer volumes specified in Table .

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