atomic force microscopy (Bruker Corporation)

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Bruker Corporation atomic force microscopy

Attenuation of chondrocyte degeneration by MSC exosomes. (A) Morphology of exosomes imaged by TEM. (B) Western blotting of the exosome markers TSG101, CD9, and CD63. (C) Particle size distribution of exosomes. (D) Representative immunofluorescence micrograph of DiO (green)-labeled exosomes taken up by chondrocytes. Scale bar = 100 μm. (E) Confocal <t>microscopy</t> of chondrocytes incubated with DiO-labeled exosomes (green) for 48 h, MitoTracker-labeled mitochondria (red), and exosomes colocalized with mitochondria (yellow). Scale bar = 20 μm. (F, G) Immunofluorescence staining and quantification of MMP13, COL2A1, ADAMTS-5, and aggrecan in chondrocytes treated with IL-1β or IL-1β + exosomes. n = 5. *p < 0.05; **p < 0.01.

Atomic Force Microscopy, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 10242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+microstructures+visualization/MultiMode+8/pmc06525998-73-0-6
Average 99 stars, based on 10242 article reviews

atomic force microscopy - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration"

Article Title: Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration

Journal: Theranostics

doi: 10.7150/thno.31017

Figure Legend Snippet: Attenuation of chondrocyte degeneration by MSC exosomes. (A) Morphology of exosomes imaged by TEM. (B) Western blotting of the exosome markers TSG101, CD9, and CD63. (C) Particle size distribution of exosomes. (D) Representative immunofluorescence micrograph of DiO (green)-labeled exosomes taken up by chondrocytes. Scale bar = 100 μm. (E) Confocal microscopy of chondrocytes incubated with DiO-labeled exosomes (green) for 48 h, MitoTracker-labeled mitochondria (red), and exosomes colocalized with mitochondria (yellow). Scale bar = 20 μm. (F, G) Immunofluorescence staining and quantification of MMP13, COL2A1, ADAMTS-5, and aggrecan in chondrocytes treated with IL-1β or IL-1β + exosomes. n = 5. *p < 0.05; **p < 0.01.

Techniques Used: Western Blot, Immunofluorescence, Labeling, Confocal Microscopy, Incubation, Staining

3D printing of radially oriented ECM/GelMA/exosome bioink. (A) Illustration of the SLA printing process. The printing resolution was set to 0.05 mm. (B) CAD file, printing process, and scaffold (stained with methylene blue dye) printed by the 3D printer. The scaffold was observed under microscopy and SEM. (C) The exosome retention ratio of the scaffold for 14 days. (D) In vivo fluorescence imaging of exosomes in PBS and in the 3D printed scaffold. Both groups of exosomes were labeled with Vybrant DiO (green) dye before implantation.

Figure Legend Snippet: 3D printing of radially oriented ECM/GelMA/exosome bioink. (A) Illustration of the SLA printing process. The printing resolution was set to 0.05 mm. (B) CAD file, printing process, and scaffold (stained with methylene blue dye) printed by the 3D printer. The scaffold was observed under microscopy and SEM. (C) The exosome retention ratio of the scaffold for 14 days. (D) In vivo fluorescence imaging of exosomes in PBS and in the 3D printed scaffold. Both groups of exosomes were labeled with Vybrant DiO (green) dye before implantation.

Techniques Used: Staining, Microscopy, In Vivo, Fluorescence, Imaging, Labeling

other:

Article Title: Advancing cellulose nanofiber filament technology: Thermal drying effects on strength and morphology.
Article Snippet: Before measurements, TCNF gel was diluted with DI water to 0.01 and 0.005 wt%, homogenized by Ultra Turrax (IKA 18D, Sweden) for 10 min at 10000 rpm.

Article Title: Abrupt eruptive instability of ice adhered to solid surfaces
Article Snippet: The hydrophobic surfaces were masked with metal and exposed to ultraviolet (UV) light (PSD-UV4, Novascan; 2 hours).

Imaging:

Article Title: Amelogenin proteolysis orchestrates functional amyloid pathways in enamel development
Article Snippet: .. AFM imaging was conducted on a Bruker Multimode 8 with a Nanoscope V controller (Bruker) in ScanAsyst air mode using a silicon probe (Bruker ScanAsyst Air-HR). .. A 20 μL aliquot of the sample solution was deposited onto a freshly cleaved mica substrate (Electron Microscopy Sciences) and incubated for 30 min in a wet cell for immobilization.

Article Title: Structural characterization and antitumor activity via immune modulation of an α-glucan from Grifola frondosa
Article Snippet: Grifola frondosa is a valued edible and medicinal fungus whose polysaccharides are key bioactive components, yet the identification of specific homogeneous effectors and their detailed immunomodulatory mechanisms remains a critical focus of research.. In this study, polysaccharide fractions were isolated from G. frondosa fruiting bodies via systematic purification, and the principal bioactive polysaccharide responsible for breast cancer inhibition was identified.. A novel homogeneous polysaccharide, GFI-21α, was determined as the primary effector.

Microscopy:

Article Title: Advancing cellulose nanofiber filament technology: Thermal drying effects on strength and morphology.
Article Snippet: The surface morphologies of the filaments were evaluated by Atomic Force Microscope (AFM), Bruker Multimode 8 (USA), in the ScanAsystAir mode. .. The surface morphologies of the filaments were evaluated by Atomic Force Microscope (AFM), Bruker Multimode 8 (USA), in the ScanAsystAir mode. .. The cantilever was made of a silicon tip on a nitride lever with the back side coated with reflective aluminium, resonance frequency range: 70 kHz, spring constant: 0.4 N/m (Bruker, USA).

Article Title: Abrupt eruptive instability of ice adhered to solid surfaces.
Article Snippet: .. Surface morphologies were characterized using an atomic force microscope (MultiMode 8, Bruker) in ScanAsyst mode in air. ..

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