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3d  (Oxford Instruments)


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    Structured Review

    Oxford Instruments 3d
    A-D. Each circle is color coded for each embryo imaged, and overlaid onto box plots. A. Total number of AO+ cells observed during live imaging from ~54-62 hours post fertilization (hpf). B. The percent of AO+ cells that were observed to be engulfed by a microglial cell during the imaging session. C. For AO+ cells observed to be cleared <t>by</t> <t>microglia,</t> the percent showing association with a microglial cell at the onset of AO+ signal. D. Each circle represents individual cells from each embryo that was imaged. A selected number of individual AO+ cells, which were observed to appear and be cleared, during the imaging session were tracked in each embryo imaged. The duration of AO signal is shown for these individual AO+ cells. E-F represent max projections of selected, flattened z planes over a selected time-lapse. E. Shows the initial detection of an AO+ cell (green, indicated by red-orange arrow) associated with a microglial cell (microglia=magenta). E’. Shows the same AO+ cell (green, indicated by the magenta arrow) 80 minutes later, just before the AO signal is lost, after it had moved with the migrating microglial cell (magenta). F. The movement of the AO+ cell body was temporally color-coded to show its dynamic movement over time; the AO+ cell was associated with a microglial cell at all time frames. The color scale on the right indicates progression of time, where the first frame corresponds to 0:00 and the last frame is 85:00 (min:sec). Scale bar in bottom right of F = 5 microns and applies to E-F. G and H. To show a representation of dynamics of AO+ cell movement in association with microglia, a subset of AO+ cells were tracked in <t>3D</t> using Imaris software. Quantifications show the speed (G) and total displacement (H) of individual AO+ cell bodies. Box plots are shown and circles are color coded to represent each embryo imaged. Each circle represents one AO+ cell that was tracked in each embryo that was imaged. Results are shown for n=6 different embryos.
    3d, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+tracking+algorithm/Imaris/pmc08714022-183-39-41
    Average 99 stars, based on 44673 article reviews
    3d - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Microglia in the developing retina couple phagocytosis with the progression of apoptosis via P2RY12 signaling"

    Article Title: Microglia in the developing retina couple phagocytosis with the progression of apoptosis via P2RY12 signaling

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    doi: 10.1002/dvdy.163

    A-D. Each circle is color coded for each embryo imaged, and overlaid onto box plots. A. Total number of AO+ cells observed during live imaging from ~54-62 hours post fertilization (hpf). B. The percent of AO+ cells that were observed to be engulfed by a microglial cell during the imaging session. C. For AO+ cells observed to be cleared by microglia, the percent showing association with a microglial cell at the onset of AO+ signal. D. Each circle represents individual cells from each embryo that was imaged. A selected number of individual AO+ cells, which were observed to appear and be cleared, during the imaging session were tracked in each embryo imaged. The duration of AO signal is shown for these individual AO+ cells. E-F represent max projections of selected, flattened z planes over a selected time-lapse. E. Shows the initial detection of an AO+ cell (green, indicated by red-orange arrow) associated with a microglial cell (microglia=magenta). E’. Shows the same AO+ cell (green, indicated by the magenta arrow) 80 minutes later, just before the AO signal is lost, after it had moved with the migrating microglial cell (magenta). F. The movement of the AO+ cell body was temporally color-coded to show its dynamic movement over time; the AO+ cell was associated with a microglial cell at all time frames. The color scale on the right indicates progression of time, where the first frame corresponds to 0:00 and the last frame is 85:00 (min:sec). Scale bar in bottom right of F = 5 microns and applies to E-F. G and H. To show a representation of dynamics of AO+ cell movement in association with microglia, a subset of AO+ cells were tracked in 3D using Imaris software. Quantifications show the speed (G) and total displacement (H) of individual AO+ cell bodies. Box plots are shown and circles are color coded to represent each embryo imaged. Each circle represents one AO+ cell that was tracked in each embryo that was imaged. Results are shown for n=6 different embryos.
    Figure Legend Snippet: A-D. Each circle is color coded for each embryo imaged, and overlaid onto box plots. A. Total number of AO+ cells observed during live imaging from ~54-62 hours post fertilization (hpf). B. The percent of AO+ cells that were observed to be engulfed by a microglial cell during the imaging session. C. For AO+ cells observed to be cleared by microglia, the percent showing association with a microglial cell at the onset of AO+ signal. D. Each circle represents individual cells from each embryo that was imaged. A selected number of individual AO+ cells, which were observed to appear and be cleared, during the imaging session were tracked in each embryo imaged. The duration of AO signal is shown for these individual AO+ cells. E-F represent max projections of selected, flattened z planes over a selected time-lapse. E. Shows the initial detection of an AO+ cell (green, indicated by red-orange arrow) associated with a microglial cell (microglia=magenta). E’. Shows the same AO+ cell (green, indicated by the magenta arrow) 80 minutes later, just before the AO signal is lost, after it had moved with the migrating microglial cell (magenta). F. The movement of the AO+ cell body was temporally color-coded to show its dynamic movement over time; the AO+ cell was associated with a microglial cell at all time frames. The color scale on the right indicates progression of time, where the first frame corresponds to 0:00 and the last frame is 85:00 (min:sec). Scale bar in bottom right of F = 5 microns and applies to E-F. G and H. To show a representation of dynamics of AO+ cell movement in association with microglia, a subset of AO+ cells were tracked in 3D using Imaris software. Quantifications show the speed (G) and total displacement (H) of individual AO+ cell bodies. Box plots are shown and circles are color coded to represent each embryo imaged. Each circle represents one AO+ cell that was tracked in each embryo that was imaged. Results are shown for n=6 different embryos.

    Techniques Used: Imaging, Software

    Related Articles

    Analysis:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Generated:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Morphology:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Software:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Z-Stacks:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Fluorescence Microscopy:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Fluorescence:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Microscopy:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Western Blot:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Incubation:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Drosophila:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Confocal:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Blocking Assay:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Imaging:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Cytometry:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Flow Cytometry:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Structured Illumination Microscopy:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Micro-CT:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Quantitative RT-PCR:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Gene Expression:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Expressing:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Confocal Laser Scanning Microscopy:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Next-Generation Sequencing:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Mouse Assay:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide

    Laser-Scanning Microscopy:

    Article Title: Red Fluorescent Labeling of Myelin by Membrane-Targeted tdTomato in Transgenic Mouse Lines.
    Article Snippet: adjusted for brightness and contrast with Fiji. For 3D reconstruction and morphological analysis of oligodendrocytes in line CNPMTO- C, z- stacks were acquired at 0.5 μm intervals and processed using Imaris (version 10.2) for 3D reconstruction (Surface mode) and myelin sheath measurements (Filament mode). Optic and sciatic nerves were whole- mounted (Fluoromount- G, Invitrogen) on SuperFrost slide



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