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live-cell 3d confocal microscopy  (Carl Zeiss)


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    Structured Review

    Carl Zeiss live-cell 3d confocal microscopy
    Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine <t>3D</t> trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative <t>fluorescence</t> <t>microscopy</t> images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.
    Live Cell 3d Confocal Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+tracking+algorithm/5+confocal+live+lsm+microscope/pmc04995943-330-7-13
    Average 90 stars, based on 1 article reviews
    live-cell 3d confocal microscopy - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Intratumoral oxygen gradients mediate sarcoma cell invasion"

    Article Title: Intratumoral oxygen gradients mediate sarcoma cell invasion

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1605317113

    Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine 3D trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative fluorescence microscopy images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.
    Figure Legend Snippet: Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine 3D trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative fluorescence microscopy images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.

    Techniques Used: Migration, Fluorescence, Microscopy, Western Blot, Cell Culture

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    Isolation:

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