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non reducing 4x loading buffer  (Bio-Rad)


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    Bio-Rad non reducing 4x loading buffer
    Non Reducing 4x Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+loading+buffer/4x+Laemmli+Sample+Buffer/pm41991007-206-24-28
    Average 99 stars, based on 11708 article reviews
    non reducing 4x loading buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Vaccination modality and injection site influence the immune response and protection against sea lice Lepeophtheirus salmonis infestation in Atlantic salmon ( Salmo salar )
    Article Snippet: Albumin depletion was performed prior to Western blot using PierceTM Albumin Serum Depletion Kits (Thermo Scientific), following the manufacturer's protocol. .. For the reduced samples, 30 μL of purified and diluted plasma (1:20 in PBS) from an Atlantic salmon was mixed with 15 μL of 4x Laemmli Sample Buffer (Bio-Rad) and NuPAGE sample reducing agent (Invitrogen). ..

    Clinical Proteomics:

    Article Title: Vaccination modality and injection site influence the immune response and protection against sea lice Lepeophtheirus salmonis infestation in Atlantic salmon ( Salmo salar )
    Article Snippet: Albumin depletion was performed prior to Western blot using PierceTM Albumin Serum Depletion Kits (Thermo Scientific), following the manufacturer's protocol. .. For the reduced samples, 30 μL of purified and diluted plasma (1:20 in PBS) from an Atlantic salmon was mixed with 15 μL of 4x Laemmli Sample Buffer (Bio-Rad) and NuPAGE sample reducing agent (Invitrogen). ..

    Transfection:

    Article Title: Consequences of human genetic variations in KIAA0319L , encoding adeno-associated virus receptor, on AAV-mediated gene transfer
    Article Snippet: The protein concentrations of cell lysates were determined using a Pierce BCA Protein Assay Kit. .. A total of 10 μg of cell protein lysate from transfected HeLa KIAA0319L −/− cells was added to one-fourth volume of 4X sample buffer with 2-mercaptoethanol (1610747, Bio-Rad, Hercules, CA) and incubated at 55°C for 30 min while shaking. .. Proteins were separated using NuPAGE Bis-Tris Mini Protein Gels, 4%–12% gradient, 1.5 mm thick (#NP0335, Thermo Scientific, Waltham, MA), using 1X NuPAGE MOPS SDS Running Buffer (#NP0001, Thermo Scientific, Waltham, MA) with one lane containing 5 μL of Precision Plus Protein Dual Color Standards (#1610394, Bio-Rad, Hercules, CA).

    Incubation:

    Article Title: Consequences of human genetic variations in KIAA0319L , encoding adeno-associated virus receptor, on AAV-mediated gene transfer
    Article Snippet: The protein concentrations of cell lysates were determined using a Pierce BCA Protein Assay Kit. .. A total of 10 μg of cell protein lysate from transfected HeLa KIAA0319L −/− cells was added to one-fourth volume of 4X sample buffer with 2-mercaptoethanol (1610747, Bio-Rad, Hercules, CA) and incubated at 55°C for 30 min while shaking. .. Proteins were separated using NuPAGE Bis-Tris Mini Protein Gels, 4%–12% gradient, 1.5 mm thick (#NP0335, Thermo Scientific, Waltham, MA), using 1X NuPAGE MOPS SDS Running Buffer (#NP0001, Thermo Scientific, Waltham, MA) with one lane containing 5 μL of Precision Plus Protein Dual Color Standards (#1610394, Bio-Rad, Hercules, CA).

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: Next, the total protein content was measured using a PierceTM BCA Protein Assay Kit (ThermoFisher Scientific, Ireland) according to the manufacturer's instructions. .. Thereafter, 15 μg total protein was incubated at 95 °C with 4x Laemmli sample loading buffer (BioRad) before loading into a gradient gel (Mini-PROTEAN TGX Gels 4–20 %, BioRad). .. Proteins were separated by electrophoresis and subsequently transferred to a polyvinylidene fluoride or polyvinylidene difluoride (Trans-Blot Turbo Transfer - 0.2 μm PVDF, BioRad) membrane and blocked with 5 % (w/v) fat-free milk in 0.1 % (v/v) tris-buffered saline (TBS)-Tween 20 (TBST) (Sigma-Aldrich, Ireland).

    Protein Concentration:

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. The protein concentration was adjusted to 1 mg/mL using 4X sample buffer (BIO-RAD, cat# 1610747) containing 10% 2-mercaptoethanol (Sigma Aldrich, cat# 21985023). ..



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    Image Search Results


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    Journal: bioRxiv

    Article Title: Mechanism of K63-linked polyubiquitin recognition and cleavage by the BRCA1-A complex

    doi: 10.64898/2026.06.05.730395

    Figure Lengend Snippet: a , Domain architecture of RAP80 and ARISC constructs. FL, full-length; SIM, small ubiquitin-like modifier (SUMO)-interacting motif; UIM, ubiquitin-interacting motif; AIR, Abraxas1-interacting region; ZnF, zinc finger; MPN, Mpr1, Pad1 N-terminal; CC, coiled coil; UEV, ubiquitin E2 variant; vWFA, von Willebrand factor type A ( left ). Schematics of indicated complexes ( right ). b , SDS-PAGE analysis of ARISC, ARISC–RAP80, and ARISC–RAP80 AIR. c , K63-linked ubiquitin chains (1 µM) were incubated with ARISC or ARISC–RAP80 (5 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. d , K63-Ub2, -Ub4, and - Ub7 chains (1 µM) were incubated with ARISC, ARISC–RAP80, or ARISC–RAP80 AIR (5 nM) for the indicated time points. Cleavage activity was analysed as in c . Data are representative of three independent experiments. e , Schematics ( left ) and SDS-PAGE analysis ( right ) of indicated complexes. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. f , Alexa-Fluor 488 (AF488) labelled distally (AF488- Cys Ub4 K63R ) blocked K63-Ub4 chains (1.5 µM) were incubated with ARISC–RAP80, ARISC–RAP80 ΔUIMs, or ARISC–RAP80 ΔZnF (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and fluorescence scanning ( left ; see Methods ). The disappearance of the K63-Ub4 parent band was quantified using densitometry, and plotted as fraction of substrate consumed (%). Data points are mean ± SEM of two independent experiments ( right ). g , Cyclical and linear K63-Ub5 chains (2 µM) were incubated with ARISC or ARISC–RAP80 (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and Oriole staining. Data are representative of two independent experiments. Ub, ubiquitin; DUB, deubiquitylating enzyme. * indicates lower molecular weight ubiquitin species.

    Article Snippet: Reactions were stopped with the addition of 3 μL 4x SDS-PAGE loading dye [240 mM Tris-HCl pH 6.8, 40% (v/v) glycerol, 8% (w/v) SDS, 0.04% (w/v) bromophenol blue, and 5% (v/v) β-Mercaptoethanol], and products were separated on 4-12% or 12% Nu-PAGE Bis-Tris gels (Invitrogen).

    Techniques: Construct, Ubiquitin Proteomics, Variant Assay, SDS Page, Incubation, Activity Assay, Silver Staining, Fluorescence, Staining, Molecular Weight

    a , K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or the indicated ARISC variants (5 nM) for 60 minutes. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. b, SDS-PAGE analysis of ARISC(E33A)–RAP80, ARISC(E33A) BRCC36(S98K) –RAP80, ARISC(E33A) Abraxas1(Δ42-55) –RAP80, and ARISC(E33A) BRCC45(ΔLoop) –RAP80. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. c, Spectral shift assays measuring binding of labelled ARISC(E33A)–RAP80 or the indicated mutant complexes (40 nM) to cyclical K63-Ub6 chains (20 µM-0 µM). Data points are mean ± SEM of two independent experiments carried out in technical duplicates. Dissociation constants (K d ) are indicated; CI, confidence interval. d, Representative images of WT or mutants BRCC36 IRIF in HT-29 cells 4 h post irradiation (10 Gy). Scale bar is 10 µm. e, Western blots showing BRCC36 protein levels in HT-29 cells reconstituted with WT or mutants BRCC36 as indicated (l eft ). Scatter plot showing quantification of the BRCC36 IRIF described in d . Data represent mean ± SEM derived from n ≥ 300 nuclei examined over two independent experiments; p values are indicated, unpaired two-tailed t test ( right ). f, K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or ARISC Δ42-55 (Abraxas1 Δ42-55) (5 nM) for up to 60 minutes. Cleavage activity was analysed as in a . Data are representative of two independent experiments. DUB, deubiquitylating enzyme; WT, wild type; Ub, ubiquitin.

    Journal: bioRxiv

    Article Title: Mechanism of K63-linked polyubiquitin recognition and cleavage by the BRCA1-A complex

    doi: 10.64898/2026.06.05.730395

    Figure Lengend Snippet: a , K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or the indicated ARISC variants (5 nM) for 60 minutes. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. b, SDS-PAGE analysis of ARISC(E33A)–RAP80, ARISC(E33A) BRCC36(S98K) –RAP80, ARISC(E33A) Abraxas1(Δ42-55) –RAP80, and ARISC(E33A) BRCC45(ΔLoop) –RAP80. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. c, Spectral shift assays measuring binding of labelled ARISC(E33A)–RAP80 or the indicated mutant complexes (40 nM) to cyclical K63-Ub6 chains (20 µM-0 µM). Data points are mean ± SEM of two independent experiments carried out in technical duplicates. Dissociation constants (K d ) are indicated; CI, confidence interval. d, Representative images of WT or mutants BRCC36 IRIF in HT-29 cells 4 h post irradiation (10 Gy). Scale bar is 10 µm. e, Western blots showing BRCC36 protein levels in HT-29 cells reconstituted with WT or mutants BRCC36 as indicated (l eft ). Scatter plot showing quantification of the BRCC36 IRIF described in d . Data represent mean ± SEM derived from n ≥ 300 nuclei examined over two independent experiments; p values are indicated, unpaired two-tailed t test ( right ). f, K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or ARISC Δ42-55 (Abraxas1 Δ42-55) (5 nM) for up to 60 minutes. Cleavage activity was analysed as in a . Data are representative of two independent experiments. DUB, deubiquitylating enzyme; WT, wild type; Ub, ubiquitin.

    Article Snippet: Reactions were stopped with the addition of 3 μL 4x SDS-PAGE loading dye [240 mM Tris-HCl pH 6.8, 40% (v/v) glycerol, 8% (w/v) SDS, 0.04% (w/v) bromophenol blue, and 5% (v/v) β-Mercaptoethanol], and products were separated on 4-12% or 12% Nu-PAGE Bis-Tris gels (Invitrogen).

    Techniques: Incubation, Activity Assay, SDS Page, Silver Staining, Binding Assay, Mutagenesis, Irradiation, Western Blot, Derivative Assay, Two Tailed Test, Ubiquitin Proteomics